Reviewed, UniProtKB/Swiss-Prot P0A6E9 (BIOD2_ECOLI)

Names and origin

Protein names

Recommended name:
    Putative dethiobiotin synthetase
      Short name=Dethiobiotin synthase
      Short name=DTB synthetase
      Short name=DTBS
    EC=6.3.3.3

Gene names

Name:	ynfK
Ordered Locus Names:	b1593, JW5264

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: HAMAP cobalamin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cobyrinic acid a,c-diamide synthase activity Inferred from electronic annotation. Source: InterPro dethiobiotin synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Putative dethiobiotin synthetase HAMAP MF_00336		PRO_0000187962
Regions
	Nucleotide binding	9 – 17	9	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A6E9-1 [UniParc]. Last modified June 7, 2005. Version 1. Checksum: 35A04911309A99B0	FASTA	231	24,981
	10 20 30 40 50 60 MLKRFFITGT DTSVGKTVVS RALLQALASQ GKTVAGYKPV AKGSKETPEG LRNKDALVLQ 70 80 90 100 110 120 SVSTIELPYE AVNPIALSEE ESSVAHSCPI NYTLISNGLA NLTEKVDHVV VEGTGGWRSL 130 140 150 160 170 180 MNDLRPLSEW VVQEQLPVLM VVGIQEGCIN HALLTAQAIA NDGLPLIGWV ANRINPGLAH 190 200 210 220 230 YAEIIDVLGK KLPAPLIGEL PYLPRAEQRE LGQYIRLAML RSVLAVDRVT V

References

[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:363-377(1996) [PubMed: 9097039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U00096 Genomic DNA. Translation: AAC74665.1. Different initiation. AP009048 Genomic DNA. Translation: BAA15317.1. Different initiation.
PIR	C64915.
RefSeq	AP_002214.1. NP_416110.4.
3D structure databases
HSSP	HSSP built from PDB template 1DAI based on UniProtKB P13000.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:28066N.
STRING	P0A6E9.
Genome annotation databases
GeneID	944927.
GenomeReviews	Gene locus JW5264 in contig AP009048_GR. Gene locus b1593 in contig U00096_GR.
KEGG	ecj:JW5264. eco:b1593.
Organism-specific databases
EchoBASE	EB3610.
EcoGene	EG13849. ynfK.
CMR	Search...
Phylogenomic databases
HOGENOM	P0A6E9.
OMA	ETPEGMR.
Enzyme and pathway databases
BioCyc	EcoCyc:G6851-MON.
Gene expression databases
Genevestigator	P0A6E9.
Family and domain databases
HAMAP	MF_00336. [Tree]
InterPro	IPR004472. BioD_synth. IPR002586. CbiA_P_synth. [Graphical view]
Pfam	PF01656. CbiA. 1 hit. [Graphical view]
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. bioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD2_ECOLI

Accession

Primary (citable) accession number: P0A6E9
Secondary accession number(s): P77201, Q8X788

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)