P0A6C7 (ARGA_ECO57) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Amino-acid acetyltransferase
EC=2.3.1.1

Alternative name(s):
N-acetylglutamate synthase
Short name=AGS
Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	Z4135, ECs3675

Organism

Escherichia coli O157:H7 [Complete proteome] [HAMAP]

Taxonomic identifier

83334 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subunit structure	Homohexamer Probable.
Subcellular location	Cytoplasm Probable HAMAP MF_01105.
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acetyl-CoA:L-glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186791
Regions
	Domain	296 – 443	148	N-acetyltransferase

Sequences

	Sequence		Length	Mass (Da)
	P0A6C7 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: AE0D84902456797A	FASTA	443	49,195
	10 20 30 40 50 60 MVKERKTELV EGFRHSVPYI NTHRGKTFVI MLGGEAIEHE NFSSIVNDIG LLHSLGIRLV 70 80 90 100 110 120 VVYGARPQID ANLAAHHHEP LYHKNIRVTD AKTLELVKQA AGTLQLDITA RLSMSLNNTP 130 140 150 160 170 180 LQGAHINVVS GNFIIAQPLG VDDGVDYCHS GRIRRIDEDA IHRQLDSGAI VLMGPVAVSV 190 200 210 220 230 240 TGESFNLTSE EIATQLAIKL KAEKMIGFCS SQGVTNDDGD IVSELFPNEA QARVEAQEEK 250 260 270 280 290 300 GDYNSGTVRF LRGAVKACRS GVRRCHLISY QEDGALLQEL FSRDGIGTQI VMESAEQIRR 310 320 330 340 350 360 ATINDIGGIL ELIRPLEQQG ILVRRSREQL EMEIDKFTII QRDNTTIACA ALYPFPEEKI 370 380 390 400 410 420 GEMACVAVHP DYRSSSRGEV LLERIAAQAK QSGLSKLFVL TTRSIHWFQE RGFTPVDIDL 430 440 LPESKKQLYN YQRKSKVLMA DLG

References

[1]

"Genome sequence of enterohaemorrhagic Escherichia coli O157:H7." Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J., Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G., Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J., Davis N.W. , Lim A., Dimalanta E.T., Potamousis K., Apodaca J., Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A., Blattner F.R. Nature 409:529-533(2001) [PubMed: 11206551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / EDL933 / ATCC 700927 / EHEC.

[2]

"Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12." Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K., Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T., Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T. , Kuhara S., Shiba T., Hattori M., Shinagawa H. DNA Res. 8:11-22(2001) [PubMed: 11258796] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: O157:H7 / Sakai / RIMD 0509952 / EHEC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE005174 Genomic DNA. Translation: AAG57929.1. BA000007 Genomic DNA. Translation: BAB37098.1.
PIR	C91088. E85933.
RefSeq	NP_289370.1. NC_002655.2. NP_311702.1. NC_002695.1.
3D structure databases
ProteinModelPortal	P0A6C7.
SMR	P0A6C7. Positions 8-442.
ModBase	Search...
Proteomic databases
PRIDE	P0A6C7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000027119; EBESCP00000026012; EBESCG00000026171. EBESCT00000059088; EBESCP00000056916; EBESCG00000058136.
GeneID	916513. 957960.
GenomeReviews	Gene locus Z4135 in contig AE005174_GR. Gene locus ECs3675 in contig BA000007_GR.
KEGG	ece:Z4135. ecs:ECs3675.
PATRIC	18356837. VBIEscCol44059_3598.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000010674.
OMA	AFNLAME.
ProtClustDB	PRK05279.
Enzyme and pathway databases
BioCyc	ECOL83334:ECS3675-MONOMER.
Family and domain databases
HAMAP	MF_01105. N-acetyl_glu_synth. [Tree]
InterPro	IPR000182. AcTrfase_GCN5-related_dom. IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
KO	K14682.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
SUPFAM	SSF53633. Aa_kinase. 1 hit. SSF55729. Acyl_CoA_acyltransferase. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_ECO57

Accession

Primary (citable) accession number: P0A6C7
Secondary accession number(s): O68009 , O68010, O68011, O68012, O68013, P08205

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program