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Reviewed, UniProtKB/Swiss-Prot P0A6B4 (ALR1_ECOLI)

Last modified June 16, 2009. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Alanine racemase, biosynthetic
    EC=5.1.1.1
Gene names
Name: alr
Ordered Locus Names: b4053, JW4013
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length359 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Provides the D-alanine required for cell wall biosynthesis By similarity.

Catalytic activity

L-alanine = D-alanine. HAMAP MF_01201

Cofactor

Pyridoxal phosphate By similarity.

Pathway

Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201

Sequence similarities

Belongs to the alanine racemase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 359359Alanine racemase, biosynthetic HAMAP MF_01201
PRO_0000114514

Sites

Active site341Proton acceptor; specific for D-alanine By similarity
Active site2551Proton acceptor; specific for L-alanine By similarity

Amino acid modifications

Modified residue341N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A6B4-1 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: FDE9B438115342C2

FASTA35939,153
        10         20         30         40         50         60 
MQAATVVINR RALRHNLQRL RELAPASKMV AVVKANAYGH GLLETARTLP DADAFGVARL 

        70         80         90        100        110        120 
EEALRLRAGG ITKPVLLLEG FFDARDLPTI SAQHFHTAVH NEEQLAALEE ASLDEPVTVW 

       130        140        150        160        170        180 
MKLDTGMHRL GVRPEQAEAF YHRLTQCKNV RQPVNIVSHF ARADEPKCGA TEKQLAIFNT 

       190        200        210        220        230        240 
FCEGKPGQRS IAASGGILLW PQSHFDWVRP GIILYGVSPL EDRSTGADFG CQPVMSLTSS 

       250        260        270        280        290        300 
LIAVREHKAG EPVGYGGTWV SERDTRLGVV AMGYGDGYPR AAPSGTPVLV NGREVPIVGR 

       310        320        330        340        350 
VAMDMICVDL GPQAQDKAGD PVILWGEGLP VERIAEMTKV SAYELITRLT SRVAMKYVD 

« Hide

References

« Hide 'large scale' references
[1]"The 92-min region of the Escherichia coli chromosome: location and cloning of the ubiA and alr genes."
Lilley P.E., Stamford N.P., Vasudevan S.G., Dixon N.E.
Gene 129:9-16(1993) [PubMed: 8335265] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes."
Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.
Nucleic Acids Res. 21:5408-5417(1993) [PubMed: 8265357] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Nucleotide sequence of dnaB and the primary structure of the dnaB protein from Escherichia coli."
Nakayama N., Arai N., Bond M.W., Kaziro Y., Arai K.
J. Biol. Chem. 259:97-101(1984) [PubMed: 6323420] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-22.
[6]"Aromatic amino acid aminotransferase of Escherichia coli: nucleotide sequence of the tyrB gene."
Kuramitsu S., Inoue K., Ogawa T., Ogawa H., Kagamiyama H.
Biochem. Biophys. Res. Commun. 133:134-139(1985) [PubMed: 3907634] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 320-359.
Strain: K12.
+Additional computationally mapped references.

Cross-references

Sequence databases

U00006 Genomic DNA. Translation: AAC43147.1.
U00096 Genomic DNA. Translation: AAC77023.1.
AP009048 Genomic DNA. Translation: BAE78055.1.
K01174 Genomic DNA. No translation available.
M12047 Genomic DNA. No translation available.
PIRPC1296. D65213.
RefSeqAP_004554.1.
NP_418477.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
2RJGX-ray2.40A/B/C/D1-359[»]
2RJHX-ray2.40A/B/C/D1-359[»]
3B8TX-ray3.00A/B/C/D1-359[»]
3B8UX-ray3.00A/B/C/D1-359[»]
3B8VX-ray2.60A/B/C/D1-359[»]
3B8WX-ray2.70A/B/C/D1-359[»]
ModBaseSearch...

Genome annotation databases

GeneID948564.
GenomeReviewsGene locus JW4013 in contig AP009048_GR.
Gene locus b4053 in contig U00096_GR.
KEGGecj:JW4013.
eco:b4053.

Organism-specific databases

EchoBASEEB0001.
EcoGeneEG10001. alr.
CMRSearch...

Phylogenomic databases

HOGENOMP0A6B4.
OMAP0A6B4. EGTPVWV.

Enzyme and pathway databases

BioCycEcoCyc:ALARACEBIOSYN-MON.
MetaCyc:ALARACEBIOSYN-MON.

Family and domain databases

HAMAPMF_01201.
[Tree]
InterProIPR011079. Ala_racemase_C.
IPR001608. Ala_racemase_N.
IPR000821. Ala_racemase_reg.
[Graphical view]
PfamPF00842. Ala_racemase_C. 1 hit.
PF01168. Ala_racemase_N. 1 hit.
[Graphical view]
PRINTSPR00992. ALARACEMASE.
TIGRFAMsTIGR00492. alr. 1 hit.
PROSITEPS00395. ALANINE_RACEMASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameALR1_ECOLI
AccessionPrimary (citable) accession number: P0A6B4
Secondary accession number(s): P29743, P78136, Q2M6Q1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents