Alanine racemase, biosynthetic - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A6B4 (ALR1_ECOLI)

Last modified June 16, 2009. Version 45. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
Alanine racemase, biosynthetic
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	b4053, JW4013

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	359 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity.
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

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Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: HAMAP cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 359

359

Alanine racemase, biosynthetic HAMAP MF_01201

PRO_0000114514

Sites

Active site

Proton acceptor; specific for D-alanine By similarity

Active site

255

Proton acceptor; specific for L-alanine By similarity

Amino acid modifications

Modified residue

N6-(pyridoxal phosphate)lysine By similarity

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A6B4-1 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: FDE9B438115342C2
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FASTA

359

39,153

        10         20         30         40         50         60 
MQAATVVINR RALRHNLQRL RELAPASKMV AVVKANAYGH GLLETARTLP DADAFGVARL 

        70         80         90        100        110        120 
EEALRLRAGG ITKPVLLLEG FFDARDLPTI SAQHFHTAVH NEEQLAALEE ASLDEPVTVW 

       130        140        150        160        170        180 
MKLDTGMHRL GVRPEQAEAF YHRLTQCKNV RQPVNIVSHF ARADEPKCGA TEKQLAIFNT 

       190        200        210        220        230        240 
FCEGKPGQRS IAASGGILLW PQSHFDWVRP GIILYGVSPL EDRSTGADFG CQPVMSLTSS 

       250        260        270        280        290        300 
LIAVREHKAG EPVGYGGTWV SERDTRLGVV AMGYGDGYPR AAPSGTPVLV NGREVPIVGR 

       310        320        330        340        350 
VAMDMICVDL GPQAQDKAGD PVILWGEGLP VERIAEMTKV SAYELITRLT SRVAMKYVD

« Hide

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The 92-min region of the Escherichia coli chromosome: location and cloning of the ubiA and alr genes." Lilley P.E., Stamford N.P., Vasudevan S.G., Dixon N.E. Gene 129:9-16(1993) [PubMed: 8335265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L. Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli." Nakayama N., Arai N., Bond M.W., Kaziro Y., Arai K. J. Biol. Chem. 259:97-101(1984) [PubMed: 6323420] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
[6]	"Aromatic amino acid aminotransferase of Escherichia coli: nucleotide sequence of the tyrB gene." Kuramitsu S., Inoue K., Ogawa T., Ogawa H., Kagamiyama H. Biochem. Biophys. Res. Commun. 133:134-139(1985) [PubMed: 3907634] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 320-359. Strain: K12.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

U00006 Genomic DNA. Translation: AAC43147.1.
U00096 Genomic DNA. Translation: AAC77023.1.
AP009048 Genomic DNA. Translation: BAE78055.1.
K01174 Genomic DNA. No translation available.
M12047 Genomic DNA. No translation available.

PIR

PC1296. D65213.

RefSeq

AP_004554.1.
NP_418477.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2RJG	X-ray	2.40	A/B/C/D	1-359	[»]
2RJH	X-ray	2.40	A/B/C/D	1-359	[»]
3B8T	X-ray	3.00	A/B/C/D	1-359	[»]
3B8U	X-ray	3.00	A/B/C/D	1-359	[»]
3B8V	X-ray	2.60	A/B/C/D	1-359	[»]
3B8W	X-ray	2.70	A/B/C/D	1-359	[»]

ModBase

Search...

Genome annotation databases

GeneID

948564.

GenomeReviews

Gene locus JW4013 in contig AP009048_GR.
Gene locus b4053 in contig U00096_GR.

KEGG

ecj:JW4013.
eco:b4053.

Organism-specific databases

EchoBASE

EB0001.

EcoGene

EG10001. alr.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A6B4.

OMA

P0A6B4. EGTPVWV.

Enzyme and pathway databases

BioCyc

EcoCyc:ALARACEBIOSYN-MON.
MetaCyc:ALARACEBIOSYN-MON.

Family and domain databases

HAMAP

MF_01201.
[Tree]

InterPro

IPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR000821. Ala_racemase_reg.
[Graphical view]

Pfam

PF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]

PRINTS

PR00992. ALARACEMASE.

TIGRFAMs

TIGR00492. alr. 1 hit.

PROSITE

PS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

ALR1_ECOLI

Accession

Primary (citable) accession number: P0A6B4
Secondary accession number(s): P29743, P78136, Q2M6Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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