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Protein

Co-chaperone protein HscB

Gene

hscB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Co-chaperone involved in the maturation of iron-sulfur cluster-containing proteins. Seems to help targeting proteins to be folded toward HscA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Enzyme and pathway databases

BioCyciEcoCyc:EG12131-MONOMER.
ECOL316407:JW2511-MONOMER.
MetaCyc:EG12131-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Co-chaperone protein HscB
Alternative name(s):
Hsc20
Gene namesi
Name:hscB
Synonyms:yfhE
Ordered Locus Names:b2527, JW2511
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12131. hscB.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • iron-sulfur cluster transfer complex Source: EcoCyc
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 171171Co-chaperone protein HscBPRO_0000070966Add
BLAST

Proteomic databases

EPDiP0A6L9.
PaxDbiP0A6L9.
PRIDEiP0A6L9.

Interactioni

Subunit structurei

Interacts with HscA and stimulates its ATPase activity. Interacts with IscU.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4263037. 42 interactions.
DIPiDIP-47847N.
IntActiP0A6L9. 20 interactions.
STRINGi511145.b2527.

Structurei

Secondary structure

1
171
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 65Combined sources
Beta strandi11 – 133Combined sources
Helixi17 – 3014Combined sources
Helixi33 – 364Combined sources
Helixi41 – 6222Combined sources
Helixi64 – 7310Combined sources
Turni74 – 763Combined sources
Beta strandi82 – 843Combined sources
Helixi89 – 10820Combined sources
Helixi111 – 13727Combined sources
Helixi141 – 16525Combined sources
Turni166 – 1694Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FPOX-ray1.80A/B/C1-171[»]
ProteinModelPortaliP0A6L9.
SMRiP0A6L9. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6L9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7473JAdd
BLAST

Sequence similaritiesi

Belongs to the HscB family.Curated
Contains 1 J domain.Curated

Phylogenomic databases

eggNOGiENOG4108SWN. Bacteria.
COG1076. LUCA.
HOGENOMiHOG000262077.
InParanoidiP0A6L9.
KOiK04082.
OMAiFNPFQIF.
OrthoDBiEOG66F07J.
PhylomeDBiP0A6L9.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
1.20.1280.20. 1 hit.
HAMAPiMF_00682. HscB.
InterProiIPR001623. DnaJ_domain.
IPR004640. HscB.
IPR009073. HscB_oligo_C.
[Graphical view]
PANTHERiPTHR14021. PTHR14021. 1 hit.
PfamiPF07743. HSCB_C. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF47144. SSF47144. 1 hit.
TIGRFAMsiTIGR00714. hscB. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6L9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYFTLFGLP ARYQLDTQAL SLRFQDLQRQ YHPDKFASGS QAEQLAAVQQ
60 70 80 90 100
SATINQAWQT LRHPLMRAEY LLSLHGFDLA SEQHTVRDTA FLMEQLELRE
110 120 130 140 150
ELDEIEQAKD EARLESFIKR VKKMFDTRHQ LMVEQLDNET WDAAADTVRK
160 170
LRFLDKLRSS AEQLEEKLLD F
Length:171
Mass (Da):20,138
Last modified:May 10, 2005 - v1
Checksum:i095193EE98AA60C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA. Translation: AAA18299.1.
U00096 Genomic DNA. Translation: AAC75580.1.
AP009048 Genomic DNA. Translation: BAA16421.1.
U05338 Genomic DNA. No translation available.
PIRiA36958.
RefSeqiNP_417022.1. NC_000913.3.
WP_000384413.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75580; AAC75580; b2527.
BAA16421; BAA16421; BAA16421.
GeneIDi946995.
KEGGiecj:JW2511.
eco:b2527.
PATRICi32120449. VBIEscCol129921_2627.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U01827 Unassigned DNA. Translation: AAA18299.1.
U00096 Genomic DNA. Translation: AAC75580.1.
AP009048 Genomic DNA. Translation: BAA16421.1.
U05338 Genomic DNA. No translation available.
PIRiA36958.
RefSeqiNP_417022.1. NC_000913.3.
WP_000384413.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1FPOX-ray1.80A/B/C1-171[»]
ProteinModelPortaliP0A6L9.
SMRiP0A6L9. Positions 1-171.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263037. 42 interactions.
DIPiDIP-47847N.
IntActiP0A6L9. 20 interactions.
STRINGi511145.b2527.

Proteomic databases

EPDiP0A6L9.
PaxDbiP0A6L9.
PRIDEiP0A6L9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75580; AAC75580; b2527.
BAA16421; BAA16421; BAA16421.
GeneIDi946995.
KEGGiecj:JW2511.
eco:b2527.
PATRICi32120449. VBIEscCol129921_2627.

Organism-specific databases

EchoBASEiEB2052.
EcoGeneiEG12131. hscB.

Phylogenomic databases

eggNOGiENOG4108SWN. Bacteria.
COG1076. LUCA.
HOGENOMiHOG000262077.
InParanoidiP0A6L9.
KOiK04082.
OMAiFNPFQIF.
OrthoDBiEOG66F07J.
PhylomeDBiP0A6L9.

Enzyme and pathway databases

BioCyciEcoCyc:EG12131-MONOMER.
ECOL316407:JW2511-MONOMER.
MetaCyc:EG12131-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP0A6L9.
PROiP0A6L9.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
1.20.1280.20. 1 hit.
HAMAPiMF_00682. HscB.
InterProiIPR001623. DnaJ_domain.
IPR004640. HscB.
IPR009073. HscB_oligo_C.
[Graphical view]
PANTHERiPTHR14021. PTHR14021. 1 hit.
PfamiPF07743. HSCB_C. 1 hit.
[Graphical view]
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF47144. SSF47144. 1 hit.
TIGRFAMsiTIGR00714. hscB. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutations in a gene encoding a new Hsp70 suppress rapid DNA inversion and bgl activation, but not proU derepression, in hns-1 mutant Escherichia coli."
    Kawula T.H., Lelivelt M.J.
    J. Bacteriol. 176:610-619(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A gene encoding a DnaK/hsp70 homolog in Escherichia coli."
    Seaton B.L., Vickery L.E.
    Proc. Natl. Acad. Sci. U.S.A. 91:2066-2070(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 119-171.
    Strain: B.
  6. "Hsc66 and Hsc20, a new heat shock cognate molecular chaperone system from Escherichia coli."
    Vickery L.E., Silberg J.J., Ta D.T.
    Protein Sci. 6:1047-1056(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. "Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichia coli."
    Hoff K.G., Silberg J.J., Vickery L.E.
    Proc. Natl. Acad. Sci. U.S.A. 97:7790-7795(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  8. "Crystallization and preliminary X-ray crystallographic properties of Hsc20, a J-motif co-chaperone protein from Escherichia coli."
    Cupp-Vickery J.R., Vickery L.E.
    Protein Sci. 6:2028-2030(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: CRYSTALLIZATION.
  9. "Crystal structure of Hsc20, a J-type co-chaperone from Escherichia coli."
    Cupp-Vickery J.R., Vickery L.E.
    J. Mol. Biol. 304:835-845(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).

Entry informationi

Entry nameiHSCB_ECOLI
AccessioniPrimary (citable) accession number: P0A6L9
Secondary accession number(s): P36540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: March 16, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.