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Protein

Nicotinamide-nucleotide amidohydrolase PncC

Gene

pncC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Has nicotinamidemononucleotide (NMN) aminohydrolase activity, not active on other substrates.1 Publication

Catalytic activityi

Beta-nicotinamide D-ribonucleotide + H2O = beta-nicotinate D-ribonucleotide + NH3.1 Publication

Kineticsi

Kcat is 3.3 s (-1).

  1. KM=6 µM for NMN1 Publication

    pH dependencei

    Optimum pH active over a broad range of pH values.1 Publication

    GO - Molecular functioni

    • nicotinamide-nucleotide amidase activity Source: EcoCyc

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Pyridine nucleotide biosynthesis

    Enzyme and pathway databases

    BioCyciEcoCyc:G7409-MONOMER.
    ECOL316407:JW2670-MONOMER.
    MetaCyc:G7409-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nicotinamide-nucleotide amidohydrolase PncC (EC:3.5.1.42)
    Short name:
    NMN amidohydrolase PncC
    Alternative name(s):
    NMN deamidase
    Nicotinamide-nucleotide amidase
    Gene namesi
    Name:pncC
    Synonyms:ygaD
    Ordered Locus Names:b2700, JW2670
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG12698. pncC.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 165165Nicotinamide-nucleotide amidohydrolase PncCPRO_0000156790Add
    BLAST

    Proteomic databases

    EPDiP0A6G3.
    PaxDbiP0A6G3.
    PRIDEiP0A6G3.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi4262070. 139 interactions.
    DIPiDIP-48189N.
    IntActiP0A6G3. 8 interactions.
    STRINGi511145.b2700.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6G3.
    SMRiP0A6G3. Positions 7-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the CinA family. PncC subfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4107TH8. Bacteria.
    COG1546. LUCA.
    HOGENOMiHOG000242527.
    InParanoidiP0A6G3.
    KOiK03743.
    OMAiTVWFAWA.
    OrthoDBiEOG61S359.
    PhylomeDBiP0A6G3.

    Family and domain databases

    Gene3Di3.90.950.20. 1 hit.
    InterProiIPR008136. CinA_C.
    [Graphical view]
    PfamiPF02464. CinA. 1 hit.
    [Graphical view]
    SUPFAMiSSF142433. SSF142433. 1 hit.
    TIGRFAMsiTIGR00199. PncC_domain. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P0A6G3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTDSELMQLS EQVGQALKAR GATVTTAESC TGGWVAKVIT DIAGSSAWFE
    60 70 80 90 100
    RGFVTYSNEA KAQMIGVREE TLAQHGAVSE PVVVEMAIGA LKAARADYAV
    110 120 130 140 150
    SISGIAGPDG GSEEKPVGTV WFAFATARGE GITRRECFSG DRDAVRRQAT
    160
    AYALQTLWQQ FLQNT
    Length:165
    Mass (Da):17,582
    Last modified:March 29, 2005 - v1
    Checksum:i9B6D41D25D5DE212
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18785 Genomic DNA. Translation: AAB60061.1.
    U00096 Genomic DNA. Translation: AAC75742.1.
    AP009048 Genomic DNA. Translation: BAA16562.1.
    V00328 Genomic DNA. No translation available.
    PIRiH65049.
    RefSeqiNP_417180.1. NC_000913.3.
    WP_000132231.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC75742; AAC75742; b2700.
    BAA16562; BAA16562; BAA16562.
    GeneIDi947169.
    KEGGiecj:JW2670.
    eco:b2700.
    PATRICi32120798. VBIEscCol129921_2791.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U18785 Genomic DNA. Translation: AAB60061.1.
    U00096 Genomic DNA. Translation: AAC75742.1.
    AP009048 Genomic DNA. Translation: BAA16562.1.
    V00328 Genomic DNA. No translation available.
    PIRiH65049.
    RefSeqiNP_417180.1. NC_000913.3.
    WP_000132231.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP0A6G3.
    SMRiP0A6G3. Positions 7-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4262070. 139 interactions.
    DIPiDIP-48189N.
    IntActiP0A6G3. 8 interactions.
    STRINGi511145.b2700.

    Proteomic databases

    EPDiP0A6G3.
    PaxDbiP0A6G3.
    PRIDEiP0A6G3.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC75742; AAC75742; b2700.
    BAA16562; BAA16562; BAA16562.
    GeneIDi947169.
    KEGGiecj:JW2670.
    eco:b2700.
    PATRICi32120798. VBIEscCol129921_2791.

    Organism-specific databases

    EchoBASEiEB2560.
    EcoGeneiEG12698. pncC.

    Phylogenomic databases

    eggNOGiENOG4107TH8. Bacteria.
    COG1546. LUCA.
    HOGENOMiHOG000242527.
    InParanoidiP0A6G3.
    KOiK03743.
    OMAiTVWFAWA.
    OrthoDBiEOG61S359.
    PhylomeDBiP0A6G3.

    Enzyme and pathway databases

    BioCyciEcoCyc:G7409-MONOMER.
    ECOL316407:JW2670-MONOMER.
    MetaCyc:G7409-MONOMER.

    Miscellaneous databases

    PROiP0A6G3.

    Family and domain databases

    Gene3Di3.90.950.20. 1 hit.
    InterProiIPR008136. CinA_C.
    [Graphical view]
    PfamiPF02464. CinA. 1 hit.
    [Graphical view]
    SUPFAMiSSF142433. SSF142433. 1 hit.
    TIGRFAMsiTIGR00199. PncC_domain. 1 hit.
    ProtoNetiSearch...

    Publicationsi

    « Hide 'large scale' publications
    1. "Cloning and expression of a murein hydrolase lipoprotein from Escherichia coli."
      Ehlert K., Hoeltje J.-V., Templin M.F.
      Mol. Microbiol. 16:761-768(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: K12.
    2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
      Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
      , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
      DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-165.
    6. "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography."
      Fountoulakis M., Takacs M.-F., Berndt P., Langen H., Takacs B.
      Electrophoresis 20:2181-2195(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: B / BL21.
    7. "Identification of nicotinamide mononucleotide deamidase of the bacterial pyridine nucleotide cycle reveals a novel broadly conserved amidohydrolase family."
      Galeazzi L., Bocci P., Amici A., Brunetti L., Ruggieri S., Romine M., Reed S., Osterman A.L., Rodionov D.A., Sorci L., Raffaelli N.
      J. Biol. Chem. 286:40365-40375(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
      Strain: K12.

    Entry informationi

    Entry nameiPNCC_ECOLI
    AccessioniPrimary (citable) accession number: P0A6G3
    Secondary accession number(s): P41053
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 29, 2005
    Last sequence update: March 29, 2005
    Last modified: March 16, 2016
    This is version 86 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.