Reviewed, UniProtKB/Swiss-Prot P0A6C5 (ARGA_ECOLI)

Names and origin

Protein names

Recommended name:
    Amino-acid acetyltransferase
    EC=2.3.1.1
Alternative name(s):
    N-acetylglutamate synthase
      Short name=AGS
      Short name=NAGS

Gene names

Name:	argA
Ordered Locus Names:	b2818, JW2786

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	443 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + L-glutamate = CoA + N-acetyl-L-glutamate. HAMAP MF_01105
Enzyme regulation	Feedback inhibition by L-arginine. HAMAP MF_01105
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 1/4. HAMAP MF_01105
Subunit structure	Homohexamer Probable.
Subcellular location	Cytoplasm Probable.
Miscellaneous	The mutant strains shown below are all feedback-resistant (fbr) strains exhibiting enhanced arginine biosynthesis. HAMAP MF_01105
Sequence similarities	Belongs to the acetyltransferase family. ArgA subfamily. Contains 1 N-acetyltransferase domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 443	443	Amino-acid acetyltransferase HAMAP MF_01105		PRO_0000186790
Regions
	Domain	296 – 443	148	N-acetyltransferase
Experimental info
	Mutagenesis	15	1	H → Y in EE17. Ref.2
	Mutagenesis	19	1	Y → C in EE51. Ref.2
	Mutagenesis	54	1	S → N in PT2M217. Ref.2
	Mutagenesis	58	1	R → H in EE11. Ref.2
	Mutagenesis	287	1	G → S in PT2M216. Ref.2
	Mutagenesis	432	1	Q → R in PT2M217. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A6C5-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: AE0D84902456797A	FASTA	443	49,195
	10 20 30 40 50 60 MVKERKTELV EGFRHSVPYI NTHRGKTFVI MLGGEAIEHE NFSSIVNDIG LLHSLGIRLV 70 80 90 100 110 120 VVYGARPQID ANLAAHHHEP LYHKNIRVTD AKTLELVKQA AGTLQLDITA RLSMSLNNTP 130 140 150 160 170 180 LQGAHINVVS GNFIIAQPLG VDDGVDYCHS GRIRRIDEDA IHRQLDSGAI VLMGPVAVSV 190 200 210 220 230 240 TGESFNLTSE EIATQLAIKL KAEKMIGFCS SQGVTNDDGD IVSELFPNEA QARVEAQEEK 250 260 270 280 290 300 GDYNSGTVRF LRGAVKACRS GVRRCHLISY QEDGALLQEL FSRDGIGTQI VMESAEQIRR 310 320 330 340 350 360 ATINDIGGIL ELIRPLEQQG ILVRRSREQL EMEIDKFTII QRDNTTIACA ALYPFPEEKI 370 380 390 400 410 420 GEMACVAVHP DYRSSSRGEV LLERIAAQAK QSGLSKLFVL TTRSIHWFQE RGFTPVDIDL 430 440 LPESKKQLYN YQRKSKVLMA DLG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of the Escherichia coli argA gene." Brown K., Finch P.W., Hickson I.D., Emmerson P.T. Nucleic Acids Res. 15:10586-10586(1987) [PubMed: 3320971] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Use of inducible feedback-resistant N-acetylglutamate synthetase (argA) genes for enhanced arginine biosynthesis by genetically engineered Escherichia coli K-12 strains." Rajagopal B.S., Deponte J. III, Tuchman M., Malamy M.H. Appl. Environ. Microbiol. 64:1805-1811(1998) [PubMed: 9572954] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF HIS-15; TYR-19; SER-54; ARG-58; GLY-287 AND GLN-432. Strain: K12 / Various isolates.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"N-acetylglutamate synthase of Escherichia coli: purification, characterization, and molecular properties." Marvil D.K., Leisinger T. J. Biol. Chem. 252:3295-3303(1977) [PubMed: 16890] [Abstract] Cited for: CHARACTERIZATION. Strain: K12.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00492 Genomic DNA. Translation: CAA68547.1. AF008115 Genomic DNA. Translation: AAC23443.1. AF008116 Genomic DNA. Translation: AAC23444.1. AF008117 Genomic DNA. Translation: AAC23445.1. AF008118 Genomic DNA. Translation: AAC23446.1. AF008119 Genomic DNA. Translation: AAC23447.1. U29581 Genomic DNA. Translation: AAB40465.1. U00096 Genomic DNA. Translation: AAC75857.1. AP009048 Genomic DNA. Translation: BAE76887.1.
PIR	XYECAA. A30372.
RefSeq	AP_003381.1. NP_417295.1.
3D structure databases
ModBase	Search...
2-D gel databases
ECO2DBASE	H052.7. 6TH EDITION.
Genome annotation databases
GeneID	947289.
GenomeReviews	Gene locus JW2786 in contig AP009048_GR. Gene locus b2818 in contig U00096_GR.
KEGG	ecj:JW2786. eco:b2818.
Organism-specific databases
EchoBASE	EB0061.
EcoGene	EG10063. argA.
CMR	Search...
Phylogenomic databases
OMA	P0A6C5. DLIRPLE.
Enzyme and pathway databases
BioCyc	EcoCyc:N-ACETYLTRANSFER-MON. MetaCyc:N-ACETYLTRANSFER-MON.
Family and domain databases
HAMAP	MF_01105. [Tree]
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR001048. Asp/Glu/Uridylate_kinase. IPR000182. GCN5-rel_AcTrfase. IPR010167. NH2A_AcTrfase_ArgA. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF00583. Acetyltransf_1. 1 hit. [Graphical view]
PIRSF	PIRSF000423. ArgA. 1 hit.
TIGRFAMs	TIGR01890. N-Ac-Glu-synth. 1 hit.
PROSITE	PS51186. GNAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGA_ECOLI

Accession

Primary (citable) accession number: P0A6C5
Secondary accession number(s): O68009 , O68010, O68011, O68012, O68013, P08205, Q2MA19

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)