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P0A6D3

- AROA_ECOLI

UniProt

P0A6D3 - AROA_ECOLI

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Protein
3-phosphoshikimate 1-carboxyvinyltransferase
Gene
aroA, b0908, JW0891
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei408 – 4081Modified by bromopyruvate
Sitei411 – 4111Modified by bromopyruvate

GO - Molecular functioni

  1. 3-phosphoshikimate 1-carboxyvinyltransferase activity Source: EcoCyc

GO - Biological processi

  1. aromatic amino acid family biosynthetic process Source: UniProtKB-KW
  2. chorismate biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:AROA-MONOMER.
ECOL316407:JW0891-MONOMER.
MetaCyc:AROA-MONOMER.
SABIO-RKP0A6D3.
UniPathwayiUPA00053; UER00089.

Names & Taxonomyi

Protein namesi
Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase (EC:2.5.1.19)
Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name:
EPSP synthase
Short name:
EPSPS
Gene namesi
Name:aroA
Ordered Locus Names:b0908, JW0891
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG10073. aroA.

Subcellular locationi

Cytoplasm Inferred UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4274273-phosphoshikimate 1-carboxyvinyltransferaseUniRule annotation
PRO_0000088253Add
BLAST

Proteomic databases

PaxDbiP0A6D3.
PRIDEiP0A6D3.

2D gel databases

SWISS-2DPAGEP0A6D3.

Expressioni

Gene expression databases

GenevestigatoriP0A6D3.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

DIPiDIP-48256N.
IntActiP0A6D3. 3 interactions.
STRINGi511145.b0908.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 64
Beta strandi11 – 177
Helixi22 – 3413
Beta strandi35 – 439
Helixi48 – 5912
Beta strandi63 – 664
Beta strandi68 – 703
Beta strandi73 – 764
Beta strandi79 – 813
Beta strandi88 – 914
Helixi96 – 10510
Beta strandi108 – 1169
Helixi119 – 1235
Helixi127 – 1359
Beta strandi140 – 1456
Beta strandi151 – 1555
Beta strandi160 – 1689
Helixi171 – 1799
Helixi180 – 1823
Beta strandi183 – 1853
Beta strandi187 – 1959
Helixi199 – 21012
Beta strandi216 – 2183
Turni219 – 2213
Beta strandi222 – 2254
Beta strandi235 – 2384
Helixi243 – 25614
Beta strandi257 – 2648
Helixi272 – 2754
Helixi276 – 2838
Beta strandi286 – 2894
Beta strandi291 – 2977
Beta strandi305 – 3073
Turni312 – 3143
Helixi315 – 3217
Helixi322 – 3243
Beta strandi325 – 3273
Beta strandi329 – 3335
Helixi335 – 3395
Beta strandi340 – 3423
Helixi344 – 35411
Beta strandi358 – 3614
Beta strandi363 – 3697
Helixi385 – 3928
Helixi393 – 3953
Beta strandi396 – 3994
Beta strandi401 – 4055
Helixi406 – 4116
Helixi416 – 4238

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EPSX-ray3.00A1-427[»]
1G6SX-ray1.50A1-427[»]
1G6TX-ray1.60A1-427[»]
1MI4X-ray1.70A1-427[»]
1P88NMR-A25-240[»]
1P89NMR-A25-240[»]
1Q0Imodel-A1-427[»]
1Q0Jmodel-A1-427[»]
1Q36X-ray1.60A1-427[»]
1X8RX-ray1.50A1-427[»]
1X8TX-ray1.90A1-427[»]
2AA9X-ray1.50A1-427[»]
2AAYX-ray1.55A1-427[»]
2PQ9X-ray1.60A1-427[»]
2QFQX-ray1.50A1-427[»]
2QFSX-ray1.55A1-427[»]
2QFTX-ray1.55A1-427[»]
2QFUX-ray1.60A1-427[»]
3FJXX-ray1.75A1-427[»]
3FJZX-ray1.70A1-427[»]
3FK0X-ray1.70A1-427[»]
3FK1X-ray1.70A1-427[»]
ProteinModelPortaliP0A6D3.
SMRiP0A6D3. Positions 1-427.

Miscellaneous databases

EvolutionaryTraceiP0A6D3.

Family & Domainsi

Sequence similaritiesi

Belongs to the EPSP synthase family.

Phylogenomic databases

eggNOGiCOG0128.
HOGENOMiHOG000247372.
KOiK00800.
OMAiGADIEWG.
OrthoDBiEOG6Z6FZ4.
PhylomeDBiP0A6D3.

Family and domain databases

Gene3Di3.65.10.10. 2 hits.
HAMAPiMF_00210. EPSP_synth.
InterProiIPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view]
PfamiPF00275. EPSP_synthase. 1 hit.
[Graphical view]
PIRSFiPIRSF000505. EPSPS. 1 hit.
SUPFAMiSSF55205. SSF55205. 1 hit.
TIGRFAMsiTIGR01356. aroA. 1 hit.
PROSITEiPS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6D3-1 [UniParc]FASTAAdd to Basket

« Hide

MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV    50
RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR 100
PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP 150
LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY 200
IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE GDASSASYFL 250
AAAAIKGGTV KVTGIGRNSM QGDIRFADVL EKMGATICWG DDYISCTRGE 300
LNAIDMDMNH IPDAAMTIAT AALFAKGTTT LRNIYNWRVK ETDRLFAMAT 350
ELRKVGAEVE EGHDYIRITP PEKLNFAEIA TYNDHRMAMC FSLVALSDTP 400
VTILDPKCTA KTFPDYFEQL ARISQAA 427
Length:427
Mass (Da):46,096
Last modified:June 7, 2005 - v1
Checksum:iB1F55469EB4D9F97
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti96 – 961G → A Confers glyphosate inhibition.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti23 – 231S → T in CAA25223. 1 Publication
Sequence conflicti330 – 3301T → R in CAA25223. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00557 Genomic DNA. Translation: CAA25223.1.
U00096 Genomic DNA. Translation: AAC73994.1.
AP009048 Genomic DNA. Translation: BAA35643.1.
U31523 Genomic DNA. Translation: AAA81514.1.
PIRiC64830. XUECVS.
RefSeqiNP_415428.1. NC_000913.3.
YP_489180.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC73994; AAC73994; b0908.
BAA35643; BAA35643; BAA35643.
GeneIDi12931017.
945528.
KEGGiecj:Y75_p0880.
eco:b0908.
PATRICi32117029. VBIEscCol129921_0939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X00557 Genomic DNA. Translation: CAA25223.1 .
U00096 Genomic DNA. Translation: AAC73994.1 .
AP009048 Genomic DNA. Translation: BAA35643.1 .
U31523 Genomic DNA. Translation: AAA81514.1 .
PIRi C64830. XUECVS.
RefSeqi NP_415428.1. NC_000913.3.
YP_489180.1. NC_007779.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EPS X-ray 3.00 A 1-427 [» ]
1G6S X-ray 1.50 A 1-427 [» ]
1G6T X-ray 1.60 A 1-427 [» ]
1MI4 X-ray 1.70 A 1-427 [» ]
1P88 NMR - A 25-240 [» ]
1P89 NMR - A 25-240 [» ]
1Q0I model - A 1-427 [» ]
1Q0J model - A 1-427 [» ]
1Q36 X-ray 1.60 A 1-427 [» ]
1X8R X-ray 1.50 A 1-427 [» ]
1X8T X-ray 1.90 A 1-427 [» ]
2AA9 X-ray 1.50 A 1-427 [» ]
2AAY X-ray 1.55 A 1-427 [» ]
2PQ9 X-ray 1.60 A 1-427 [» ]
2QFQ X-ray 1.50 A 1-427 [» ]
2QFS X-ray 1.55 A 1-427 [» ]
2QFT X-ray 1.55 A 1-427 [» ]
2QFU X-ray 1.60 A 1-427 [» ]
3FJX X-ray 1.75 A 1-427 [» ]
3FJZ X-ray 1.70 A 1-427 [» ]
3FK0 X-ray 1.70 A 1-427 [» ]
3FK1 X-ray 1.70 A 1-427 [» ]
ProteinModelPortali P0A6D3.
SMRi P0A6D3. Positions 1-427.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48256N.
IntActi P0A6D3. 3 interactions.
STRINGi 511145.b0908.

Chemistry

BindingDBi P0A6D3.
ChEMBLi CHEMBL5033.

2D gel databases

SWISS-2DPAGE P0A6D3.

Proteomic databases

PaxDbi P0A6D3.
PRIDEi P0A6D3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC73994 ; AAC73994 ; b0908 .
BAA35643 ; BAA35643 ; BAA35643 .
GeneIDi 12931017.
945528.
KEGGi ecj:Y75_p0880.
eco:b0908.
PATRICi 32117029. VBIEscCol129921_0939.

Organism-specific databases

EchoBASEi EB0071.
EcoGenei EG10073. aroA.

Phylogenomic databases

eggNOGi COG0128.
HOGENOMi HOG000247372.
KOi K00800.
OMAi GADIEWG.
OrthoDBi EOG6Z6FZ4.
PhylomeDBi P0A6D3.

Enzyme and pathway databases

UniPathwayi UPA00053 ; UER00089 .
BioCyci EcoCyc:AROA-MONOMER.
ECOL316407:JW0891-MONOMER.
MetaCyc:AROA-MONOMER.
SABIO-RK P0A6D3.

Miscellaneous databases

EvolutionaryTracei P0A6D3.
PROi P0A6D3.

Gene expression databases

Genevestigatori P0A6D3.

Family and domain databases

Gene3Di 3.65.10.10. 2 hits.
HAMAPi MF_00210. EPSP_synth.
InterProi IPR001986. Enolpyruvate_Tfrase_dom.
IPR006264. EPSP_synthase.
IPR023193. EPSP_synthase_CS.
IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b.
[Graphical view ]
Pfami PF00275. EPSP_synthase. 1 hit.
[Graphical view ]
PIRSFi PIRSF000505. EPSPS. 1 hit.
SUPFAMi SSF55205. SSF55205. 1 hit.
TIGRFAMsi TIGR01356. aroA. 1 hit.
PROSITEi PS00104. EPSP_SYNTHASE_1. 1 hit.
PS00885. EPSP_SYNTHASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The complete amino acid sequence of Escherichia coli 5-enolpyruvylshikimate 3-phosphate synthase."
    Duncan K., Lewendon A., Coggins J.R.
    FEBS Lett. 170:59-63(1984)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 380-427.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli --the substrate analogue bromopyruvate inactivates the enzyme by modifying Cys-408 and Lys-411."
    Huynh Q.K.
    Arch. Biochem. Biophys. 284:407-412(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: INHIBITION BY BROMOPYRUVATE.
  7. "Photo-oxidation of 5-enolpyruvoylshikimate-3-phosphate synthase from Escherichia coli: evidence for a reactive imidazole group (His385) at the herbicide glyphosate-binding site."
    Huynh Q.K.
    Biochem. J. 290:525-530(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOTO-OXIDATION AT HIS-385.
  8. "Structure and topological symmetry of the glyphosate target 5-enol-pyruvylshikimate-3-phosphate synthase: a distinctive protein fold."
    Stallings W.C., Abdel-Meguid S.S., Lim L.W., Shieh H.-S., Dayringer H.E., Leimgruber N.K., Stegeman R.A., Anderson K.S., Sikorski J.A., Padgette S.R., Kishore G.M.
    Proc. Natl. Acad. Sci. U.S.A. 88:5046-5050(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Entry informationi

Entry nameiAROA_ECOLI
AccessioniPrimary (citable) accession number: P0A6D3
Secondary accession number(s): P07638, P78222
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: June 7, 2005
Last modified: May 14, 2014
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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