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Protein

Cytidylate kinase

Gene

cmk

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP, dATP, and GTP are equally effective as phosphate donors. CMP and dCMP are the best phosphate acceptors.2 Publications

Catalytic activityi

ATP + (d)CMP = ADP + (d)CDP.2 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 20ATPBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cytidylate kinase activity Source: EcoCyc
  • pantoate-beta-alanine ligase activity Source: GO_Central

GO - Biological processi

  • nucleobase-containing small molecule interconversion Source: EcoCyc
  • pantothenate biosynthetic process from valine Source: GO_Central
  • response to X-ray Source: EcoCyc

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:CMPKI-MONOMER
MetaCyc:CMPKI-MONOMER
BRENDAi2.7.4.25 2026

Names & Taxonomyi

Protein namesi
Recommended name:
Cytidylate kinase (EC:2.7.4.25)
Short name:
CK
Alternative name(s):
Cytidine monophosphate kinase
Short name:
CMP kinase
Protein MssA
p25
Gene namesi
Name:cmk
Synonyms:mssA, ycaF, ycaG
Ordered Locus Names:b0910, JW0893
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11265 cmk

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB04555 Cytidine-5'-Diphosphate
DB03403 Cytidine-5'-Monophosphate
DB02456 Cytosine Arabinose-5'-Phosphate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001319131 – 227Cytidylate kinaseAdd BLAST227

Proteomic databases

PaxDbiP0A6I0
PRIDEiP0A6I0

Interactioni

Protein-protein interaction databases

BioGridi4260009, 269 interactors
IntActiP0A6I0, 6 interactors
STRINGi316385.ECDH10B_0980

Structurei

Secondary structure

1227
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi18 – 29Combined sources12
Beta strandi32 – 35Combined sources4
Helixi36 – 50Combined sources15
Helixi57 – 65Combined sources9
Beta strandi69 – 74Combined sources6
Beta strandi77 – 82Combined sources6
Helixi88 – 91Combined sources4
Helixi94 – 103Combined sources10
Helixi107 – 118Combined sources12
Beta strandi126 – 132Combined sources7
Helixi133 – 137Combined sources5
Beta strandi142 – 148Combined sources7
Helixi151 – 165Combined sources15
Helixi171 – 178Combined sources8
Beta strandi189 – 191Combined sources3
Beta strandi200 – 203Combined sources4
Turni204 – 206Combined sources3
Helixi209 – 224Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CKEX-ray1.75A1-227[»]
1KDOX-ray1.90A/B1-227[»]
1KDPX-ray2.30A/B1-227[»]
1KDRX-ray2.25A/B1-227[»]
1KDTX-ray1.95A/B1-227[»]
2CMKX-ray2.00A1-227[»]
2FEMX-ray1.90A1-227[»]
2FEOX-ray2.80A1-227[»]
ProteinModelPortaliP0A6I0
SMRiP0A6I0
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP0A6I0

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CAT Bacteria
COG0283 LUCA
HOGENOMiHOG000242849
InParanoidiP0A6I0
KOiK00945
OMAiLKIFMTA
PhylomeDBiP0A6I0

Family and domain databases

HAMAPiMF_00238 Cytidyl_kinase_type1, 1 hit
InterProiView protein in InterPro
IPR003136 Cytidylate_kin
IPR011994 Cytidylate_kinase_dom
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF02224 Cytidylate_kin, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00017 cmk, 1 hit

Sequencei

Sequence statusi: Complete.

P0A6I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAIAPVITI DGPSGAGKGT LCKAMAEALQ WHLLDSGAIY RVLALAALHH
60 70 80 90 100
HVDVASEDAL VPLASHLDVR FVSTNGNLEV ILEGEDVSGE IRTQEVANAA
110 120 130 140 150
SQVAAFPRVR EALLRRQRAF RELPGLIADG RDMGTVVFPD APVKIFLDAS
160 170 180 190 200
SEERAHRRML QLQEKGFSVN FERLLAEIKE RDDRDRNRAV APLVPAADAL
210 220
VLDSTTLSIE QVIEKALQYA RQKLALA
Length:227
Mass (Da):24,746
Last modified:March 29, 2005 - v1
Checksum:iB1578B6B06966776
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti164E → V in CAA25360 (PubMed:6384724).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X00785 Genomic DNA Translation: CAA25360.1
X82933 Genomic DNA Translation: CAA58107.1
U00096 Genomic DNA Translation: AAC73996.1
AP009048 Genomic DNA Translation: BAA35645.1
V00352 Genomic DNA No translation available.
PIRiA04448 QQEC31
E64830
RefSeqiNP_415430.1, NC_000913.3
WP_000125016.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73996; AAC73996; b0910
BAA35645; BAA35645; BAA35645
GeneIDi945535
KEGGiecj:JW0893
eco:b0910
PATRICifig|1411691.4.peg.1366

Similar proteinsi

Entry informationi

Entry nameiKCY_ECOLI
AccessioniPrimary (citable) accession number: P0A6I0
Secondary accession number(s): P03823, P23863, P78263
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: March 29, 2005
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
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Main funding by: National Institutes of Health