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P0A6M4 (DTD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-tyrosyl-tRNA(Tyr) deacylase

EC=3.1.-.-
Gene names
Name:dtd
Synonyms:yihZ
Ordered Locus Names:b3887, JW3858
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length145 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine. Can also cleave D-aspartyl-tRNA(Asp) and D-tryptophanyl-tRNA(Trp). HAMAP-Rule MF_00518

Subunit structure

Homodimer.

Subcellular location

Cytoplasm Probable HAMAP-Rule MF_00518.

Sequence similarities

Belongs to the DTD family.

Ontologies

Keywords
   Cellular componentCytoplasm
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processD-amino acid catabolic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA metabolic process

Inferred from mutant phenotype Ref.4. Source: EcoCyc

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionD-tyrosyl-tRNA(Tyr) deacylase activity

Inferred from direct assay Ref.4. Source: EcoCyc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ccaP069611EBI-562575,EBI-545256

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 145145D-tyrosyl-tRNA(Tyr) deacylase HAMAP-Rule MF_00518
PRO_0000164537

Sites

Active site801Nucleophile By similarity

Secondary structure

......................... 145
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A6M4 [UniParc].

Last modified May 10, 2005. Version 1.
Checksum: 5CF0C0DB0819EC9B

FASTA14515,950
        10         20         30         40         50         60 
MIALIQRVTR ASVTVEGEVT GEIGAGLLVL LGVEKDDDEQ KANRLCERVL GYRIFSDAEG 

        70         80         90        100        110        120 
KMNLNVQQAG GSVLVVSQFT LAADTERGMR PSFSKGASPD RAEALYDYFV ERCRQQEMNT 

       130        140 
QTGRFAADMQ VSLVNDGPVT FWLQV 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Functional characterization of the D-Tyr-tRNATyr deacylase from Escherichia coli."
Soutourina J., Plateau P., Delort F., Peirotes A., Blanquet S.
J. Biol. Chem. 274:19109-19114(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION, PROTEIN SEQUENCE OF 1-10.
Strain: K12 / K37.
[5]"Metabolism of D-aminoacyl-tRNAs in Escherichia coli and Saccharomyces cerevisiae cells."
Soutourina J., Plateau P., Blanquet S.
J. Biol. Chem. 275:32535-32542(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: K12 / K37.
[6]"Structure of crystalline D-Tyr-tRNA(Tyr) deacylase. A representative of a new class of tRNA-dependent hydrolases."
Ferri-Fioni M.-L., Schmitt E., Soutourina J., Plateau P., Mechulam Y., Blanquet S.
J. Biol. Chem. 276:47285-47290(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS).
Strain: K12 / K37.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L19201 Genomic DNA. Translation: AAB03020.1.
U00096 Genomic DNA. Translation: AAD13449.1.
AP009048 Genomic DNA. Translation: BAE77422.1.
PIRS40831.
RefSeqNP_418323.1. NC_000913.3.
YP_491563.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JKEX-ray1.55A/B/C/D1-145[»]
ProteinModelPortalP0A6M4.
SMRP0A6M4. Positions 1-145.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-47962N.
IntActP0A6M4. 9 interactions.
STRING511145.b3887.

Proteomic databases

PaxDbP0A6M4.
PRIDEP0A6M4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAD13449; AAD13449; b3887.
BAE77422; BAE77422; BAE77422.
GeneID12933646.
948378.
KEGGecj:Y75_p3299.
eco:b3887.
PATRIC32123277. VBIEscCol129921_3999.

Organism-specific databases

EchoBASEEB1798.
EcoGeneEG11852. dtd.

Phylogenomic databases

eggNOGCOG1490.
HOGENOMHOG000113982.
KOK07560.
OMADGPVTIW.
OrthoDBEOG6C2WM2.
PhylomeDBP0A6M4.
ProtClustDBPRK05273.

Enzyme and pathway databases

BioCycEcoCyc:EG11852-MONOMER.
ECOL316407:JW3858-MONOMER.

Gene expression databases

GenevestigatorP0A6M4.

Family and domain databases

Gene3D3.50.80.10. 1 hit.
HAMAPMF_00518. Tyr_Deacylase_Dtd.
InterProIPR023509. DTD-like_dom.
IPR003732. DTyrtRNA_deacyls.
[Graphical view]
PANTHERPTHR10472. PTHR10472. 1 hit.
PfamPF02580. Tyr_Deacylase. 1 hit.
[Graphical view]
SUPFAMSSF69500. SSF69500. 1 hit.
TIGRFAMsTIGR00256. TIGR00256. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP0A6M4.
PROP0A6M4.

Entry information

Entry nameDTD_ECOLI
AccessionPrimary (citable) accession number: P0A6M4
Secondary accession number(s): P32147, Q2M8I4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: April 16, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene