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Protein

Endonuclease 4

Gene

nfo

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.

Cofactori

Zn2+By similarityNote: Binds 3 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Zinc 1By similarity1
Metal bindingi109Zinc 1By similarity1
Metal bindingi145Zinc 1By similarity1
Metal bindingi145Zinc 2By similarity1
Metal bindingi179Zinc 2By similarity1
Metal bindingi182Zinc 3By similarity1
Metal bindingi216Zinc 2By similarity1
Metal bindingi229Zinc 3By similarity1
Metal bindingi231Zinc 3By similarity1
Metal bindingi261Zinc 2By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Manganese, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciECOO157:NFO-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 4 (EC:3.1.21.2)
Alternative name(s):
Endodeoxyribonuclease IV
Endonuclease IV
Gene namesi
Name:nfo
Ordered Locus Names:Z3416, ECs3051
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908391 – 285Endonuclease 4Add BLAST285

Proteomic databases

PRIDEiP0A6C2.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

MINTiMINT-1242687.
STRINGi155864.Z3416.

Structurei

3D structure databases

ProteinModelPortaliP0A6C2.
SMRiP0A6C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224893.
KOiK01151.
OMAiMKYVGAH.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYIGAHVSA AGGLANAAIR AAEIDATAFA LFTKNQRQWR AAPLTTQTID
60 70 80 90 100
EFKAACEKYH YTSAQILPHD SYLINLGHPV TEALEKSRDA FIDEMQRCEQ
110 120 130 140 150
LGLSLLNFHP GSHLMQISEE DCLARIAESI NIALDKTQGV TAVIENTAGQ
160 170 180 190 200
GSNLGFKFEH LAAIIDGVED KSRVGVCIDT CHAFAAGYDL RTPAECEKTF
210 220 230 240 250
ADFARTVGFK YLRGMHLNDA KSTFGSRVDR HHSLGEGNIG HDAFRWIMQD
260 270 280
DRFDGIPLIL ETINPDIWAE EIAWLKAQQT EKAVA
Length:285
Mass (Da):31,480
Last modified:June 7, 2005 - v1
Checksum:iEAAE5861E54C47FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57297.1.
BA000007 Genomic DNA. Translation: BAB36474.1.
PIRiC91010.
RefSeqiNP_311078.1. NC_002695.1.
WP_000873894.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG57297; AAG57297; Z3416.
BAB36474; BAB36474; BAB36474.
GeneIDi916755.
KEGGiece:Z3416.
ecs:ECs3051.
PATRICi18355474. VBIEscCol44059_2939.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57297.1.
BA000007 Genomic DNA. Translation: BAB36474.1.
PIRiC91010.
RefSeqiNP_311078.1. NC_002695.1.
WP_000873894.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP0A6C2.
SMRiP0A6C2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-1242687.
STRINGi155864.Z3416.

Proteomic databases

PRIDEiP0A6C2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG57297; AAG57297; Z3416.
BAB36474; BAB36474; BAB36474.
GeneIDi916755.
KEGGiece:Z3416.
ecs:ECs3051.
PATRICi18355474. VBIEscCol44059_2939.

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224893.
KOiK01151.
OMAiMKYVGAH.

Enzyme and pathway databases

BioCyciECOO157:NFO-MONOMER.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEND4_ECO57
AccessioniPrimary (citable) accession number: P0A6C2
Secondary accession number(s): P12638, P78086
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.