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Protein

D-amino acid dehydrogenase

Gene

dadA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidative deamination of D-amino acids. Has broad substrate specificity; is mostly active on D-alanine, and to a lesser extent, on several other D-amino acids such as D-methionine, D-serine and D-proline, but not on L-alanine. Participates in the utilization of L-alanine and D-alanine as the sole source of carbon, nitrogen and energy for growth. Is also able to oxidize D-amino acid analogs such as 3,4-dehydro-D-proline, D-2-aminobutyrate, D-norvaline, D-norleucine, cis-4-hydroxy-D-proline, and DL-ethionine.2 Publications

Caution

Was originally thought to be a heterodimer based on the purification of the enzyme first reported from E.coli B, but results of enzyme assays in PubMed:21378189 have indicated that DadA is solely responsible for the observed dehydrogenase activity.Curated

Catalytic activityi

A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor.2 Publications

Cofactori

FADBy similarity

Enzyme regulationi

Is activated by the HinT protein. Is inhibited by D-cycloserine.2 Publications

Kineticsi

  1. KM=30 mM for D-alanine2 Publications
  2. KM=6.4 mM for 3,4-dehydro-D-proline2 Publications

    pH dependencei

    Optimum pH is about 8.9 with D-alanine as substrate and about 9 with 3,4-dehydro-D-proline as substrate.2 Publications

    Pathwayi: D-alanine degradation

    This protein is involved in step 1 of the subpathway that synthesizes NH(3) and pyruvate from D-alanine.
    Proteins known to be involved in this subpathway in this organism are:
    1. D-amino acid dehydrogenase (dadA)
    This subpathway is part of the pathway D-alanine degradation, which is itself part of Amino-acid degradation.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes NH(3) and pyruvate from D-alanine, the pathway D-alanine degradation and in Amino-acid degradation.

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi3 – 17FADSequence analysisAdd BLAST15

    GO - Molecular functioni

    • D-amino-acid dehydrogenase activity Source: EcoCyc

    GO - Biological processi

    • D-alanine catabolic process Source: EcoCyc
    • L-alanine oxidation to pyruvate via D-alanine Source: EcoCyc

    Keywordsi

    Molecular functionOxidoreductase
    LigandFAD, Flavoprotein

    Enzyme and pathway databases

    BioCyciEcoCyc:DALADEHYDROGA-MONOMER
    MetaCyc:DALADEHYDROGA-MONOMER
    SABIO-RKiP0A6J5
    UniPathwayiUPA00043; UER00498

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-amino acid dehydrogenase (EC:1.4.99.-2 Publications)
    Alternative name(s):
    D-alanine dehydrogenase
    Gene namesi
    Name:dadA
    Synonyms:dadR
    Ordered Locus Names:b1189, JW1178
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11407 dadA

    Subcellular locationi

    • Cell inner membrane 2 Publications; Peripheral membrane protein 2 Publications

    GO - Cellular componenti

    • cytoplasm Source: GO_Central
    • plasma membrane Source: EcoCyc

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Loss of the ability to utilize both D- and L-stereoisomers of alanine as sole sources of carbon, nitrogen and energy for growth (PubMed:13292). A double dtd-dadA deletion mutant has a pronounced growth defect in the presence of D-tyrosine (PubMed:10383414).2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001661311 – 432D-amino acid dehydrogenaseAdd BLAST432

    Proteomic databases

    EPDiP0A6J5
    PaxDbiP0A6J5
    PRIDEiP0A6J5

    Expressioni

    Inductioni

    By D-alanine. Is regulated by catabolite repression.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi4260104, 29 interactors
    DIPiDIP-6852N
    IntActiP0A6J5, 21 interactors
    STRINGi316385.ECDH10B_1242

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6J5
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the DadA oxidoreductase family.Curated

    Phylogenomic databases

    eggNOGiENOG4105MWK Bacteria
    COG0665 LUCA
    HOGENOMiHOG000217450
    InParanoidiP0A6J5
    KOiK00285
    OMAiYSITFKM
    PhylomeDBiP0A6J5

    Family and domain databases

    Gene3Di3.50.50.60, 4 hits
    HAMAPiMF_01202 DadA, 1 hit
    InterProiView protein in InterPro
    IPR023080 DadA
    IPR006076 FAD-dep_OxRdtase
    IPR036188 FAD/NAD-bd_sf
    PfamiView protein in Pfam
    PF01266 DAO, 1 hit
    SUPFAMiSSF51905 SSF51905, 3 hits

    Sequencei

    Sequence statusi: Complete.

    P0A6J5-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MRVVILGSGV VGVASAWYLN QAGHEVTVID REPGAALETS AANAGQISPG
    60 70 80 90 100
    YAAPWAAPGV PLKAIKWMFQ RHAPLAVRLD GTQFQLKWMW QMLRNCDTSH
    110 120 130 140 150
    YMENKGRMVR LAEYSRDCLK ALRAETNIQY EGRQGGTLQL FRTEQQYENA
    160 170 180 190 200
    TRDIAVLEDA GVPYQLLESS RLAEVEPALA EVAHKLTGGL QLPNDETGDC
    210 220 230 240 250
    QLFTQNLARM AEQAGVKFRF NTPVDQLLCD GEQIYGVKCG DEVIKADAYV
    260 270 280 290 300
    MAFGSYSTAM LKGIVDIPVY PLKGYSLTIP IAQEDGAPVS TILDETYKIA
    310 320 330 340 350
    ITRFDNRIRV GGMAEIVGFN TELLQPRRET LEMVVRDLYP RGGHVEQATF
    360 370 380 390 400
    WTGLRPMTPD GTPVVGRTRF KNLWLNTGHG TLGWTMACGS GQLLSDLLSG
    410 420 430
    RTPAIPYEDL SVARYSRGFT PSRPGHLHGA HS
    Length:432
    Mass (Da):47,607
    Last modified:March 29, 2005 - v1
    Checksum:iEE747358845B6280
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L02948 Unassigned DNA Translation: AAC36880.1
    U00096 Genomic DNA Translation: AAC74273.1
    AP009048 Genomic DNA Translation: BAA36044.1
    PIRiB53383
    RefSeqiNP_415707.1, NC_000913.3
    WP_001266908.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC74273; AAC74273; b1189
    BAA36044; BAA36044; BAA36044
    GeneIDi945752
    KEGGiecj:JW1178
    eco:b1189
    PATRICifig|1411691.4.peg.1098

    Similar proteinsi

    Entry informationi

    Entry nameiDADA_ECOLI
    AccessioniPrimary (citable) accession number: P0A6J5
    Secondary accession number(s): P29011
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: March 28, 2018
    This is version 109 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health