Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Amino-acid acetyltransferase

Gene

argA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate.1 Publication

Enzyme regulationi

Feedback inhibition by L-arginine.1 Publication

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate.1 Publication
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Amino-acid acetyltransferase (argA)
  2. Acetylglutamate kinase (argB)
  3. N-acetyl-gamma-glutamyl-phosphate reductase (argC)
  4. Acetylornithine/succinyldiaminopimelate aminotransferase (argD)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(2)-acetyl-L-ornithine from L-glutamate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

GO - Biological processi

  • arginine biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:N-ACETYLTRANSFER-MONOMER.
ECOL316407:JW2786-MONOMER.
MetaCyc:N-ACETYLTRANSFER-MONOMER.
BRENDAi2.3.1.1. 2026.
UniPathwayiUPA00068; UER00106.

Names & Taxonomyi

Protein namesi
Recommended name:
Amino-acid acetyltransferase (EC:2.3.1.1)
Alternative name(s):
N-acetylglutamate synthase
Short name:
AGS
Short name:
NAGS
Gene namesi
Name:argA
Ordered Locus Names:b2818, JW2786
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10063. argA.

Subcellular locationi

  • Cytoplasm 1 Publication

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi15 – 151H → Y in EE17. 1 Publication
Mutagenesisi19 – 191Y → C in EE51. 1 Publication
Mutagenesisi54 – 541S → N in PT2M217. 1 Publication
Mutagenesisi58 – 581R → H in EE11. 1 Publication
Mutagenesisi287 – 2871G → S in PT2M216. 1 Publication
Mutagenesisi432 – 4321Q → R in PT2M217. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 443443Amino-acid acetyltransferasePRO_0000186790Add
BLAST

Proteomic databases

PaxDbiP0A6C5.
PRIDEiP0A6C5.

Interactioni

Subunit structurei

Homohexamer.1 Publication

Protein-protein interaction databases

BioGridi4262306. 11 interactions.
DIPiDIP-48037N.
IntActiP0A6C5. 3 interactions.
MINTiMINT-1292378.
STRINGi511145.b2818.

Structurei

3D structure databases

ProteinModelPortaliP0A6C5.
SMRiP0A6C5. Positions 9-442.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini296 – 443148N-acetyltransferaseAdd
BLAST

Sequence similaritiesi

Contains 1 N-acetyltransferase domain.Curated

Phylogenomic databases

eggNOGiENOG4108EKC. Bacteria.
COG0548. LUCA.
COG1246. LUCA.
InParanoidiP0A6C5.
KOiK14682.
OMAiKQALYNY.
PhylomeDBiP0A6C5.

Family and domain databases

CDDicd04237. AAK_NAGS-ABP. 1 hit.
Gene3Di3.40.1160.10. 1 hit.
3.40.630.30. 1 hit.
HAMAPiMF_01105. N_acetyl_glu_synth. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000182. GNAT_dom.
IPR033719. NAGS_kin.
IPR010167. NH2A_AcTrfase.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000423. ArgA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6C5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKERKTELV EGFRHSVPYI NTHRGKTFVI MLGGEAIEHE NFSSIVNDIG
60 70 80 90 100
LLHSLGIRLV VVYGARPQID ANLAAHHHEP LYHKNIRVTD AKTLELVKQA
110 120 130 140 150
AGTLQLDITA RLSMSLNNTP LQGAHINVVS GNFIIAQPLG VDDGVDYCHS
160 170 180 190 200
GRIRRIDEDA IHRQLDSGAI VLMGPVAVSV TGESFNLTSE EIATQLAIKL
210 220 230 240 250
KAEKMIGFCS SQGVTNDDGD IVSELFPNEA QARVEAQEEK GDYNSGTVRF
260 270 280 290 300
LRGAVKACRS GVRRCHLISY QEDGALLQEL FSRDGIGTQI VMESAEQIRR
310 320 330 340 350
ATINDIGGIL ELIRPLEQQG ILVRRSREQL EMEIDKFTII QRDNTTIACA
360 370 380 390 400
ALYPFPEEKI GEMACVAVHP DYRSSSRGEV LLERIAAQAK QSGLSKLFVL
410 420 430 440
TTRSIHWFQE RGFTPVDIDL LPESKKQLYN YQRKSKVLMA DLG
Length:443
Mass (Da):49,195
Last modified:August 1, 1988 - v1
Checksum:iAE0D84902456797A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00492 Genomic DNA. Translation: CAA68547.1.
AF008115 Genomic DNA. Translation: AAC23443.1.
AF008116 Genomic DNA. Translation: AAC23444.1.
AF008117 Genomic DNA. Translation: AAC23445.1.
AF008118 Genomic DNA. Translation: AAC23446.1.
AF008119 Genomic DNA. Translation: AAC23447.1.
U29581 Genomic DNA. Translation: AAB40465.1.
U00096 Genomic DNA. Translation: AAC75857.1.
AP009048 Genomic DNA. Translation: BAE76887.1.
PIRiA30372. XYECAA.
RefSeqiNP_417295.1. NC_000913.3.
WP_000237947.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75857; AAC75857; b2818.
BAE76887; BAE76887; BAE76887.
GeneIDi947289.
KEGGiecj:JW2786.
eco:b2818.
PATRICi32121054. VBIEscCol129921_2916.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00492 Genomic DNA. Translation: CAA68547.1.
AF008115 Genomic DNA. Translation: AAC23443.1.
AF008116 Genomic DNA. Translation: AAC23444.1.
AF008117 Genomic DNA. Translation: AAC23445.1.
AF008118 Genomic DNA. Translation: AAC23446.1.
AF008119 Genomic DNA. Translation: AAC23447.1.
U29581 Genomic DNA. Translation: AAB40465.1.
U00096 Genomic DNA. Translation: AAC75857.1.
AP009048 Genomic DNA. Translation: BAE76887.1.
PIRiA30372. XYECAA.
RefSeqiNP_417295.1. NC_000913.3.
WP_000237947.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A6C5.
SMRiP0A6C5. Positions 9-442.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262306. 11 interactions.
DIPiDIP-48037N.
IntActiP0A6C5. 3 interactions.
MINTiMINT-1292378.
STRINGi511145.b2818.

Proteomic databases

PaxDbiP0A6C5.
PRIDEiP0A6C5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75857; AAC75857; b2818.
BAE76887; BAE76887; BAE76887.
GeneIDi947289.
KEGGiecj:JW2786.
eco:b2818.
PATRICi32121054. VBIEscCol129921_2916.

Organism-specific databases

EchoBASEiEB0061.
EcoGeneiEG10063. argA.

Phylogenomic databases

eggNOGiENOG4108EKC. Bacteria.
COG0548. LUCA.
COG1246. LUCA.
InParanoidiP0A6C5.
KOiK14682.
OMAiKQALYNY.
PhylomeDBiP0A6C5.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00106.
BioCyciEcoCyc:N-ACETYLTRANSFER-MONOMER.
ECOL316407:JW2786-MONOMER.
MetaCyc:N-ACETYLTRANSFER-MONOMER.
BRENDAi2.3.1.1. 2026.

Miscellaneous databases

PROiP0A6C5.

Family and domain databases

CDDicd04237. AAK_NAGS-ABP. 1 hit.
Gene3Di3.40.1160.10. 1 hit.
3.40.630.30. 1 hit.
HAMAPiMF_01105. N_acetyl_glu_synth. 1 hit.
InterProiIPR016181. Acyl_CoA_acyltransferase.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR000182. GNAT_dom.
IPR033719. NAGS_kin.
IPR010167. NH2A_AcTrfase.
[Graphical view]
PfamiPF00696. AA_kinase. 1 hit.
PF00583. Acetyltransf_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000423. ArgA. 1 hit.
SUPFAMiSSF53633. SSF53633. 1 hit.
SSF55729. SSF55729. 1 hit.
TIGRFAMsiTIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITEiPS51186. GNAT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARGA_ECOLI
AccessioniPrimary (citable) accession number: P0A6C5
Secondary accession number(s): O68009
, O68010, O68011, O68012, O68013, P08205, Q2MA19
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

The mutant strains shown below are all feedback-resistant (fbr) strains exhibiting enhanced arginine biosynthesis.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.