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P0A6B5

- ALR1_SHIFL

UniProt

P0A6B5 - ALR1_SHIFL

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Protein
Alanine racemase, biosynthetic
Gene
alr, SF4152, S3578
Organism
Shigella flexneri
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the interconversion of L-alanine and D-alanine. Provides the D-alanine required for cell wall biosynthesis.1 Publication

Catalytic activityi

L-alanine = D-alanine.1 Publication

Cofactori

Pyridoxal phosphate.1 Publication

pH dependencei

Optimum pH is 8-10.1 Publication

Temperature dependencei

Optimum temperature is 50 degrees Celsius.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341Proton acceptor; specific for D-alanine By similarity
Binding sitei129 – 1291Substrate By similarity
Active sitei255 – 2551Proton acceptor; specific for L-alanine By similarity
Binding sitei303 – 3031Substrate; via amide nitrogen By similarity

GO - Molecular functioni

  1. alanine racemase activity Source: UniProtKB-HAMAP
  2. pyridoxal phosphate binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. D-alanine biosynthetic process Source: UniProtKB-UniPathway
  2. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
  3. regulation of cell shape Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi5.1.1.1. 5712.
UniPathwayiUPA00042; UER00497.
UPA00219.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine racemase, biosynthetic (EC:5.1.1.1)
Gene namesi
Name:alr
Ordered Locus Names:SF4152, S3578
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella
ProteomesiUP000001006: Chromosome, UP000002673: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 359359Alanine racemase, biosyntheticUniRule annotation
PRO_0000114565Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341N6-(pyridoxal phosphate)lysine By similarity

Proteomic databases

PaxDbiP0A6B5.

Interactioni

Subunit structurei

Monomer but homodimer in the presence of the substrate.1 Publication

Protein-protein interaction databases

STRINGi198214.SF4152.

Structurei

3D structure databases

ProteinModelPortaliP0A6B5.
SMRiP0A6B5. Positions 1-359.

Family & Domainsi

Sequence similaritiesi

Belongs to the alanine racemase family.

Phylogenomic databases

eggNOGiCOG0787.
HOGENOMiHOG000031446.
KOiK01775.
OMAiLWQLEAI.
OrthoDBiEOG6PP9NJ.

Family and domain databases

Gene3Di2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPiMF_01201. Ala_racemase.
InterProiIPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view]
PfamiPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSiPR00992. ALARACEMASE.
SMARTiSM01005. Ala_racemase_C. 1 hit.
[Graphical view]
SUPFAMiSSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsiTIGR00492. alr. 1 hit.
PROSITEiPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6B5-1 [UniParc]FASTAAdd to Basket

« Hide

MQAATVVINR RALRHNLQRL RELAPASKMV AVVKANAYGH GLLETARTLP    50
DADAFGVARL EEALRLRAGG ITKPVLLLEG FFDARDLPTI SAQHFHTAVH 100
NEEQLAALEE ASLDEPVTVW MKLDTGMHRL GVRPEQAEAF YHRLTQCKNV 150
RQPVNIVSHF ARADEPKCGA TEKQLAIFNT FCEGKPGQRS IAASGGILLW 200
PQSHFDWVRP GIILYGVSPL EDRSTGADFG CQPVMSLTSS LIAVREHKAG 250
EPVGYGGTWV SERDTRLGVV AMGYGDGYPR AAPSGTPVLV NGREVPIVGR 300
VAMDMICVDL GPQAQDKAGD PVILWGEGLP VERIAEMTKV SAYELITRLT 350
SRVAMKYVD 359
Length:359
Mass (Da):39,153
Last modified:June 7, 2005 - v1
Checksum:iFDE9B438115342C2
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti138 – 1381E → G in BAB71772. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB070927 Genomic DNA. Translation: BAB71772.1.
AE005674 Genomic DNA. Translation: AAN45574.1.
AE014073 Genomic DNA. Translation: AAP18624.1.
RefSeqiNP_709867.1. NC_004337.2.
NP_838813.1. NC_004741.1.

Genome annotation databases

EnsemblBacteriaiAAN45574; AAN45574; SF4152.
AAP18624; AAP18624; S3578.
GeneIDi1025471.
1079800.
KEGGisfl:SF4152.
sfx:S3578.
PATRICi18708936. VBIShiFle31049_3906.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB070927 Genomic DNA. Translation: BAB71772.1 .
AE005674 Genomic DNA. Translation: AAN45574.1 .
AE014073 Genomic DNA. Translation: AAP18624.1 .
RefSeqi NP_709867.1. NC_004337.2.
NP_838813.1. NC_004741.1.

3D structure databases

ProteinModelPortali P0A6B5.
SMRi P0A6B5. Positions 1-359.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 198214.SF4152.

Chemistry

BindingDBi P0A6B5.

Proteomic databases

PaxDbi P0A6B5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN45574 ; AAN45574 ; SF4152 .
AAP18624 ; AAP18624 ; S3578 .
GeneIDi 1025471.
1079800.
KEGGi sfl:SF4152.
sfx:S3578.
PATRICi 18708936. VBIShiFle31049_3906.

Phylogenomic databases

eggNOGi COG0787.
HOGENOMi HOG000031446.
KOi K01775.
OMAi LWQLEAI.
OrthoDBi EOG6PP9NJ.

Enzyme and pathway databases

UniPathwayi UPA00042 ; UER00497 .
UPA00219 .
BRENDAi 5.1.1.1. 5712.

Family and domain databases

Gene3Di 2.40.37.10. 1 hit.
3.20.20.10. 1 hit.
HAMAPi MF_01201. Ala_racemase.
InterProi IPR000821. Ala_racemase.
IPR009006. Ala_racemase/Decarboxylase_C.
IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR020622. Ala_racemase_pyridoxalP-BS.
IPR029066. PLP-binding_barrel.
[Graphical view ]
Pfami PF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view ]
PRINTSi PR00992. ALARACEMASE.
SMARTi SM01005. Ala_racemase_C. 1 hit.
[Graphical view ]
SUPFAMi SSF50621. SSF50621. 1 hit.
SSF51419. SSF51419. 1 hit.
TIGRFAMsi TIGR00492. alr. 1 hit.
PROSITEi PS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Gene cloning and characterization of alanine racemases from Shigella dysenteriae, Shigella boydii, Shigella flexneri, and Shigella sonnei."
    Yokoigawa K., Hirasawa R., Ueno H., Okubo Y., Umesako S., Soda K.
    Biochem. Biophys. Res. Commun. 288:676-684(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-21, FUNCTION, CATALYTIC ACTIVITY, COFACTOR, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT.
  2. "Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
    Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y.
    , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
    Nucleic Acids Res. 30:4432-4441(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 301 / Serotype 2a.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700930 / 2457T / Serotype 2a.

Entry informationi

Entry nameiALR1_SHIFL
AccessioniPrimary (citable) accession number: P0A6B5
Secondary accession number(s): P29743, P78136
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: June 11, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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