P0A6I3 (COAA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pantothenate kinase
EC=2.7.1.33

Alternative name(s):
Pantothenic acid kinase
Rts protein

Gene names

Name:	coaA
Synonyms:	panK, rts
Ordered Locus Names:	b3974, JW3942

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	316 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + (R)-pantothenate = ADP + (R)-4'-phosphopantothenate. HAMAP-Rule MF_00215
Enzyme regulation	Regulated by feedback inhibition by CoA and its thioesters. HAMAP-Rule MF_00215
Pathway	Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)-pantothenate: step 1/5. HAMAP-Rule MF_00215
Subcellular location	Cytoplasm Probable HAMAP-Rule MF_00215.
Sequence similarities	Belongs to the prokaryotic pantothenate kinase family.
Sequence caution	The sequence AAA23591.1 differs from that shown. Reason: Erroneous initiation. The sequence AAA76838.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Coenzyme A biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	coenzyme A biosynthetic process Inferred from mutant phenotype PubMed 2824448. Source: EcoCyc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from direct assay PubMed 7929447. Source: EcoCyc pantothenate kinase activity Inferred from direct assay PubMed 7929447. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 316

316

Pantothenate kinase HAMAP-Rule MF_00215

PRO_0000194426

Regions

Nucleotide binding

95 – 102

8

ATP Potential

Secondary structure

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316

Helix Strand Turn

Details...

Beta strand

11 – 16

6

Helix

17 – 22

6

Helix

33 – 42

10

Beta strand

44 – 46

3

Helix

48 – 53

6

Helix

55 – 80

26

Beta strand

89 – 95

7

Helix

101 – 112

12

Beta strand

115 – 117

3

Beta strand

121 – 125

5

Helix

126 – 129

4

Helix

133 – 139

7

Helix

148 – 150

3

Helix

153 – 163

11

Turn

164 – 166

3

Beta strand

170 – 172

3

Turn

177 – 180

4

Beta strand

188 – 190

3

Beta strand

195 – 199

5

Turn

201 – 204

4

Helix

207 – 209

3

Helix

219 – 222

4

Beta strand

224 – 230

7

Helix

233 – 249

17

Turn

250 – 253

4

Helix

260 – 263

4

Helix

267 – 280

14

Helix

282 – 288

7

Helix

291 – 296

6

Beta strand

298 – 303

6

Helix

305 – 307

3

Beta strand

309 – 315

7

Sequences

	Sequence		Length	Mass (Da)
	P0A6I3 [UniParc]. Last modified March 29, 2005. Version 1. Checksum: DDDC1922C5C52A70	FASTA	316	36,360
	10 20 30 40 50 60 MSIKEQTLMT PYLQFDRNQW AALRDSVPMT LSEDEIARLK GINEDLSLEE VAEIYLPLSR 70 80 90 100 110 120 LLNFYISSNL RRQAVLEQFL GTNGQRIPYI ISIAGSVAVG KSTTARVLQA LLSRWPEHRR 130 140 150 160 170 180 VELITTDGFL HPNQVLKERG LMKKKGFPES YDMHRLVKFV SDLKSGVPNV TAPVYSHLIY 190 200 210 220 230 240 DVIPDGDKTV VQPDILILEG LNVLQSGMDY PHDPHHVFVS DFVDFSIYVD APEDLLQTWY 250 260 270 280 290 300 INRFLKFREG AFTDPDSYFH NYAKLTKEEA IKTAMTLWKE INWLNLKQNI LPTRERASLI 310 LTKSANHAVE EVRLRK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, sequencing, and expression of the pantothenate kinase (coaA) gene of Escherichia coli." Song W.-J., Jackowski S. J. Bacteriol. 174:6411-6417(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"coaA and rts are allelic and located at kilobase 3532 on the Escherichia coli physical map." Song W.-J., Jackowski S. J. Bacteriol. 174:1705-1706(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The nucleotide sequence of the Escherichia coli rts gene." Flamm J.A., Friesen J.D., Otsuka A.J. Gene 74:555-558(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M36321 Genomic DNA. Translation: AAA76838.1. Different initiation.
M90071 Genomic DNA. Translation: AAA23590.1.
M90071 Genomic DNA. Translation: AAA23591.1. Different initiation.
U00006 Genomic DNA. Translation: AAC43076.1.
U00096 Genomic DNA. Translation: AAC76952.1.
AP009048 Genomic DNA. Translation: BAE77341.1.

PIR

BVECRS. A45727.

RefSeq

NP_418405.1. NC_000913.2.
YP_491482.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1ESM	X-ray	2.50	A/B/C/D	1-316	[»]
1ESN	X-ray	2.60	A/B/C/D	1-316	[»]
1SQ5	X-ray	2.20	A/B/C/D	9-316	[»]

ProteinModelPortal

P0A6I3.

SMR

P0A6I3. Positions 6-316.

ModBase

Search...

Protein-protein interaction databases

IntAct

P0A6I3. 2 interactions.

STRING

511145.b3974.

Proteomic databases

PaxDb

P0A6I3.

PRIDE

P0A6I3.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76952; AAC76952; b3974.
BAE77341; BAE77341; BAE77341.

GeneID

12934389.
948479.

KEGG

ecj:Y75_p3218.
eco:b3974.

PATRIC

32123469. VBIEscCol129921_4091.

Organism-specific databases

EchoBASE

EB0915.

EcoGene

EG10922. coaA.

Phylogenomic databases

eggNOG

COG1072.

HOGENOM

HOG000248571.

KO

K00867.

OMA

WYIDRFL.

ProtClustDB

PRK05439.

Enzyme and pathway databases

BioCyc

EcoCyc:PANTOTHENATE-KIN-MONOMER.
ECOL316407:JW3942-MONOMER.
MetaCyc:PANTOTHENATE-KIN-MONOMER.

SABIO-RK

P0A6I3.

UniPathway

UPA00241; UER00352.

Gene expression databases

Genevestigator

P0A6I3.

Family and domain databases

HAMAP

MF_00215. Pantothen_kinase_1.

InterPro

IPR004566. PanK_bact.
IPR006083. PRK/URK.
[Graphical view]

PANTHER

PTHR10285:SF7. PTHR10285:SF7. 1 hit.

Pfam

PF00485. PRK. 1 hit.
[Graphical view]

PIRSF

PIRSF000545. Pantothenate_kin. 1 hit.

TIGRFAMs

TIGR00554. panK_bact. 1 hit.

ProtoNet

Search...

Other

ChEMBL

CHEMBL5092.

EvolutionaryTrace

P0A6I3.

Entry information

Entry name

COAA_ECOLI

Accession

Primary (citable) accession number: P0A6I3
Secondary accession number(s): P15044, Q2M8R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program