Arginine repressor - Escherichia coli (strain K12)

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P0A6D0 (ARGR_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 63. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Arginine repressor

Gene names

Name:	argR
Synonyms:	xerA
Ordered Locus Names:	b3237, JW3206

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	156 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Negatively controls the expression of the four operons of arginine biosynthesis in addition to the carAB operon. Predominantly interacts with A/T residues in ARG boxes. It also binds to a specific site in cer locus. Thus it is essential for cer-mediated site-specific recombination in ColE1. It is necessary for monomerization of the plasmid ColE1. HAMAP MF_00173
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis. [regulation] HAMAP MF_00173
Subunit structure	Homohexamer.
Subcellular location	Cytoplasm HAMAP MF_00173.
Sequence similarities	Belongs to the ArgR family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis DNA recombination Transcription Transcription regulation
Cellular component	Cytoplasm
Ligand	DNA-binding
Molecular function	Repressor
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW plasmid recombination Inferred from mutant phenotype Ref.1. Source: EcoliWiki transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 156

156

Arginine repressor HAMAP MF_00173

PRO_0000205086

Experimental info

Mutagenesis

E → K: Increased affinity for ARG box in the presence of arginine.

Mutagenesis

S → F: Defective binding to ARG box.

Mutagenesis

S → L: Defective binding to ARG box.

Mutagenesis

P → L: Increased affinity for ARG box in the absence of arginine.

Mutagenesis

105

A → V: Defective binding to arginine and to ARG box.

Mutagenesis

123

G → D: Defective binding to arginine and to ARG box. Forms dimers not hexamers.

Secondary structure

156

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A6D0 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 2F104577EFC46537
Show »

FASTA

156

16,995

        10         20         30         40         50         60 
MRSSAKQEEL VKAFKALLKE EKFSSQGEIV AALQEQGFDN INQSKVSRML TKFGAVRTRN 

        70         80         90        100        110        120 
AKMEMVYCLP AELGVPTTSS PLKNLVLDID YNDAVVVIHT SPGAAQLIAR LLDSLGKAEG 

       130        140        150 
ILGTIAGDDT IFTTPANGFT VKDLYEAILE LFDQEL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The arginine repressor is essential for plasmid-stabilizing site-specific recombination at the ColE1 cer locus." Stirling C.J., Szatmari G., Stewart G., Smith M.C.M., Sherratt D.J. EMBO J. 7:4389-4395(1988) [PubMed: 3149585] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Nucleotide sequence of the argR gene of Escherichia coli K-12 and isolation of its product, the arginine repressor." Lim D., Oppenheim J.D., Eckhardt T., Maas W.K. Proc. Natl. Acad. Sci. U.S.A. 84:6697-6701(1987) [PubMed: 3116542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Solution structure of the DNA-binding domain and model for the complex of multifunctional hexameric arginine repressor with DNA." Seelander-Sunnerhagen M., Nilges M., Otting G., Carey J. Nat. Struct. Biol. 4:819-826(1997) [PubMed: 9334747] [Abstract] Cited for: STRUCTURE BY NMR OF 1-78.
[6]	"Structure of the oligomerization and L-arginine binding domain of the arginine repressor of Escherichia coli." van Duyne G.D., Ghosh G., Maas W.K., Sigler P.B. J. Mol. Biol. 256:377-391(1996) [PubMed: 8594204] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 80-156.
[7]	"The DNA-binding domain of the hexameric arginine repressor." Grandori R., Lavoie T.A., Pflumm M., Tian G., Niersbach H., Maas W.K., Fairman R., Carey J. J. Mol. Biol. 254:150-162(1995) [PubMed: 7490739] [Abstract] Cited for: IDENTIFICATION OF DNA-BINDING DOMAIN.
[8]	"Mutant Escherichia coli arginine repressor proteins that fail to bind L-arginine, yet retain the ability to bind their normal DNA-binding sites." Burke M., Merican A.F., Sherratt D.J. Mol. Microbiol. 13:609-618(1994) [PubMed: 7997173] [Abstract] Cited for: MUTAGENESIS.
[9]	"Mutational analysis of the arginine repressor of Escherichia coli." Tian G., Maas W.K. Mol. Microbiol. 13:599-608(1994) [PubMed: 7997172] [Abstract] Cited for: MUTAGENESIS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X13968 Genomic DNA. Translation: CAA32148.1.
M17532 Genomic DNA. Translation: AAA23486.1.
U18997 Genomic DNA. Translation: AAA58039.1.
U00096 Genomic DNA. Translation: AAC76269.1.
AP009048 Genomic DNA. Translation: BAE77280.1.

PIR

A33888.

RefSeq

NP_417704.1. NC_000913.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AOY	NMR	-	A	1-78	[»]
1XXA	X-ray	2.20	A/B/C/D/E/F	80-156	[»]
1XXB	X-ray	2.60	A/B/C/D/E/F	80-156	[»]
1XXC	X-ray	2.80	A/B/C/D/E/F	80-156	[»]

ProteinModelPortal

P0A6D0.

SMR

P0A6D0. Positions 1-78, 81-153.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47999N.

IntAct

P0A6D0. 5 interactions.

MINT

MINT-1252219.

2D gel databases

ECO2DBASE

D014.3. 6TH EDITION.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000005016; EBESCP00000005016; EBESCG00000004094.
EBESCT00000005017; EBESCP00000005017; EBESCG00000004094.
EBESCT00000016350; EBESCP00000015641; EBESCG00000015410.

GeneID

947861.

GenomeReviews

Gene locus JW3206 in contig AP009048_GR.
Gene locus b3237 in contig U00096_GR.

KEGG

ecj:JW3206.
eco:b3237.

PATRIC

32121900. VBIEscCol129921_3334.

Organism-specific databases

EchoBASE

EB0068.

EcoGene

EG10070. argR.

Phylogenomic databases

eggNOG

COG1438.

GeneTree

EBGT00050000010476.

HOGENOM

HBG697909.

OMA

MVYCLPN.

PhylomeDB

P0A6D0.

ProtClustDB

PRK05066.

Enzyme and pathway databases

BioCyc

EcoCyc:PD00194.

Gene expression databases

Genevestigator

P0A6D0.

Family and domain databases

HAMAP

MF_00173. Arg_repressor.
[Tree]

InterPro

IPR001669. Arg_repress.
IPR020899. Arg_repress_C.
IPR020900. Arg_repress_DNA-bd.
IPR011991. WHTH_trsnscrt_rep_DNA-bd.
[Graphical view]

Gene3D

G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.

K03402.

Pfam

PF01316. Arg_repressor. 1 hit.
PF02863. Arg_repressor_C. 1 hit.
[Graphical view]

PRINTS

PR01467. ARGREPRESSOR.

ProDom

PD007402. Arg_repress. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SUPFAM

SSF55252. Arg_repress. 1 hit.

TIGRFAMs

TIGR01529. ArgR_whole. 1 hit.

ProtoNet

Search...

Entry information

Entry name

ARGR_ECOLI

Accession

Primary (citable) accession number: P0A6D0
Secondary accession number(s): P15282, Q2M8X6

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents