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P0A6F2

- CARA_ECO57

UniProt

P0A6F2 - CARA_ECO57

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Protein

Carbamoyl-phosphate synthase small chain

Gene
carA, pyrA, Z0037, ECs0035
Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei269 – 2691Nucleophile By similarity
Active sitei353 – 3531 By similarity
Active sitei355 – 3551 By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
  2. arginine biosynthetic process Source: UniProtKB-HAMAP
  3. carbamoyl phosphate biosynthetic process Source: InterPro
  4. glutamine catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-33-MONOMER.
ECOO157:CARA-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:carA
Synonyms:pyrA
Ordered Locus Names:Z0037, ECs0035
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Carbamoyl-phosphate synthase small chainUniRule annotationPRO_0000112275Add
BLAST

Proteomic databases

PRIDEiP0A6F2.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

MINTiMINT-1255849.
STRINGi155864.Z0037.

Structurei

3D structure databases

ProteinModelPortaliP0A6F2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini193 – 380188Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 189189CPSaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the CarA family.

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0505.
HOGENOMiHOG000038087.
KOiK01956.
OMAiKTIKMKF.
OrthoDBiEOG61ZTH6.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6F2-1 [UniParc]FASTAAdd to Basket

« Hide

MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR    50
QIVTLTYPHI GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS 100
YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA ALALEKARAF 150
PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG 200
AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY 250
AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV 300
EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF 350
QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK 382
Length:382
Mass (Da):41,431
Last modified:July 21, 1986 - v1
Checksum:i60BC26366417443F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG54334.1.
BA000007 Genomic DNA. Translation: BAB33458.1.
PIRiC90633.
RefSeqiNP_285726.1. NC_002655.2.
NP_308062.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG54334; AAG54334; Z0037.
BAB33458; BAB33458; BAB33458.
GeneIDi913431.
956698.
KEGGiece:Z0037.
ecs:ECs0035.
PATRICi18349004. VBIEscCol44059_0032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE005174 Genomic DNA. Translation: AAG54334.1 .
BA000007 Genomic DNA. Translation: BAB33458.1 .
PIRi C90633.
RefSeqi NP_285726.1. NC_002655.2.
NP_308062.1. NC_002695.1.

3D structure databases

ProteinModelPortali P0A6F2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-1255849.
STRINGi 155864.Z0037.

Proteomic databases

PRIDEi P0A6F2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAG54334 ; AAG54334 ; Z0037 .
BAB33458 ; BAB33458 ; BAB33458 .
GeneIDi 913431.
956698.
KEGGi ece:Z0037.
ecs:ECs0035.
PATRICi 18349004. VBIEscCol44059_0032.

Phylogenomic databases

eggNOGi COG0505.
HOGENOMi HOG000038087.
KOi K01956.
OMAi KTIKMKF.
OrthoDBi EOG61ZTH6.

Enzyme and pathway databases

UniPathwayi UPA00068 ; UER00171 .
UPA00070 ; UER00115 .
BioCyci ECOL386585:GJFA-33-MONOMER.
ECOO157:CARA-MONOMER.

Family and domain databases

Gene3Di 3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPi MF_01209. CPSase_S_chain.
InterProi IPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view ]
Pfami PF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view ]
SMARTi SM01097. CPSase_sm_chain. 1 hit.
[Graphical view ]
SUPFAMi SSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR01368. CPSaseIIsmall. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiCARA_ECO57
AccessioniPrimary (citable) accession number: P0A6F2
Secondary accession number(s): P00907
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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