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P0A6J8

- DDLA_ECOLI

UniProt

P0A6J8 - DDLA_ECOLI

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Protein

D-alanine--D-alanine ligase A

Gene

ddlA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Cell wall formation.

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.

Cofactori

Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi302 – 3021Magnesium or manganese 1By similarity
Metal bindingi315 – 3151Magnesium or manganese 1By similarity
Metal bindingi315 – 3151Magnesium or manganese 2By similarity
Metal bindingi317 – 3171Magnesium or manganese 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi175 – 23056ATPBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. D-alanine-D-alanine ligase activity Source: EcoCyc
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. manganese ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. cell wall organization Source: UniProtKB-KW
  2. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
  3. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:DALADALALIGA-MONOMER.
ECOL316407:JW0372-MONOMER.
MetaCyc:DALADALALIGA-MONOMER.
UniPathwayiUPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligase A (EC:6.3.2.4)
Alternative name(s):
D-Ala-D-Ala ligase A
D-alanylalanine synthetase A
Gene namesi
Name:ddlA
Ordered Locus Names:b0381, JW0372
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10213. ddlA.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 364364D-alanine--D-alanine ligase APRO_0000177815Add
BLAST

Proteomic databases

PaxDbiP0A6J8.
PRIDEiP0A6J8.

Expressioni

Gene expression databases

GenevestigatoriP0A6J8.

Interactioni

Protein-protein interaction databases

DIPiDIP-47939N.
IntActiP0A6J8. 14 interactions.
STRINGi511145.b0381.

Structurei

3D structure databases

ProteinModelPortaliP0A6J8.
SMRiP0A6J8. Positions 3-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini145 – 348204ATP-graspAdd
BLAST

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.Curated
Contains 1 ATP-grasp domain.Curated

Phylogenomic databases

eggNOGiCOG1181.
HOGENOMiHOG000011593.
InParanoidiP0A6J8.
KOiK01921.
OMAiQIDVIFP.
OrthoDBiEOG64BQ73.
PhylomeDBiP0A6J8.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPiMF_00047. Dala_Dala_lig.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERiPTHR23132. PTHR23132. 1 hit.
PfamiPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMiSSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6J8 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEKLRVGIVF GGKSAEHEVS LQSAKNIVDA IDKSRFDVVL LGIDKQGQWH
60 70 80 90 100
VSDASNYLLN ADDPAHIALR PSATSLAQVP GKHEHQLIDA QNGQPLPTVD
110 120 130 140 150
VIFPIVHGTL GEDGSLQGML RVANLPFVGS DVLASAACMD KDVTKRLLRD
160 170 180 190 200
AGLNIAPFIT LTRANRHNIS FAEVESKLGL PLFVKPANQG SSVGVSKVTS
210 220 230 240 250
EEQYAIAVDL AFEFDHKVIV EQGIKGREIE CAVLGNDNPQ ASTCGEIVLT
260 270 280 290 300
SDFYAYDTKY IDEDGAKVVV PAAIAPEIND KIRAIAVQAY QTLGCAGMAR
310 320 330 340 350
VDVFLTPENE VVINEINTLP GFTNISMYPK LWQASGLGYT DLITRLIELA
360
LERHAADNAL KTTM
Length:364
Mass (Da):39,316
Last modified:March 29, 2005 - v1
Checksum:iC7FDBEC353AC1320
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58467 Genomic DNA. Translation: AAA23671.1.
U73857 Genomic DNA. Translation: AAB18104.1.
U00096 Genomic DNA. Translation: AAC73484.1.
AP009048 Genomic DNA. Translation: BAE76162.1.
PIRiA39182. CEECDA.
RefSeqiNP_414915.1. NC_000913.3.
YP_488674.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73484; AAC73484; b0381.
BAE76162; BAE76162; BAE76162.
GeneIDi12932537.
945313.
KEGGiecj:Y75_p0370.
eco:b0381.
PATRICi32115903. VBIEscCol129921_0393.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M58467 Genomic DNA. Translation: AAA23671.1 .
U73857 Genomic DNA. Translation: AAB18104.1 .
U00096 Genomic DNA. Translation: AAC73484.1 .
AP009048 Genomic DNA. Translation: BAE76162.1 .
PIRi A39182. CEECDA.
RefSeqi NP_414915.1. NC_000913.3.
YP_488674.1. NC_007779.1.

3D structure databases

ProteinModelPortali P0A6J8.
SMRi P0A6J8. Positions 3-363.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-47939N.
IntActi P0A6J8. 14 interactions.
STRINGi 511145.b0381.

Chemistry

DrugBanki DB00260. Cycloserine.

Proteomic databases

PaxDbi P0A6J8.
PRIDEi P0A6J8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73484 ; AAC73484 ; b0381 .
BAE76162 ; BAE76162 ; BAE76162 .
GeneIDi 12932537.
945313.
KEGGi ecj:Y75_p0370.
eco:b0381.
PATRICi 32115903. VBIEscCol129921_0393.

Organism-specific databases

EchoBASEi EB0209.
EcoGenei EG10213. ddlA.

Phylogenomic databases

eggNOGi COG1181.
HOGENOMi HOG000011593.
InParanoidi P0A6J8.
KOi K01921.
OMAi QIDVIFP.
OrthoDBi EOG64BQ73.
PhylomeDBi P0A6J8.

Enzyme and pathway databases

UniPathwayi UPA00219 .
BioCyci EcoCyc:DALADALALIGA-MONOMER.
ECOL316407:JW0372-MONOMER.
MetaCyc:DALADALALIGA-MONOMER.

Miscellaneous databases

PROi P0A6J8.

Gene expression databases

Genevestigatori P0A6J8.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPi MF_00047. Dala_Dala_lig.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view ]
PANTHERi PTHR23132. PTHR23132. 1 hit.
Pfami PF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view ]
SUPFAMi SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes."
    Zawadzke L.E., Bugg T.D., Walsh C.T.
    Biochemistry 30:1673-1682(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequence of minutes 4-25 of Escherichia coli."
    Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Entry informationi

Entry nameiDDLA_ECOLI
AccessioniPrimary (citable) accession number: P0A6J8
Secondary accession number(s): P23844, Q2MC44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: October 29, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3