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Protein

Nicotinamide-nucleotide amidohydrolase PncC

Gene

pncC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Has nicotinamidemononucleotide (NMN) aminohydrolase activity, not active on other substrates.1 Publication

Catalytic activityi

Beta-nicotinamide D-ribonucleotide + H2O = beta-nicotinate D-ribonucleotide + NH3.1 Publication

Kineticsi

Kcat is 3.3 s (-1).
  1. KM=6 µM for NMN1 Publication

    pH dependencei

    Optimum pH active over a broad range of pH values.1 Publication

    GO - Molecular functioni

    • nicotinamide-nucleotide amidase activity Source: EcoCyc

    GO - Biological processi

    Keywordsi

    Molecular functionHydrolase
    Biological processPyridine nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:G7409-MONOMER
    MetaCyc:G7409-MONOMER

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinamide-nucleotide amidohydrolase PncC (EC:3.5.1.42)
    Short name:
    NMN amidohydrolase PncC
    Alternative name(s):
    NMN deamidase
    Nicotinamide-nucleotide amidase
    Gene namesi
    Name:pncC
    Synonyms:ygaD
    Ordered Locus Names:b2700, JW2670
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12698 pncC

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001567901 – 165Nicotinamide-nucleotide amidohydrolase PncCAdd BLAST165

    Proteomic databases

    PaxDbiP0A6G3
    PRIDEiP0A6G3

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi4262070, 139 interactors
    DIPiDIP-48189N
    IntActiP0A6G3, 9 interactors
    STRINGi316385.ECDH10B_2868

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6G3
    SMRiP0A6G3
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CinA family. PncC subfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4107TH8 Bacteria
    COG1546 LUCA
    HOGENOMiHOG000242527
    InParanoidiP0A6G3
    KOiK03743
    OMAiVEHALNM
    PhylomeDBiP0A6G3

    Family and domain databases

    Gene3Di3.90.950.20, 1 hit
    InterProiView protein in InterPro
    IPR036653 CinA-like_C
    IPR008136 CinA_C
    PfamiView protein in Pfam
    PF02464 CinA, 1 hit
    SUPFAMiSSF142433 SSF142433, 1 hit
    TIGRFAMsiTIGR00199 PncC_domain, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P0A6G3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTDSELMQLS EQVGQALKAR GATVTTAESC TGGWVAKVIT DIAGSSAWFE
    60 70 80 90 100
    RGFVTYSNEA KAQMIGVREE TLAQHGAVSE PVVVEMAIGA LKAARADYAV
    110 120 130 140 150
    SISGIAGPDG GSEEKPVGTV WFAFATARGE GITRRECFSG DRDAVRRQAT
    160
    AYALQTLWQQ FLQNT
    Length:165
    Mass (Da):17,582
    Last modified:March 29, 2005 - v1
    Checksum:i9B6D41D25D5DE212
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18785 Genomic DNA Translation: AAB60061.1
    U00096 Genomic DNA Translation: AAC75742.1
    AP009048 Genomic DNA Translation: BAA16562.1
    V00328 Genomic DNA No translation available.
    PIRiH65049
    RefSeqiNP_417180.1, NC_000913.3
    WP_000132231.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC75742; AAC75742; b2700
    BAA16562; BAA16562; BAA16562
    GeneIDi947169
    KEGGiecj:JW2670
    eco:b2700
    PATRICifig|1411691.4.peg.4042

    Similar proteinsi

    Entry informationi

    Entry nameiPNCC_ECOLI
    AccessioniPrimary (citable) accession number: P0A6G3
    Secondary accession number(s): P41053
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: March 28, 2018
    This is version 95 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

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