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Protein

Adenylyl-sulfate kinase

Gene

cysC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of activated sulfate.

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase subunit 1 (cysN), Sulfate adenylyltransferase subunit 2 (cysD)
  2. Adenylyl-sulfate kinase (cysC)
  3. Phosphoadenosine phosphosulfate reductase (cysH)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei109Phosphoserine intermediate1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi35 – 42ATPBy similarity8

GO - Molecular functioni

  • adenylylsulfate kinase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: EcoCyc

GO - Biological processi

  • hydrogen sulfide biosynthetic process Source: UniProtKB-UniPathway
  • phosphorylation Source: EcoCyc
  • sulfate assimilation Source: InterPro

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ADENYLYLSULFKIN-MONOMER
MetaCyc:ADENYLYLSULFKIN-MONOMER
UniPathwayiUPA00140; UER00205

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
APS kinase
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenosine-5'-phosphosulfate kinase
Gene namesi
Name:cysC
Ordered Locus Names:b2750, JW2720
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10185 cysC

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001059092 – 201Adenylyl-sulfate kinaseAdd BLAST200

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP0A6J1
PRIDEiP0A6J1

Interactioni

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263423, 19 interactors
IntActiP0A6J1, 11 interactors
STRINGi316385.ECDH10B_2918

Structurei

3D structure databases

ProteinModelPortaliP0A6J1
SMRiP0A6J1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APS kinase family.Curated

Phylogenomic databases

eggNOGiENOG4107UCJ Bacteria
COG0529 LUCA
HOGENOMiHOG000228204
InParanoidiP0A6J1
KOiK00860
OMAiVSPFRAD
PhylomeDBiP0A6J1

Family and domain databases

CDDicd02027 APSK, 1 hit
HAMAPiMF_00065 Adenylyl_sulf_kinase, 1 hit
InterProiView protein in InterPro
IPR002891 APS_kinase
IPR027417 P-loop_NTPase
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00455 apsK, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A6J1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALHDENVVW HSHPVTVQQR ELHHGHRGVV LWFTGLSGSG KSTVAGALEE
60 70 80 90 100
ALHKLGVSTY LLDGDNVRHG LCSDLGFSDA DRKENIRRVG EVANLMVEAG
110 120 130 140 150
LVVLTAFISP HRAERQMVRE RVGEGRFIEV FVDTPLAICE ARDPKGLYKK
160 170 180 190 200
ARAGELRNFT GIDSVYEAPE SAEIHLNGEQ LVTNLVQQLL DLLRQNDIIR

S
Length:201
Mass (Da):22,321
Last modified:January 23, 2007 - v2
Checksum:i11E15BB8F9D2FD4B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti134T → Q in AAA23647 (PubMed:1316900).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74586 Genomic DNA Translation: AAA23647.1
M86936 Genomic DNA Translation: AAA23503.1
U29579 Genomic DNA Translation: AAA69260.1
U00096 Genomic DNA Translation: AAC75792.1
AP009048 Genomic DNA Translation: BAE76827.1
PIRiB65056
RefSeqiNP_417230.1, NC_000913.3
WP_001173673.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75792; AAC75792; b2750
BAE76827; BAE76827; BAE76827
GeneIDi947221
KEGGiecj:JW2720
eco:b2750
PATRICifig|1411691.4.peg.3990

Similar proteinsi

Entry informationi

Entry nameiCYSC_ECOLI
AccessioniPrimary (citable) accession number: P0A6J1
Secondary accession number(s): P23846
, P78105, Q2MA79, Q59376, Q59389
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 100 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health