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Protein

Carbamoyl-phosphate synthase small chain

Gene

carA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei269 – 2691NucleophileBy similarity
Active sitei353 – 3531By similarity
Active sitei355 – 3551By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciECOL386585:GJFA-33-MONOMER.
ECOO157:CARA-MONOMER.
UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbamoyl-phosphate synthase small chain (EC:6.3.5.5)
Alternative name(s):
Carbamoyl-phosphate synthetase glutamine chain
Gene namesi
Name:carA
Synonyms:pyrA
Ordered Locus Names:Z0037, ECs0035
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000558 Componenti: Chromosome UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Carbamoyl-phosphate synthase small chainPRO_0000112275Add
BLAST

Proteomic databases

PRIDEiP0A6F2.

Interactioni

Subunit structurei

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

MINTiMINT-1255849.
STRINGi155864.Z0037.

Structurei

3D structure databases

ProteinModelPortaliP0A6F2.
SMRiP0A6F2. Positions 2-380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini193 – 380188Glutamine amidotransferase type-1Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 189189CPSaseAdd
BLAST

Sequence similaritiesi

Belongs to the CarA family.Curated

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiCOG0505.
HOGENOMiHOG000038087.
KOiK01956.
OMAiPHDSHYL.
OrthoDBiEOG61ZTH6.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6F2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR
60 70 80 90 100
QIVTLTYPHI GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS
110 120 130 140 150
YLKRHNIVAI ADIDTRKLTR LLREKGAQNG CIIAGDNPDA ALALEKARAF
160 170 180 190 200
PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG LPEAKKEDEL PFHVVAYDFG
210 220 230 240 250
AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN GPGDPAPCDY
260 270 280 290 300
AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV
310 320 330 340 350
EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF
360 370 380
QGHPEASPGP HDAAPLFDHF IELIEQYRKT AK
Length:382
Mass (Da):41,431
Last modified:July 21, 1986 - v1
Checksum:i60BC26366417443F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54334.1.
BA000007 Genomic DNA. Translation: BAB33458.1.
PIRiC90633.
RefSeqiNP_308062.1. NC_002695.1.

Genome annotation databases

EnsemblBacteriaiAAG54334; AAG54334; Z0037.
BAB33458; BAB33458; BAB33458.
GeneIDi913431.
KEGGiece:Z0037.
ecs:ECs0035.
PATRICi18349004. VBIEscCol44059_0032.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG54334.1.
BA000007 Genomic DNA. Translation: BAB33458.1.
PIRiC90633.
RefSeqiNP_308062.1. NC_002695.1.

3D structure databases

ProteinModelPortaliP0A6F2.
SMRiP0A6F2. Positions 2-380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1255849.
STRINGi155864.Z0037.

Chemistry

BindingDBiP0A6F2.

Proteomic databases

PRIDEiP0A6F2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG54334; AAG54334; Z0037.
BAB33458; BAB33458; BAB33458.
GeneIDi913431.
KEGGiece:Z0037.
ecs:ECs0035.
PATRICi18349004. VBIEscCol44059_0032.

Phylogenomic databases

eggNOGiCOG0505.
HOGENOMiHOG000038087.
KOiK01956.
OMAiPHDSHYL.
OrthoDBiEOG61ZTH6.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00171.
UPA00070; UER00115.
BioCyciECOL386585:GJFA-33-MONOMER.
ECOO157:CARA-MONOMER.

Family and domain databases

Gene3Di3.40.50.880. 1 hit.
3.50.30.20. 1 hit.
HAMAPiMF_01209. CPSase_S_chain.
InterProiIPR006274. CarbamoylP_synth_ssu.
IPR002474. CarbamoylP_synth_ssu_N.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
[Graphical view]
PfamiPF00988. CPSase_sm_chain. 1 hit.
PF00117. GATase. 1 hit.
[Graphical view]
SMARTiSM01097. CPSase_sm_chain. 1 hit.
[Graphical view]
SUPFAMiSSF52021. SSF52021. 1 hit.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR01368. CPSaseIIsmall. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.
  2. "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12."
    Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T.
    , Kuhara S., Shiba T., Hattori M., Shinagawa H.
    DNA Res. 8:11-22(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Entry informationi

Entry nameiCARA_ECO57
AccessioniPrimary (citable) accession number: P0A6F2
Secondary accession number(s): P00907
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: May 27, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.