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Protein

Cytidylate kinase

Gene

cmk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

ATP, dATP, and GTP are equally effective as phosphate donors. CMP and dCMP are the best phosphate acceptors.2 Publications

Catalytic activityi

ATP + (d)CMP = ADP + (d)CDP.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 20ATPBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-HAMAP
  • cytidylate kinase activity Source: EcoCyc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CMPKI-MONOMER.
ECOL316407:JW0893-MONOMER.
MetaCyc:CMPKI-MONOMER.
BRENDAi2.7.4.25. 2026.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytidylate kinase (EC:2.7.4.25)
Short name:
CK
Alternative name(s):
Cytidine monophosphate kinase
Short name:
CMP kinase
Protein MssA
p25
Gene namesi
Name:cmk
Synonyms:mssA, ycaF, ycaG
Ordered Locus Names:b0910, JW0893
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11265. cmk.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001319131 – 227Cytidylate kinaseAdd BLAST227

Proteomic databases

PaxDbiP0A6I0.
PRIDEiP0A6I0.

Interactioni

Protein-protein interaction databases

BioGridi4260009. 256 interactors.
IntActiP0A6I0. 6 interactors.
STRINGi511145.b0910.

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi18 – 29Combined sources12
Beta strandi32 – 35Combined sources4
Helixi36 – 50Combined sources15
Helixi57 – 65Combined sources9
Beta strandi69 – 74Combined sources6
Beta strandi77 – 82Combined sources6
Helixi88 – 91Combined sources4
Helixi94 – 103Combined sources10
Helixi107 – 118Combined sources12
Beta strandi126 – 132Combined sources7
Helixi133 – 137Combined sources5
Beta strandi142 – 148Combined sources7
Helixi151 – 165Combined sources15
Helixi171 – 178Combined sources8
Beta strandi189 – 191Combined sources3
Beta strandi200 – 203Combined sources4
Turni204 – 206Combined sources3
Helixi209 – 224Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CKEX-ray1.75A1-227[»]
1KDOX-ray1.90A/B1-227[»]
1KDPX-ray2.30A/B1-227[»]
1KDRX-ray2.25A/B1-227[»]
1KDTX-ray1.95A/B1-227[»]
2CMKX-ray2.00A1-227[»]
2FEMX-ray1.90A1-227[»]
2FEOX-ray2.80A1-227[»]
ProteinModelPortaliP0A6I0.
SMRiP0A6I0.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6I0.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CAT. Bacteria.
COG0283. LUCA.
HOGENOMiHOG000242849.
InParanoidiP0A6I0.
KOiK00945.
OMAiVRARRRY.
PhylomeDBiP0A6I0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00238. Cytidyl_kinase_type1. 1 hit.
InterProiIPR003136. Cytidylate_kin.
IPR011994. Cytidylate_kinase_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02224. Cytidylate_kin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00017. cmk. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAIAPVITI DGPSGAGKGT LCKAMAEALQ WHLLDSGAIY RVLALAALHH
60 70 80 90 100
HVDVASEDAL VPLASHLDVR FVSTNGNLEV ILEGEDVSGE IRTQEVANAA
110 120 130 140 150
SQVAAFPRVR EALLRRQRAF RELPGLIADG RDMGTVVFPD APVKIFLDAS
160 170 180 190 200
SEERAHRRML QLQEKGFSVN FERLLAEIKE RDDRDRNRAV APLVPAADAL
210 220
VLDSTTLSIE QVIEKALQYA RQKLALA
Length:227
Mass (Da):24,746
Last modified:March 29, 2005 - v1
Checksum:iB1578B6B06966776
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164E → V in CAA25360 (PubMed:6384724).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00785 Genomic DNA. Translation: CAA25360.1.
X82933 Genomic DNA. Translation: CAA58107.1.
U00096 Genomic DNA. Translation: AAC73996.1.
AP009048 Genomic DNA. Translation: BAA35645.1.
V00352 Genomic DNA. No translation available.
PIRiA04448. QQEC31.
E64830.
RefSeqiNP_415430.1. NC_000913.3.
WP_000125016.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73996; AAC73996; b0910.
BAA35645; BAA35645; BAA35645.
GeneIDi945535.
KEGGiecj:JW0893.
eco:b0910.
PATRICi32117033. VBIEscCol129921_0941.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00785 Genomic DNA. Translation: CAA25360.1.
X82933 Genomic DNA. Translation: CAA58107.1.
U00096 Genomic DNA. Translation: AAC73996.1.
AP009048 Genomic DNA. Translation: BAA35645.1.
V00352 Genomic DNA. No translation available.
PIRiA04448. QQEC31.
E64830.
RefSeqiNP_415430.1. NC_000913.3.
WP_000125016.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CKEX-ray1.75A1-227[»]
1KDOX-ray1.90A/B1-227[»]
1KDPX-ray2.30A/B1-227[»]
1KDRX-ray2.25A/B1-227[»]
1KDTX-ray1.95A/B1-227[»]
2CMKX-ray2.00A1-227[»]
2FEMX-ray1.90A1-227[»]
2FEOX-ray2.80A1-227[»]
ProteinModelPortaliP0A6I0.
SMRiP0A6I0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260009. 256 interactors.
IntActiP0A6I0. 6 interactors.
STRINGi511145.b0910.

Proteomic databases

PaxDbiP0A6I0.
PRIDEiP0A6I0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73996; AAC73996; b0910.
BAA35645; BAA35645; BAA35645.
GeneIDi945535.
KEGGiecj:JW0893.
eco:b0910.
PATRICi32117033. VBIEscCol129921_0941.

Organism-specific databases

EchoBASEiEB1244.
EcoGeneiEG11265. cmk.

Phylogenomic databases

eggNOGiENOG4105CAT. Bacteria.
COG0283. LUCA.
HOGENOMiHOG000242849.
InParanoidiP0A6I0.
KOiK00945.
OMAiVRARRRY.
PhylomeDBiP0A6I0.

Enzyme and pathway databases

BioCyciEcoCyc:CMPKI-MONOMER.
ECOL316407:JW0893-MONOMER.
MetaCyc:CMPKI-MONOMER.
BRENDAi2.7.4.25. 2026.

Miscellaneous databases

EvolutionaryTraceiP0A6I0.
PROiP0A6I0.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00238. Cytidyl_kinase_type1. 1 hit.
InterProiIPR003136. Cytidylate_kin.
IPR011994. Cytidylate_kinase_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF02224. Cytidylate_kin. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00017. cmk. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKCY_ECOLI
AccessioniPrimary (citable) accession number: P0A6I0
Secondary accession number(s): P03823, P23863, P78263
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: November 2, 2016
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.