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P0A6J8

- DDLA_ECOLI

UniProt

P0A6J8 - DDLA_ECOLI

Protein

D-alanine--D-alanine ligase A

Gene

ddlA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 94 (01 Oct 2014)
      Sequence version 1 (29 Mar 2005)
      Previous versions | rss
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    Functioni

    Cell wall formation.

    Catalytic activityi

    ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.

    Cofactori

    Binds 2 magnesium or manganese ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi302 – 3021Magnesium or manganese 1By similarity
    Metal bindingi315 – 3151Magnesium or manganese 1By similarity
    Metal bindingi315 – 3151Magnesium or manganese 2By similarity
    Metal bindingi317 – 3171Magnesium or manganese 2By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi175 – 23056ATPBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. D-alanine-D-alanine ligase activity Source: EcoCyc
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. manganese ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. peptidoglycan biosynthetic process Source: UniProtKB-HAMAP
    2. regulation of cell shape Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Keywords - Ligandi

    ATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:DALADALALIGA-MONOMER.
    ECOL316407:JW0372-MONOMER.
    MetaCyc:DALADALALIGA-MONOMER.
    UniPathwayiUPA00219.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-alanine--D-alanine ligase A (EC:6.3.2.4)
    Alternative name(s):
    D-Ala-D-Ala ligase A
    D-alanylalanine synthetase A
    Gene namesi
    Name:ddlA
    Ordered Locus Names:b0381, JW0372
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10213. ddlA.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 364364D-alanine--D-alanine ligase APRO_0000177815Add
    BLAST

    Proteomic databases

    PaxDbiP0A6J8.
    PRIDEiP0A6J8.

    Expressioni

    Gene expression databases

    GenevestigatoriP0A6J8.

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-47939N.
    IntActiP0A6J8. 14 interactions.
    STRINGi511145.b0381.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6J8.
    SMRiP0A6J8. Positions 3-363.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini145 – 348204ATP-graspAdd
    BLAST

    Sequence similaritiesi

    Belongs to the D-alanine--D-alanine ligase family.Curated
    Contains 1 ATP-grasp domain.Curated

    Phylogenomic databases

    eggNOGiCOG1181.
    HOGENOMiHOG000011593.
    KOiK01921.
    OMAiQIDVIFP.
    OrthoDBiEOG64BQ73.
    PhylomeDBiP0A6J8.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPiMF_00047. Dala_Dala_lig.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view]
    PANTHERiPTHR23132. PTHR23132. 1 hit.
    PfamiPF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A6J8-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKLRVGIVF GGKSAEHEVS LQSAKNIVDA IDKSRFDVVL LGIDKQGQWH    50
    VSDASNYLLN ADDPAHIALR PSATSLAQVP GKHEHQLIDA QNGQPLPTVD 100
    VIFPIVHGTL GEDGSLQGML RVANLPFVGS DVLASAACMD KDVTKRLLRD 150
    AGLNIAPFIT LTRANRHNIS FAEVESKLGL PLFVKPANQG SSVGVSKVTS 200
    EEQYAIAVDL AFEFDHKVIV EQGIKGREIE CAVLGNDNPQ ASTCGEIVLT 250
    SDFYAYDTKY IDEDGAKVVV PAAIAPEIND KIRAIAVQAY QTLGCAGMAR 300
    VDVFLTPENE VVINEINTLP GFTNISMYPK LWQASGLGYT DLITRLIELA 350
    LERHAADNAL KTTM 364
    Length:364
    Mass (Da):39,316
    Last modified:March 29, 2005 - v1
    Checksum:iC7FDBEC353AC1320
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58467 Genomic DNA. Translation: AAA23671.1.
    U73857 Genomic DNA. Translation: AAB18104.1.
    U00096 Genomic DNA. Translation: AAC73484.1.
    AP009048 Genomic DNA. Translation: BAE76162.1.
    PIRiA39182. CEECDA.
    RefSeqiNP_414915.1. NC_000913.3.
    YP_488674.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC73484; AAC73484; b0381.
    BAE76162; BAE76162; BAE76162.
    GeneIDi12932537.
    945313.
    KEGGiecj:Y75_p0370.
    eco:b0381.
    PATRICi32115903. VBIEscCol129921_0393.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M58467 Genomic DNA. Translation: AAA23671.1 .
    U73857 Genomic DNA. Translation: AAB18104.1 .
    U00096 Genomic DNA. Translation: AAC73484.1 .
    AP009048 Genomic DNA. Translation: BAE76162.1 .
    PIRi A39182. CEECDA.
    RefSeqi NP_414915.1. NC_000913.3.
    YP_488674.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P0A6J8.
    SMRi P0A6J8. Positions 3-363.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-47939N.
    IntActi P0A6J8. 14 interactions.
    STRINGi 511145.b0381.

    Chemistry

    DrugBanki DB00260. Cycloserine.

    Proteomic databases

    PaxDbi P0A6J8.
    PRIDEi P0A6J8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC73484 ; AAC73484 ; b0381 .
    BAE76162 ; BAE76162 ; BAE76162 .
    GeneIDi 12932537.
    945313.
    KEGGi ecj:Y75_p0370.
    eco:b0381.
    PATRICi 32115903. VBIEscCol129921_0393.

    Organism-specific databases

    EchoBASEi EB0209.
    EcoGenei EG10213. ddlA.

    Phylogenomic databases

    eggNOGi COG1181.
    HOGENOMi HOG000011593.
    KOi K01921.
    OMAi QIDVIFP.
    OrthoDBi EOG64BQ73.
    PhylomeDBi P0A6J8.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .
    BioCyci EcoCyc:DALADALALIGA-MONOMER.
    ECOL316407:JW0372-MONOMER.
    MetaCyc:DALADALALIGA-MONOMER.

    Miscellaneous databases

    PROi P0A6J8.

    Gene expression databases

    Genevestigatori P0A6J8.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    HAMAPi MF_00047. Dala_Dala_lig.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR000291. D-Ala_lig_Van_CS.
    IPR005905. D_ala_D_ala.
    IPR011095. Dala_Dala_lig_C.
    IPR011127. Dala_Dala_lig_N.
    IPR016185. PreATP-grasp_dom.
    [Graphical view ]
    PANTHERi PTHR23132. PTHR23132. 1 hit.
    Pfami PF07478. Dala_Dala_lig_C. 1 hit.
    PF01820. Dala_Dala_lig_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR01205. D_ala_D_alaTIGR. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00843. DALA_DALA_LIGASE_1. 1 hit.
    PS00844. DALA_DALA_LIGASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Existence of two D-alanine:D-alanine ligases in Escherichia coli: cloning and sequencing of the ddlA gene and purification and characterization of the DdlA and DdlB enzymes."
      Zawadzke L.E., Bugg T.D., Walsh C.T.
      Biochemistry 30:1673-1682(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequence of minutes 4-25 of Escherichia coli."
      Chung E., Allen E., Araujo R., Aparicio A.M., Davis K., Duncan M., Federspiel N., Hyman R., Kalman S., Komp C., Kurdi O., Lew H., Lin D., Namath A., Oefner P., Roberts D., Schramm S., Davis R.W.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

    Entry informationi

    Entry nameiDDLA_ECOLI
    AccessioniPrimary (citable) accession number: P0A6J8
    Secondary accession number(s): P23844, Q2MC44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: October 1, 2014
    This is version 94 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3