Cardiolipin synthase - Escherichia coli (strain K12)

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P0A6H8 (CLS_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 70. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cardiolipin synthase
Short name=CL synthase
EC=2.7.8.-

Gene names

Name:	cls
Synonyms:	nov, yciJ
Ordered Locus Names:	b1249, JW1241

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	486 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol. Affects resistance to the gyrase inhibitor novobiocin. HAMAP-Rule MF_00190
Catalytic activity	2 Phosphatidylglycerol = diphosphatidylglycerol + glycerol. HAMAP-Rule MF_00190
Subunit structure	Monomer.
Subcellular location	Cell inner membrane; Multi-pass membrane protein Ref.12.
Post-translational modification	Seems to be post-translationally processed from a 53-55 kDa form to a 45-46 kDa form. HAMAP-Rule MF_00190
Sequence similarities	Belongs to the phospholipase D family. Cardiolipin synthase subfamily. Contains 2 PLD phosphodiesterase domains.

Ontologies

Keywords
Biological process	Lipid biosynthesis Lipid metabolism Phospholipid biosynthesis Phospholipid metabolism
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiolipin biosynthetic process Inferred from mutant phenotype PubMed 2982784 PubMed 353047. Source: EcoCyc
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW membrane Inferred from direct assay PubMed 353047. Source: EcoCyc plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cardiolipin synthase activity Inferred from direct assay Ref.10 PubMed 7918616. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 486

486

Cardiolipin synthase HAMAP-Rule MF_00190

PRO_0000201253

Regions

Transmembrane

3 – 23

Helical; Potential

Transmembrane

38 – 58

Helical; Potential

Domain

219 – 246

PLD phosphodiesterase 1

Domain

399 – 426

PLD phosphodiesterase 2

Sites

Active site

224

Potential

Active site

226

Potential

Active site

231

Potential

Active site

404

Potential

Active site

406

Potential

Active site

411

Potential

Natural variations

Natural variant

113

I → S in strain: ECOR 60.

Natural variant

283

E → G in strain: ECOR 50.

Natural variant

378

G → S in strain: ECOR 16.

Natural variant

426

S → R in strain: ECOR 46.

Sequences

Sequence

Length

Mass (Da)

Tools

P0A6H8 [UniParc].

Last modified March 29, 2005. Version 1.
Checksum: C8BDD69AA5AA37DB
Show »

FASTA

486

54,822

        10         20         30         40         50         60 
MTTVYTLVSW LAILGYWLLI AGVTLRILMK RRAVPSAMAW LLIIYILPLV GIIAYLAVGE 

        70         80         90        100        110        120 
LHLGKRRAER ARAMWPSTAK WLNDLKACKH IFAEENSSVA APLFKLCERR QGIAGVKGNQ 

       130        140        150        160        170        180 
LQLMTESDDV MQALIRDIQL ARHNIEMVFY IWQPGGMADQ VAESLMAAAR RGIHCRLMLD 

       190        200        210        220        230        240 
SAGSVAFFRS PWPELMRNAG IEVVEALKVN LMRVFLRRMD LRQHRKMIMI DNYIAYTGSM 

       250        260        270        280        290        300 
NMVDPRYFKQ DAGVGQWIDL MARMEGPIAT AMGIIYSCDW EIETGKRILP PPPDVNIMPF 

       310        320        330        340        350        360 
EQASGHTIHT IASGPGFPED LIHQALLTAA YSAREYLIMT TPYFVPSDDL LHAICTAAQR 

       370        380        390        400        410        420 
GVDVSIILPR KNDSMLVGWA SRAFFTELLA AGVKIYQFEG GLLHTKSVLV DGELSLVGTV 

       430        440        450        460        470        480 
NLDMRSLWLN FEITLAIDDK GFGADLAAVQ DDYISRSRLL DARLWLKRPL WQRVAERLFY 


FFSPLL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An Escherichia coli homologue of eukaryotic potassium channel proteins." Milkman R. Proc. Natl. Acad. Sci. U.S.A. 91:3510-3514(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"Nucleotide sequence, mutational analysis, transcriptional start site, and product analysis of nov, the gene which affects Escherichia coli K-12 resistance to the gyrase inhibitor novobiocin." Ivanisevic R., Milic M., Ajdic D., Rakonjac J.V., Savic D.J. J. Bacteriol. 177:1766-1771(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]	Shibuya I., Asami Y., Uetake N., Ohta A., Matsuzaki H., Matsumoto K. Submitted (NOV-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / C600 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222.
[4]	Xia W., Dowhan W. Submitted (OCT-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[5]	Milkman R. Submitted (APR-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 and Various ECOR strains.
[6]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[8]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[9]	"Identity of the Escherichia coli cls and nov genes." Tropp B.E., Ragolia L., Xia W., Dowhan W., Milkman R., Rudd K.E., Ivanisevic R., Savic D.J. J. Bacteriol. 177:5155-5157(1995) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF CLS AS NOV.
[10]	"Amplification and substantial purification of cardiolipin synthase of Escherichia coli." Hiraoka S., Nukui K., Uetake N., Ohta A., Shibuya I. J. Biochem. 110:443-449(1991) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.
[11]	"Cardiolipin synthase from Escherichia coli." Tropp B.E. Biochim. Biophys. Acta 1348:192-200(1997) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[12]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L12044 Genomic DNA. Translation: AAA66948.1. U01911 Genomic DNA. Translation: AAA82872.1. D38779 Genomic DNA. Translation: BAA07636.1. U15986 Genomic DNA. Translation: AAA53368.1. U24206 Genomic DNA. Translation: AAB60160.1. U24195 Genomic DNA. Translation: AAB60072.1. U24196 Genomic DNA. Translation: AAB60080.1. U24197 Genomic DNA. Translation: AAB60088.1. U24198 Genomic DNA. Translation: AAB60096.1. U24199 Genomic DNA. Translation: AAB60104.1. U24200 Genomic DNA. Translation: AAB60112.1. U24201 Genomic DNA. Translation: AAB60120.1. U24202 Genomic DNA. Translation: AAB60128.1. U24203 Genomic DNA. Translation: AAB60136.1. U24204 Genomic DNA. Translation: AAB60144.1. U24205 Genomic DNA. Translation: AAB60152.1. U00096 Genomic DNA. Translation: AAC74331.1. AP009048 Genomic DNA. Translation: BAA14781.1.
PIR	A56145.
RefSeq	NP_415765.1. NC_000913.2. YP_489517.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P0A6H8.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1249.
Proteomic databases
PaxDb	P0A6H8.
PRIDE	P0A6H8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74331; AAC74331; b1249. BAA14781; BAA14781; BAA14781.
GeneID	12931118. 945821.
KEGG	ecj:Y75_p1223. eco:b1249.
PATRIC	32117758. VBIEscCol129921_1299.
Organism-specific databases
EchoBASE	EB1565.
EcoGene	EG11608. cls.
Phylogenomic databases
eggNOG	COG1502.
HOGENOM	HOG000077403.
KO	K06131.
OMA	IMPRKND.
ProtClustDB	PRK01642.
Enzyme and pathway databases
BioCyc	EcoCyc:CARDIOLIPSYN-MONOMER. ECOL316407:JW1241-MONOMER. MetaCyc:CARDIOLIPSYN-MONOMER.
Gene expression databases
Genevestigator	P0A6H8.
Family and domain databases
HAMAP	MF_00190. Cardiolipin_synth.
InterPro	IPR022924. Cardiolipin_synthase. IPR001736. PLipase_D/transphosphatidylase. [Graphical view]
Pfam	PF00614. PLDc. 2 hits. [Graphical view]
SMART	SM00155. PLDc. 2 hits. [Graphical view]
TIGRFAMs	TIGR04265. bac_cardiolipin. 1 hit.
PROSITE	PS50035. PLD. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLS_ECOLI

Accession

Primary (citable) accession number: P0A6H8
Secondary accession number(s): P31071

P94740

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2005
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Natural variations

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents