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Protein

Disulfide bond formation protein B

Gene

dsbB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.3 Publications

GO - Molecular functioni

  • protein disulfide oxidoreductase activity Source: EcoCyc

GO - Biological processi

  • electron transport chain Source: EcoCyc
  • response to heat Source: EcoCyc

Keywordsi

Molecular functionChaperone, Oxidoreductase
Biological processElectron transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:DSBBPROT-MONOMER
MetaCyc:DSBBPROT-MONOMER

Protein family/group databases

TCDBi5.A.2.1.1 the disulfide bond oxidoreductase b (dsbb) family

Names & Taxonomyi

Protein namesi
Recommended name:
Disulfide bond formation protein B
Alternative name(s):
Disulfide oxidoreductase
Gene namesi
Name:dsbB
Synonyms:roxB, ycgA
Ordered Locus Names:b1185, JW5182
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11393 dsbB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 14CytoplasmicCuratedAdd BLAST14
Transmembranei15 – 31HelicalCuratedAdd BLAST17
Topological domaini32 – 49PeriplasmicCuratedAdd BLAST18
Transmembranei50 – 65HelicalCuratedAdd BLAST16
Topological domaini66 – 71CytoplasmicCurated6
Transmembranei72 – 89HelicalCuratedAdd BLAST18
Topological domaini90 – 144PeriplasmicCuratedAdd BLAST55
Transmembranei145 – 163HelicalCuratedAdd BLAST19
Topological domaini164 – 176CytoplasmicCuratedAdd BLAST13

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Induction of the PhoP/PhoQ two-component regulatory system.1 Publication

Chemistry databases

DrugBankiDB08689 UBIQUINONE-1
DB08690 UBIQUINONE-2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000593421 – 176Disulfide bond formation protein BAdd BLAST176

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi41 ↔ 44Redox-active1 Publication
Disulfide bondi104 ↔ 130Redox-active1 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP0A6M2
PRIDEiP0A6M2

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
dsbAP0AEG45EBI-1170740,EBI-549711

Protein-protein interaction databases

BioGridi4261815, 428 interactors
IntActiP0A6M2, 1 interactor
MINTiP0A6M2
STRINGi316385.ECDH10B_1238

Chemistry databases

BindingDBiP0A6M2

Structurei

Secondary structure

1176
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 9Combined sources8
Helixi13 – 21Combined sources9
Helixi23 – 34Combined sources12
Helixi44 – 62Combined sources19
Beta strandi66 – 69Combined sources4
Helixi70 – 96Combined sources27
Helixi115 – 118Combined sources4
Turni122 – 124Combined sources3
Beta strandi138 – 140Combined sources3
Helixi142 – 157Combined sources16
Turni158 – 161Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2HI7X-ray3.70B1-176[»]
2K73NMR-A1-176[»]
2K74NMR-A1-176[»]
2LEGNMR-B1-176[»]
2LTQNMR-A/D1-176[»]
2ZUPX-ray3.70B1-176[»]
2ZUQX-ray3.30A/D1-176[»]
3E9JX-ray3.70C/F1-176[»]
ProteinModelPortaliP0A6M2
SMRiP0A6M2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6M2

Family & Domainsi

Sequence similaritiesi

Belongs to the DsbB family.Curated

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410635M Bacteria
COG1495 LUCA
HOGENOMiHOG000218135
InParanoidiP0A6M2
KOiK03611
OMAiCILQRYA

Family and domain databases

Gene3Di1.20.1550.10, 1 hit
HAMAPiMF_00286 DsbB, 1 hit
InterProiView protein in InterPro
IPR003752 DiS_bond_form_DsbB/BdbC
IPR022920 Disulphide_bond_form_DsbB
IPR023380 DsbB-like_sf
PfamiView protein in Pfam
PF02600 DsbB, 1 hit
SUPFAMiSSF158442 SSF158442, 1 hit

Sequencei

Sequence statusi: Complete.

P0A6M2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRFLNQCSQ GRGAWLLMAF TALALELTAL WFQHVMLLKP CVLCIYERCA
60 70 80 90 100
LFGVLGAALI GAIAPKTPLR YVAMVIWLYS AFRGVQLTYE HTMLQLYPSP
110 120 130 140 150
FATCDFMVRF PEWLPLDKWV PQVFVASGDC AERQWDFLGL EMPQWLLGIF
160 170
IAYLIVAVLV VISQPFKAKK RDLFGR
Length:176
Mass (Da):20,142
Last modified:May 10, 2005 - v1
Checksum:i9CBD673D51E9F09B
GO

Sequence cautioni

The sequence AAA23711 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence AAA24220 differs from that shown. Reason: Frameshift at position 130.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03721 Genomic DNA Translation: AAA23711.1 Different initiation.
U00096 Genomic DNA Translation: AAC74269.1
AP009048 Genomic DNA Translation: BAA36032.2
M83655 Genomic DNA Translation: AAA24220.1 Frameshift.
PIRiF64864
RefSeqiNP_415703.3, NC_000913.3
WP_000943459.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74269; AAC74269; b1185
BAA36032; BAA36032; BAA36032
GeneIDi946344
KEGGiecj:JW5182
eco:b1185
PATRICifig|1411691.4.peg.1102

Similar proteinsi

Entry informationi

Entry nameiDSBB_ECOLI
AccessioniPrimary (citable) accession number: P0A6M2
Secondary accession number(s): P30018, Q47408
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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