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Protein

Dephospho-CoA kinase

Gene

coaE

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A.

Catalytic activityi

ATP + 3'-dephospho-CoA = ADP + CoA.

pH dependencei

Optimum pH is 8.5.

Pathwayi: coenzyme A biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes CoA from (R)-pantothenate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Pantothenate kinase (coaA)
  2. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  3. Coenzyme A biosynthesis bifunctional protein CoaBC (coaBC)
  4. Phosphopantetheine adenylyltransferase (coaD)
  5. Dephospho-CoA kinase (coaE)
This subpathway is part of the pathway coenzyme A biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CoA from (R)-pantothenate, the pathway coenzyme A biosynthesis and in Cofactor biosynthesis.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi9 – 168ATPSequence analysis

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • dephospho-CoA kinase activity Source: EcoCyc

GO - Biological processi

  • coenzyme A biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Coenzyme A biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG12312-MONOMER.
ECOL316407:JW0100-MONOMER.
MetaCyc:EG12312-MONOMER.
BRENDAi2.7.1.24. 2026.
SABIO-RKP0A6I9.
UniPathwayiUPA00241; UER00356.

Names & Taxonomyi

Protein namesi
Recommended name:
Dephospho-CoA kinase (EC:2.7.1.24)
Alternative name(s):
Dephosphocoenzyme A kinase
Gene namesi
Name:coaE
Synonyms:yacE
Ordered Locus Names:b0103, JW0100
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG12312. coaE.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 206206Dephospho-CoA kinasePRO_0000172940Add
BLAST

Proteomic databases

PaxDbiP0A6I9.
PRIDEiP0A6I9.

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

BioGridi4261116. 230 interactions.
DIPiDIP-47935N.
IntActiP0A6I9. 1 interaction.
STRINGi511145.b0103.

Structurei

Secondary structure

1
206
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 86Combined sources
Helixi15 – 2410Combined sources
Beta strandi28 – 314Combined sources
Helixi32 – 387Combined sources
Helixi46 – 549Combined sources
Helixi56 – 583Combined sources
Beta strandi63 – 653Combined sources
Helixi67 – 759Combined sources
Helixi78 – 10225Combined sources
Beta strandi105 – 1117Combined sources
Turni113 – 1197Combined sources
Helixi120 – 1234Combined sources
Beta strandi125 – 1317Combined sources
Helixi134 – 14512Combined sources
Helixi149 – 15810Combined sources
Helixi162 – 1687Combined sources
Beta strandi170 – 1745Combined sources
Helixi179 – 1813Combined sources
Helixi182 – 19918Combined sources
Turni200 – 2023Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N3BX-ray1.80A/B/C1-206[»]
1T3HX-ray2.50A/B/C1-206[»]
1VHLX-ray1.65A/B/C2-206[»]
1VHTX-ray1.59A/B/C2-206[»]
1VIYX-ray1.89A/B/C2-206[»]
ProteinModelPortaliP0A6I9.
SMRiP0A6I9. Positions 1-206.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6I9.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 200197DPCKAdd
BLAST

Sequence similaritiesi

Belongs to the CoaE family.Curated

Phylogenomic databases

eggNOGiENOG4108ZQD. Bacteria.
COG0237. LUCA.
HOGENOMiHOG000020769.
InParanoidiP0A6I9.
KOiK00859.
OMAiGGKKRYP.
OrthoDBiEOG6HTP3H.
PhylomeDBiP0A6I9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00376. Dephospho_CoA_kinase.
InterProiIPR001977. Depp_CoAkinase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01121. CoaE. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00152. TIGR00152. 1 hit.
PROSITEiPS51219. DPCK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRYIVALTGG IGSGKSTVAN AFADLGINVI DADIIARQVV EPGAPALHAI
60 70 80 90 100
ADHFGANMIA ADGTLQRRAL RERIFANPEE KNWLNALLHP LIQQETQHQI
110 120 130 140 150
QQATSPYVLW VVPLLVENSL YKKANRVLVV DVSPETQLKR TMQRDDVTRE
160 170 180 190 200
HVEQILAAQA TREARLAVAD DVIDNNGAPD AIASDVARLH AHYLQLASQF

VSQEKP
Length:206
Mass (Da):22,622
Last modified:March 29, 2005 - v1
Checksum:i41C69353B4EFF871
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti72 – 743ERI → DGL (PubMed:8202364).Curated
Sequence conflicti86 – 861A → V (PubMed:8202364).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73214.1.
AP009048 Genomic DNA. Translation: BAE76040.1.
PIRiG64732.
RefSeqiNP_414645.1. NC_000913.3.
WP_001269520.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73214; AAC73214; b0103.
BAE76040; BAE76040; BAE76040.
GeneIDi949060.
KEGGiecj:JW0100.
eco:b0103.
PATRICi32115309. VBIEscCol129921_0106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC73214.1.
AP009048 Genomic DNA. Translation: BAE76040.1.
PIRiG64732.
RefSeqiNP_414645.1. NC_000913.3.
WP_001269520.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N3BX-ray1.80A/B/C1-206[»]
1T3HX-ray2.50A/B/C1-206[»]
1VHLX-ray1.65A/B/C2-206[»]
1VHTX-ray1.59A/B/C2-206[»]
1VIYX-ray1.89A/B/C2-206[»]
ProteinModelPortaliP0A6I9.
SMRiP0A6I9. Positions 1-206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261116. 230 interactions.
DIPiDIP-47935N.
IntActiP0A6I9. 1 interaction.
STRINGi511145.b0103.

Proteomic databases

PaxDbiP0A6I9.
PRIDEiP0A6I9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73214; AAC73214; b0103.
BAE76040; BAE76040; BAE76040.
GeneIDi949060.
KEGGiecj:JW0100.
eco:b0103.
PATRICi32115309. VBIEscCol129921_0106.

Organism-specific databases

EchoBASEiEB2218.
EcoGeneiEG12312. coaE.

Phylogenomic databases

eggNOGiENOG4108ZQD. Bacteria.
COG0237. LUCA.
HOGENOMiHOG000020769.
InParanoidiP0A6I9.
KOiK00859.
OMAiGGKKRYP.
OrthoDBiEOG6HTP3H.
PhylomeDBiP0A6I9.

Enzyme and pathway databases

UniPathwayiUPA00241; UER00356.
BioCyciEcoCyc:EG12312-MONOMER.
ECOL316407:JW0100-MONOMER.
MetaCyc:EG12312-MONOMER.
BRENDAi2.7.1.24. 2026.
SABIO-RKP0A6I9.

Miscellaneous databases

EvolutionaryTraceiP0A6I9.
PROiP0A6I9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00376. Dephospho_CoA_kinase.
InterProiIPR001977. Depp_CoAkinase.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF01121. CoaE. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00152. TIGR00152. 1 hit.
PROSITEiPS51219. DPCK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region."
    Fujita N., Mori H., Yura T., Ishihama A.
    Nucleic Acids Res. 22:1637-1639(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Identification of yacE (coaE) as the structural gene for dephosphocoenzyme A kinase in Escherichia coli K-12."
    Mishra P.K., Park P.K., Drueckhammer D.G.
    J. Bacteriol. 183:2774-2778(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-12, CHARACTERIZATION.
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
    Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
    Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: B / BL21.

Entry informationi

Entry nameiCOAE_ECOLI
AccessioniPrimary (citable) accession number: P0A6I9
Secondary accession number(s): P36679, P75646, Q2MCG6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: January 20, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.