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Protein

Sulfate transporter CysZ

Gene

cysZ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

High affinity, high specificity proton-dependent sulfate transporter, which mediates sulfate uptake. Provides the sulfur source for the cysteine synthesis pathway. Does not transport thiosulfate.1 Publication

Enzyme regulationi

Inhibited by sulfite.1 Publication

Kineticsi

  1. KM=0.72 µM for sulfate1 Publication
  2. KM=1.3 µM for sulfate (in the presence of 5 µM sulfite)1 Publication

    GO - Molecular functioni

    • high-affinity sulfate:proton symporter activity Source: EcoCyc
    • sulfate transmembrane transporter activity Source: EcoCyc

    GO - Biological processi

    • cysteine biosynthetic process Source: EcoCyc
    • sulfate assimilation Source: EcoCyc
    • sulfate transmembrane transport Source: EcoCyc
    Complete GO annotation...

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis, Sulfate transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10003-MONOMER.
    ECOL316407:JW2406-MONOMER.

    Protein family/group databases

    TCDBi2.A.121.1.1. the sulfate transporter (cysz) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sulfate transporter CysZ1 PublicationUniRule annotation
    Gene namesi
    Name:cysZ1 PublicationUniRule annotation
    Ordered Locus Names:b2413, JW2406
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10003. cysZ.

    Subcellular locationi

    • Cell inner membrane UniRule annotation1 Publication; Multi-pass membrane protein UniRule annotation

    Topology

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 3030CytoplasmicCuratedAdd
    BLAST
    Transmembranei31 – 5121HelicalUniRule annotationAdd
    BLAST
    Topological domaini52 – 7423PeriplasmicCuratedAdd
    BLAST
    Transmembranei75 – 9521HelicalUniRule annotationAdd
    BLAST
    Topological domaini96 – 15055CytoplasmicCuratedAdd
    BLAST
    Transmembranei151 – 17121HelicalUniRule annotationAdd
    BLAST
    Topological domaini172 – 22150PeriplasmicCuratedAdd
    BLAST
    Transmembranei222 – 24221HelicalUniRule annotationAdd
    BLAST
    Topological domaini243 – 25311Cytoplasmic1 PublicationAdd
    BLAST

    GO - Cellular componenti

    • integral component of plasma membrane Source: EcoCyc
    • plasma membrane Source: EcoCyc
    Complete GO annotation...

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 253253Sulfate transporter CysZPRO_0000204336Add
    BLAST

    Proteomic databases

    PaxDbiP0A6J3.

    Interactioni

    Protein-protein interaction databases

    BioGridi4259702. 8 interactions.
    STRINGi511145.b2413.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6J3.
    SMRiP0A6J3. Positions 11-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CysZ family.UniRule annotationCurated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG4105EXN. Bacteria.
    COG2981. LUCA.
    HOGENOMiHOG000273989.
    InParanoidiP0A6J3.
    KOiK06203.
    OMAiATLFWVR.
    OrthoDBiEOG657JBT.
    PhylomeDBiP0A6J3.

    Family and domain databases

    HAMAPiMF_00468. CysZ.
    InterProiIPR022985. Sulfate_CysZ.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A6J3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MVSSFTSAPR SGFYYFAQGW KLVSQPGIRR FVILPLLVNI LLMGGAFWWL
    60 70 80 90 100
    FTQLDVWIPT LMSYVPDWLQ WLSYLLWPLA VISVLLVFGY FFSTIANWIA
    110 120 130 140 150
    APFNGLLAEQ LEARLTGATP PDTGIFGIMK DVPRIMKREW QKFAWYLPRA
    160 170 180 190 200
    IVLLILYFIP GIGQTVAPVL WFLFSAWMLA IQYCDYPFDN HKVPFKEMRT
    210 220 230 240 250
    ALRTRKITNM QFGALTSLFT MIPLLNLFIM PVAVCGATAM WVDCYRDKHA

    MWR
    Length:253
    Mass (Da):29,305
    Last modified:March 29, 2005 - v1
    Checksum:i057C4908D9FBB453
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75466.1.
    AP009048 Genomic DNA. Translation: BAA16287.2.
    U74650 Genomic DNA. Translation: AAB42060.1.
    M21451 Genomic DNA. Translation: AAA23653.1.
    X12615 Genomic DNA. Translation: CAA31136.1.
    PIRiD65015. BVECCZ.
    RefSeqiNP_416908.1. NC_000913.3.
    WP_000254839.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75466; AAC75466; b2413.
    BAA16287; BAA16287; BAA16287.
    GeneIDi946875.
    KEGGiecj:JW2406.
    eco:b2413.
    PATRICi32120209. VBIEscCol129921_2507.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U00096 Genomic DNA. Translation: AAC75466.1.
    AP009048 Genomic DNA. Translation: BAA16287.2.
    U74650 Genomic DNA. Translation: AAB42060.1.
    M21451 Genomic DNA. Translation: AAA23653.1.
    X12615 Genomic DNA. Translation: CAA31136.1.
    PIRiD65015. BVECCZ.
    RefSeqiNP_416908.1. NC_000913.3.
    WP_000254839.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP0A6J3.
    SMRiP0A6J3. Positions 11-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4259702. 8 interactions.
    STRINGi511145.b2413.

    Protein family/group databases

    TCDBi2.A.121.1.1. the sulfate transporter (cysz) family.

    Proteomic databases

    PaxDbiP0A6J3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75466; AAC75466; b2413.
    BAA16287; BAA16287; BAA16287.
    GeneIDi946875.
    KEGGiecj:JW2406.
    eco:b2413.
    PATRICi32120209. VBIEscCol129921_2507.

    Organism-specific databases

    EchoBASEiEB0003.
    EcoGeneiEG10003. cysZ.

    Phylogenomic databases

    eggNOGiENOG4105EXN. Bacteria.
    COG2981. LUCA.
    HOGENOMiHOG000273989.
    InParanoidiP0A6J3.
    KOiK06203.
    OMAiATLFWVR.
    OrthoDBiEOG657JBT.
    PhylomeDBiP0A6J3.

    Enzyme and pathway databases

    BioCyciEcoCyc:EG10003-MONOMER.
    ECOL316407:JW2406-MONOMER.

    Miscellaneous databases

    PROiP0A6J3.

    Family and domain databases

    HAMAPiMF_00468. CysZ.
    InterProiIPR022985. Sulfate_CysZ.
    [Graphical view]
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Direct binding of FtsZ to ZipA, an essential component of the septal ring structure that mediates cell division in E. coli."
      Hale C.A., de Boer P.A.J.
      Cell 88:175-185(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-58.
      Strain: PB103.
    5. "DNA sequences of the cysK regions of Salmonella typhimurium and Escherichia coli and linkage of the cysK regions to ptsH."
      Byrne C.R., Monroe R.S., Ward K.A., Kredich N.M.
      J. Bacteriol. 170:3150-3157(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-253.
      Strain: K12.
    6. "Phylogeny of metabolic pathways: O-acetylserine sulphydrylase A is homologous to the tryptophan synthase beta subunit."
      Levy S., Danchin A.
      Mol. Microbiol. 2:777-783(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-253.
    7. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.
    8. "The Escherichia coli CysZ is a pH dependent sulfate transporter that can be inhibited by sulfite."
      Zhang L., Jiang W., Nan J., Almqvist J., Huang Y.
      Biochim. Biophys. Acta 1838:1809-1816(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Strain: K12.

    Entry informationi

    Entry nameiCYSZ_ECOLI
    AccessioniPrimary (citable) accession number: P0A6J3
    Secondary accession number(s): P12610
    , P76533, P76961, P76962
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: July 6, 2016
    This is version 84 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.