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Protein

Adenylyl-sulfate kinase

Gene

cysC

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of activated sulfate.By similarity

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei109 – 1091Phosphoserine intermediate

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi35 – 428ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-3572-MONOMER.
ECOO157:CYSC-MONOMER.
UniPathwayiUPA00140; UER00205.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
APS kinase
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenosine-5'-phosphosulfate kinase
Gene namesi
Name:cysC
Ordered Locus Names:Z4058, ECs3604
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 201200Adenylyl-sulfate kinasePRO_0000105910Add
BLAST

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi155864.Z4058.

Structurei

3D structure databases

ProteinModelPortaliP0A6J2.
SMRiP0A6J2. Positions 26-179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APS kinase family.Curated

Phylogenomic databases

eggNOGiENOG4108I67. Bacteria.
COG0529. LUCA.
HOGENOMiHOG000228204.
KOiK00860.
OMAiNIKWHNL.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6J2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALHDENVVW HSHPVTVQQR ELHHGHRGVV LWFTGLSGSG KSTVAGALEE
60 70 80 90 100
ALHKLGVSTY LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG
110 120 130 140 150
LVVLTAFISP HRAERQMVRE RVGEGRFIEV FVDTPLAICE ARDPKGLYKK
160 170 180 190 200
ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ LVTNLVQQLL DLLRQNDIIR

S
Length:201
Mass (Da):22,321
Last modified:January 23, 2007 - v2
Checksum:i11E15BB8F9D2FD4B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti134 – 1341T → Q (PubMed:11206551).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57857.1.
BA000007 Genomic DNA. Translation: BAB37027.1.
PIRiD91079.
E85924.
RefSeqiNP_311631.1. NC_002695.1.
WP_001173673.1. NZ_LPWC01000044.1.

Genome annotation databases

EnsemblBacteriaiAAG57857; AAG57857; Z4058.
BAB37027; BAB37027; BAB37027.
GeneIDi914674.
KEGGiece:Z4058.
ecs:ECs3604.
PATRICi18356681. VBIEscCol44059_3523.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57857.1.
BA000007 Genomic DNA. Translation: BAB37027.1.
PIRiD91079.
E85924.
RefSeqiNP_311631.1. NC_002695.1.
WP_001173673.1. NZ_LPWC01000044.1.

3D structure databases

ProteinModelPortaliP0A6J2.
SMRiP0A6J2. Positions 26-179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z4058.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57857; AAG57857; Z4058.
BAB37027; BAB37027; BAB37027.
GeneIDi914674.
KEGGiece:Z4058.
ecs:ECs3604.
PATRICi18356681. VBIEscCol44059_3523.

Phylogenomic databases

eggNOGiENOG4108I67. Bacteria.
COG0529. LUCA.
HOGENOMiHOG000228204.
KOiK00860.
OMAiNIKWHNL.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00205.
BioCyciECOL386585:GJFA-3572-MONOMER.
ECOO157:CYSC-MONOMER.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCYSC_ECO57
AccessioniPrimary (citable) accession number: P0A6J2
Secondary accession number(s): P23846
, P78105, Q59376, Q59389
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 76 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.