Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P0A6J5 (DADA_ECOLI)

Last modified November 3, 2009. Version 42. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1
Gene names
Name: dadA
Synonyms: dadR
Ordered Locus Names: b1189, JW1178
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Hide | Top

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

Oxidative deamination of D-amino acids. HAMAP MF_01202

Catalytic activity

A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202

Cofactor

FAD. HAMAP MF_01202

Nonheme iron. HAMAP MF_01202

Pathway

Amino-acid degradation; D-alanine degradation; NH(3) and pyruvate from D-alanine: step 1/1. HAMAP MF_01202

Subunit structure

Heterodimer of a small and a large subunit. HAMAP MF_01202

Subcellular location

Cell inner membrane; Peripheral membrane protein. HAMAP MF_01202

Induction

By alanine. HAMAP MF_01202

Sequence similarities

Belongs to the dadA oxidoreductase family.

Hide | Top

Ontologies

Keywords
   Cellular componentCell inner membrane
Cell membrane
Membrane
   LigandFAD
Flavoprotein
Iron
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processalanine catabolic process

Inferred from electronic annotation. Source: HAMAP

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextrinsic to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionD-amino-acid dehydrogenase activity

Inferred from electronic annotation. Source: HAMAP

iron ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 432432D-amino acid dehydrogenase small subunit HAMAP MF_01202
PRO_0000166131

Regions

Nucleotide binding3 – 1715FAD Potential

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P0A6J5-1 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: EE747358845B6280

FASTA43247,607
        10         20         30         40         50         60 
MRVVILGSGV VGVASAWYLN QAGHEVTVID REPGAALETS AANAGQISPG YAAPWAAPGV 

        70         80         90        100        110        120 
PLKAIKWMFQ RHAPLAVRLD GTQFQLKWMW QMLRNCDTSH YMENKGRMVR LAEYSRDCLK 

       130        140        150        160        170        180 
ALRAETNIQY EGRQGGTLQL FRTEQQYENA TRDIAVLEDA GVPYQLLESS RLAEVEPALA 

       190        200        210        220        230        240 
EVAHKLTGGL QLPNDETGDC QLFTQNLARM AEQAGVKFRF NTPVDQLLCD GEQIYGVKCG 

       250        260        270        280        290        300 
DEVIKADAYV MAFGSYSTAM LKGIVDIPVY PLKGYSLTIP IAQEDGAPVS TILDETYKIA 

       310        320        330        340        350        360 
ITRFDNRIRV GGMAEIVGFN TELLQPRRET LEMVVRDLYP RGGHVEQATF WTGLRPMTPD 

       370        380        390        400        410        420 
GTPVVGRTRF KNLWLNTGHG TLGWTMACGS GQLLSDLLSG RTPAIPYEDL SVARYSRGFT 

       430 
PSRPGHLHGA HS

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Organization and expression of the Escherichia coli K-12 dad operon encoding the smaller subunit of D-amino acid dehydrogenase and the catabolic alanine racemase."
Lobocka M., Hennig J., Wild J., Klopotowski T.
J. Bacteriol. 176:1500-1510(1994) [PubMed: 7906689] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Hide | Top

Cross-references

Sequence databases

L02948 Unassigned DNA. Translation: AAC36880.1.
U00096 Genomic DNA. Translation: AAC74273.1.
AP009048 Genomic DNA. Translation: BAA36044.1.
PIRB53383.
RefSeqAP_001814.1.
NP_415707.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGP0A6J5.

Genome annotation databases

GeneID945752.
GenomeReviewsGene locus JW1178 in contig AP009048_GR.
Gene locus b1189 in contig U00096_GR.
KEGGecj:JW1178.
eco:b1189.

Organism-specific databases

EchoBASEEB1379.
EcoGeneEG11407. dadA.
CMRSearch...

Phylogenomic databases

HOGENOMP0A6J5.
OMAMFQKHAP.

Enzyme and pathway databases

BioCycEcoCyc:DALADEHYDROGA-MON.
MetaCyc:DALADEHYDROGA-MON.

Gene expression databases

GenevestigatorP0A6J5.

Family and domain databases

HAMAPMF_01202.
[Tree]
InterProIPR006076. FAD-dep_OxRdtase.
[Graphical view]
PfamPF01266. DAO. 1 hit.
[Graphical view]
ProDomPD000139. FAD_pyr_redox. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameDADA_ECOLI
AccessionPrimary (citable) accession number: P0A6J5
Secondary accession number(s): P29011
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents