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P0A6F2

- CARA_ECO57

UniProt

P0A6F2 - CARA_ECO57

Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Escherichia coli O157:H7
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (21 Jul 1986)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei269 – 2691NucleophileBy similarity
    Active sitei353 – 3531By similarity
    Active sitei355 – 3551By similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    2. arginine biosynthetic process Source: UniProtKB-HAMAP
    3. carbamoyl phosphate biosynthetic process Source: InterPro
    4. glutamine catabolic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciECOL386585:GJFA-33-MONOMER.
    ECOO157:CARA-MONOMER.
    UniPathwayiUPA00068; UER00171.
    UPA00070; UER00115.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbamoyl-phosphate synthase small chain (EC:6.3.5.5)
    Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain
    Gene namesi
    Name:carA
    Synonyms:pyrA
    Ordered Locus Names:Z0037, ECs0035
    OrganismiEscherichia coli O157:H7
    Taxonomic identifieri83334 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000558: Chromosome, UP000002519: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 382382Carbamoyl-phosphate synthase small chainPRO_0000112275Add
    BLAST

    Proteomic databases

    PRIDEiP0A6F2.

    Interactioni

    Subunit structurei

    Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

    Protein-protein interaction databases

    MINTiMINT-1255849.
    STRINGi155864.Z0037.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6F2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini193 – 380188Glutamine amidotransferase type-1Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 189189CPSaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the CarA family.Curated

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    eggNOGiCOG0505.
    HOGENOMiHOG000038087.
    KOiK01956.
    OMAiKTIKMKF.
    OrthoDBiEOG61ZTH6.

    Family and domain databases

    Gene3Di3.40.50.880. 1 hit.
    3.50.30.20. 1 hit.
    HAMAPiMF_01209. CPSase_S_chain.
    InterProiIPR006274. CarbamoylP_synth_ssu.
    IPR002474. CarbamoylP_synth_ssu_N.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    [Graphical view]
    PfamiPF00988. CPSase_sm_chain. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view]
    SMARTiSM01097. CPSase_sm_chain. 1 hit.
    [Graphical view]
    SUPFAMiSSF52021. SSF52021. 1 hit.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A6F2-1 [UniParc]FASTAAdd to Basket

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    MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR    50
    QIVTLTYPHI GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS 100
    YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA ALALEKARAF 150
    PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG 200
    AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY 250
    AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV 300
    EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF 350
    QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK 382
    Length:382
    Mass (Da):41,431
    Last modified:July 21, 1986 - v1
    Checksum:i60BC26366417443F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG54334.1.
    BA000007 Genomic DNA. Translation: BAB33458.1.
    PIRiC90633.
    RefSeqiNP_285726.1. NC_002655.2.
    NP_308062.1. NC_002695.1.

    Genome annotation databases

    EnsemblBacteriaiAAG54334; AAG54334; Z0037.
    BAB33458; BAB33458; BAB33458.
    GeneIDi913431.
    956698.
    KEGGiece:Z0037.
    ecs:ECs0035.
    PATRICi18349004. VBIEscCol44059_0032.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE005174 Genomic DNA. Translation: AAG54334.1 .
    BA000007 Genomic DNA. Translation: BAB33458.1 .
    PIRi C90633.
    RefSeqi NP_285726.1. NC_002655.2.
    NP_308062.1. NC_002695.1.

    3D structure databases

    ProteinModelPortali P0A6F2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-1255849.
    STRINGi 155864.Z0037.

    Proteomic databases

    PRIDEi P0A6F2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAG54334 ; AAG54334 ; Z0037 .
    BAB33458 ; BAB33458 ; BAB33458 .
    GeneIDi 913431.
    956698.
    KEGGi ece:Z0037.
    ecs:ECs0035.
    PATRICi 18349004. VBIEscCol44059_0032.

    Phylogenomic databases

    eggNOGi COG0505.
    HOGENOMi HOG000038087.
    KOi K01956.
    OMAi KTIKMKF.
    OrthoDBi EOG61ZTH6.

    Enzyme and pathway databases

    UniPathwayi UPA00068 ; UER00171 .
    UPA00070 ; UER00115 .
    BioCyci ECOL386585:GJFA-33-MONOMER.
    ECOO157:CARA-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.880. 1 hit.
    3.50.30.20. 1 hit.
    HAMAPi MF_01209. CPSase_S_chain.
    InterProi IPR006274. CarbamoylP_synth_ssu.
    IPR002474. CarbamoylP_synth_ssu_N.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    [Graphical view ]
    Pfami PF00988. CPSase_sm_chain. 1 hit.
    PF00117. GATase. 1 hit.
    [Graphical view ]
    SMARTi SM01097. CPSase_sm_chain. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52021. SSF52021. 1 hit.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR01368. CPSaseIIsmall. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
    2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
      Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
      , Kuhara S., Shiba T., Hattori M., Shinagawa H.
      DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

    Entry informationi

    Entry nameiCARA_ECO57
    AccessioniPrimary (citable) accession number: P0A6F2
    Secondary accession number(s): P00907
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3