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P0A6F2 (CARA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbamoyl-phosphate synthase small chain

EC=6.3.5.5
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene names
Name:carA
Synonyms:pyrA
Ordered Locus Names:Z0037, ECs0035
OrganismEscherichia coli O157:H7 [Complete proteome] [HAMAP]
Taxonomic identifier83334 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP-Rule MF_01209

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP-Rule MF_01209

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP-Rule MF_01209

Subunit structure

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Sequence similarities

Belongs to the CarA family.

Contains 1 glutamine amidotransferase type-1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Carbamoyl-phosphate synthase small chain HAMAP-Rule MF_01209
PRO_0000112275

Regions

Domain193 – 380188Glutamine amidotransferase type-1
Region1 – 189189CPSase HAMAP-Rule MF_01209

Sites

Active site2691Nucleophile By similarity
Active site3531 By similarity
Active site3551 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A6F2 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 60BC26366417443F

FASTA38241,431
        10         20         30         40         50         60 
MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR QIVTLTYPHI 

        70         80         90        100        110        120 
GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS YLKRHNIVAI ADIDTRKLTR 

       130        140        150        160        170        180 
LLREKGAQNG CIIAGDNPDA ALALEKARAF PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG 

       190        200        210        220        230        240 
LPEAKKEDEL PFHVVAYDFG AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN 

       250        260        270        280        290        300 
GPGDPAPCDY AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV 

       310        320        330        340        350        360 
EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF QGHPEASPGP 

       370        380 
HDAAPLFDHF IELIEQYRKT AK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE005174 Genomic DNA. Translation: AAG54334.1.
BA000007 Genomic DNA. Translation: BAB33458.1.
PIRC90633.
RefSeqNP_285726.1. NC_002655.2.
NP_308062.1. NC_002695.1.

3D structure databases

ProteinModelPortalP0A6F2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-1255849.
STRING155864.Z0037.

Proteomic databases

PRIDEP0A6F2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAG54334; AAG54334; Z0037.
BAB33458; BAB33458; BAB33458.
GeneID913431.
956698.
KEGGece:Z0037.
ecs:ECs0035.
PATRIC18349004. VBIEscCol44059_0032.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0505.
HOGENOMHOG000038087.
KOK01956.
OMAKTIKMKF.
OrthoDBEOG61ZTH6.

Enzyme and pathway databases

BioCycECOL386585:GJFA-33-MONOMER.
ECOO157:CARA-MONOMER.
UniPathwayUPA00068; UER00171.
UPA00070; UER00115.

Family and domain databases

Gene3D3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPMF_01209. CPSase_S_chain.
InterProIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCARA_ECO57
AccessionPrimary (citable) accession number: P0A6F2
Secondary accession number(s): P00907
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways