Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Shikimate kinase 2

Gene

aroL

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.2 Publications

Miscellaneous

Two isozymes have been found in E.coli. AroK has 100-fold lower affinity for shikimate than AroL, suggesting that AroL is the dominant enzyme in the biosynthesis of the aromatic amino acids, with AroK playing a secondary role and possibly participating in an as yet unidentified cellular process.

Catalytic activityi

ATP + shikimate = ADP + shikimate 3-phosphate.

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Kineticsi

  1. KM=200 µM for shikimate1 Publication
  2. KM=160 µM for ATP1 Publication

    Pathwayi: chorismate biosynthesis

    This protein is involved in step 5 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.
    Proteins known to be involved in the 7 steps of the subpathway in this organism are:
    1. Phospho-2-dehydro-3-deoxyheptonate aldolase, Tyr-sensitive (aroF), Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH), Phospho-2-dehydro-3-deoxyheptonate aldolase, Phe-sensitive (aroG)
    2. 3-dehydroquinate synthase (aroB)
    3. 3-dehydroquinate dehydratase (aroD)
    4. Quinate/shikimate dehydrogenase (ydiB), Shikimate dehydrogenase (NADP(+)) (aroE)
    5. Shikimate kinase 2 (aroL), Shikimate kinase 1 (aroK)
    6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
    7. Chorismate synthase (aroC)
    This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi16MagnesiumBy similarity1
    Metal bindingi32MagnesiumBy similarity1
    Binding sitei34SubstrateBy similarity1
    Binding sitei58SubstrateBy similarity1
    Binding sitei79Substrate; via amide nitrogenBy similarity1
    Binding sitei120ATPBy similarity1
    Binding sitei139SubstrateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi12 – 17ATPBy similarity6

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • magnesium ion binding Source: EcoCyc
    • metal ion binding Source: EcoCyc
    • shikimate kinase activity Source: EcoCyc

    GO - Biological processi

    • aromatic amino acid family biosynthetic process Source: EcoliWiki
    • chorismate biosynthetic process Source: UniProtKB-UniPathway
    • shikimate metabolic process Source: GO_Central

    Keywordsi

    Molecular functionKinase, Transferase
    Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:AROL-MONOMER
    MetaCyc:AROL-MONOMER
    SABIO-RKiP0A6E1
    UniPathwayiUPA00053; UER00088

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shikimate kinase 2 (EC:2.7.1.71)
    Short name:
    SK 2
    Alternative name(s):
    Shikimate kinase II
    Short name:
    SKII
    Gene namesi
    Name:aroL
    Ordered Locus Names:b0388, JW0379
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG10082 aroL

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemoved2 Publications
    ChainiPRO_00001923782 – 174Shikimate kinase 2Add BLAST173

    Proteomic databases

    PaxDbiP0A6E1
    PRIDEiP0A6E1

    Expressioni

    Inductioni

    Expressed under the control of TyrR and TrpR repressors.3 Publications

    Interactioni

    Subunit structurei

    Monomer.1 Publication

    Protein-protein interaction databases

    BioGridi4259831, 6 interactors
    IntActiP0A6E1, 3 interactors
    STRINGi316385.ECDH10B_0345

    Structurei

    3D structure databases

    ProteinModelPortaliP0A6E1
    SMRiP0A6E1
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni112 – 126LID domainAdd BLAST15

    Domaini

    The LID domain closes over the active site upon ATP binding.By similarity

    Sequence similaritiesi

    Belongs to the shikimate kinase family. AroL subfamily.Curated

    Phylogenomic databases

    eggNOGiENOG4105KHV Bacteria
    COG0703 LUCA
    HOGENOMiHOG000032568
    InParanoidiP0A6E1
    KOiK00891
    OMAiKHLFERS
    PhylomeDBiP0A6E1

    Family and domain databases

    CDDicd00464 SK, 1 hit
    HAMAPiMF_00109 Shikimate_kinase, 1 hit
    MF_01269 Shikimate_kinase_2, 1 hit
    InterProiView protein in InterPro
    IPR027417 P-loop_NTPase
    IPR031322 Shikimate/glucono_kinase
    IPR000623 Shikimate_kinase/TSH1
    IPR027544 Shikimate_kinase_2
    IPR023000 Shikimate_kinase_CS
    PfamiView protein in Pfam
    PF01202 SKI, 1 hit
    SUPFAMiSSF52540 SSF52540, 1 hit
    PROSITEiView protein in PROSITE
    PS01128 SHIKIMATE_KINASE, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A6E1-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MTQPLFLIGP RGCGKTTVGM ALADSLNRRF VDTDQWLQSQ LNMTVAEIVE
    60 70 80 90 100
    REEWAGFRAR ETAALEAVTA PSTVIATGGG IILTEFNRHF MQNNGIVVYL
    110 120 130 140 150
    CAPVSVLVNR LQAAPEEDLR PTLTGKPLSE EVQEVLEERD ALYREVAHII
    160 170
    IDATNEPSQV ISEIRSALAQ TINC
    Length:174
    Mass (Da):19,151
    Last modified:January 23, 2007 - v3
    Checksum:iFCB8D86F6DD55347
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X04064 Genomic DNA Translation: CAA27696.1
    M13045 Genomic DNA Translation: AAA83833.1
    U73857 Genomic DNA Translation: AAB18112.1
    U00096 Genomic DNA Translation: AAC73491.1
    AP009048 Genomic DNA Translation: BAE76169.1
    PIRiA90333 KIECS
    RefSeqiNP_414922.1, NC_000913.3
    WP_000193393.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC73491; AAC73491; b0388
    BAE76169; BAE76169; BAE76169
    GeneIDi945031
    KEGGiecj:JW0379
    eco:b0388
    PATRICifig|1411691.4.peg.1890

    Similar proteinsi

    Entry informationi

    Entry nameiAROL_ECOLI
    AccessioniPrimary (citable) accession number: P0A6E1
    Secondary accession number(s): P08329, Q2MC37
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: January 23, 2007
    Last modified: March 28, 2018
    This is version 98 of the entry and version 3 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health