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Protein

Endonuclease 4

Gene

nfo

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin (By similarity).By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.

Cofactori

Zn2+By similarityNote: Binds 3 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi69Zinc 1By similarity1
Metal bindingi109Zinc 1By similarity1
Metal bindingi145Zinc 1By similarity1
Metal bindingi145Zinc 2By similarity1
Metal bindingi179Zinc 2By similarity1
Metal bindingi182Zinc 3By similarity1
Metal bindingi216Zinc 2By similarity1
Metal bindingi229Zinc 3By similarity1
Metal bindingi231Zinc 3By similarity1
Metal bindingi261Zinc 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processDNA damage, DNA repair
LigandManganese, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease 4 (EC:3.1.21.2)
Alternative name(s):
Endodeoxyribonuclease IV
Endonuclease IV
Gene namesi
Name:nfo
Ordered Locus Names:Z3416, ECs3051
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001908391 – 285Endonuclease 4Add BLAST285

Proteomic databases

PRIDEiP0A6C2.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

MINTiMINT-1242687.
STRINGi155864.Z3416.

Structurei

3D structure databases

ProteinModelPortaliP0A6C2.
SMRiP0A6C2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.Curated

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224893.
KOiK01151.

Family and domain databases

CDDicd00019. AP2Ec. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiView protein in InterPro
IPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR036237. Xyl_isomerase-like_sf.
IPR013022. Xyl_isomerase-like_TIM-brl.
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiView protein in Pfam
PF01261. AP_endonuc_2. 1 hit.
SMARTiView protein in SMART
SM00518. AP2Ec. 1 hit.
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiView protein in PROSITE
PS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6C2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKYIGAHVSA AGGLANAAIR AAEIDATAFA LFTKNQRQWR AAPLTTQTID
60 70 80 90 100
EFKAACEKYH YTSAQILPHD SYLINLGHPV TEALEKSRDA FIDEMQRCEQ
110 120 130 140 150
LGLSLLNFHP GSHLMQISEE DCLARIAESI NIALDKTQGV TAVIENTAGQ
160 170 180 190 200
GSNLGFKFEH LAAIIDGVED KSRVGVCIDT CHAFAAGYDL RTPAECEKTF
210 220 230 240 250
ADFARTVGFK YLRGMHLNDA KSTFGSRVDR HHSLGEGNIG HDAFRWIMQD
260 270 280
DRFDGIPLIL ETINPDIWAE EIAWLKAQQT EKAVA
Length:285
Mass (Da):31,480
Last modified:June 7, 2005 - v1
Checksum:iEAAE5861E54C47FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG57297.1.
BA000007 Genomic DNA. Translation: BAB36474.1.
PIRiC91010.
RefSeqiNP_311078.1. NC_002695.1.
WP_000873894.1. NZ_NOKN01000002.1.

Genome annotation databases

EnsemblBacteriaiAAG57297; AAG57297; Z3416.
BAB36474; BAB36474; BAB36474.
GeneIDi916755.
KEGGiece:Z3416.
ecs:ECs3051.
PATRICifig|386585.9.peg.3180.

Similar proteinsi

Entry informationi

Entry nameiEND4_ECO57
AccessioniPrimary (citable) accession number: P0A6C2
Secondary accession number(s): P12638, P78086
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 22, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families