Acetate kinase - Escherichia coli (strain K12)

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Reviewed, UniProtKB/Swiss-Prot P0A6A3 (ACKA_ECOLI)

Last modified June 16, 2009. Version 40. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Acetate kinase
    EC=2.7.2.1
Alternative name(s):
    Acetokinase

Gene names

Name:	ackA
Synonyms:	ack
Ordered Locus Names:	b2296, JW2293

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

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Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Involved in the activation of acetate to acetyl CoA and the secretion of acetate. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically. HAMAP MF_00020
Catalytic activity	ATP + acetate = ADP + acetyl phosphate. HAMAP MF_00020 ATP + propanoate = ADP + propanoyl phosphate. HAMAP MF_00020
Pathway	Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from acetate: step 1/2. HAMAP MF_00020
Subunit structure	Homodimer. HAMAP MF_00020
Subcellular location	Cytoplasm. HAMAP MF_00020
Sequence similarities	Belongs to the acetokinase family.

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Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	organic acid metabolic process Inferred from electronic annotation. Source: InterPro phosphorylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytosol Inferred from direct assay. Source: UniProtKB membrane Inferred from direct assay. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 400

400

Acetate kinase HAMAP MF_00020

PRO_0000107557

Experimental info

Sequence conflict

221

R → G in BAA04501. Ref.2

Sequence conflict

240

M → I in BAA04501. Ref.2

Secondary structure

400

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P0A6A3-1 [UniParc].

Last modified June 7, 2005. Version 1.
Checksum: 709C855C514656C8
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FASTA

400

43,290

        10         20         30         40         50         60 
MSSKLVLVLN CGSSSLKFAI IDAVNGEEYL SGLAECFHLP EARIKWKMDG NKQEAALGAG 

        70         80         90        100        110        120 
AAHSEALNFI VNTILAQKPE LSAQLTAIGH RIVHGGEKYT SSVVIDESVI QGIKDAASFA 

       130        140        150        160        170        180 
PLHNPAHLIG IEEALKSFPQ LKDKNVAVFD TAFHQTMPEE SYLYALPYNL YKEHGIRRYG 

       190        200        210        220        230        240 
AHGTSHFYVT QEAAKMLNKP VEELNIITCH LGNGGSVSAI RNGKCVDTSM GLTPLEGLVM 

       250        260        270        280        290        300 
GTRSGDIDPA IIFHLHDTLG MSVDAINKLL TKESGLLGLT EVTSDCRYVE DNYATKEDAK 

       310        320        330        340        350        360 
RAMDVYCHRL AKYIGAYTAL MDGRLDAVVF TGGIGENAAM VRELSLGKLG VLGFEVDHER 

       370        380        390        400 
NLAARFGKSG FINKEGTRPA VVIPTNEELV IAQDASRLTA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene." Matsuyama A., Yamamoto H., Nakano E. J. Bacteriol. 171:577-580(1989) [PubMed: 2536666] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE. Strain: K12.
[2]	"Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli." Kakuda H., Hosono K., Shiroishi K., Ichihara S. J. Biochem. 116:916-922(1994) [PubMed: 7883769] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / KH131.
[3]	"Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T. Horiuchi T. DNA Res. 4:91-113(1997) [PubMed: 9205837] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.

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Cross-references

Sequence databases

M22956 Genomic DNA. Translation: AAA23406.1.
D17576 Genomic DNA. Translation: BAA04501.1.
U00096 Genomic DNA. Translation: AAC75356.1.
AP009048 Genomic DNA. Translation: BAA16135.1.

PIR

KIECAA. JT0498.

RefSeq

AP_002896.1.
NP_416799.1.

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LRG	model	-	A	1-400	[»]

ModBase

Search...

2-D gel databases

SWISS-2DPAGE

P0A6A3.

ECO2DBASE

G041.3. 6TH EDITION.

Genome annotation databases

GeneID

946775.

GenomeReviews

Gene locus JW2293 in contig AP009048_GR.
Gene locus b2296 in contig U00096_GR.

KEGG

ecj:JW2293.
eco:b2296.

Organism-specific databases

EchoBASE

EB0026.

EcoGene

EG10027. ackA.

CMR

Search...

Phylogenomic databases

HOGENOM

P0A6A3.

OMA

P0A6A3. PHLKDKN.

Enzyme and pathway databases

BioCyc

EcoCyc:ACETATEKINA-MON.
MetaCyc:ACETATEKINA-MON.

Family and domain databases

HAMAP

MF_00020.
[Tree]

InterPro

IPR000890. Acetate_kin.
IPR004372. AckA.
[Graphical view]

PANTHER

PTHR21060. Acetate_kin. 1 hit.
PTHR21060:SF11. AckA. 1 hit.

Pfam

PF00871. Acetate_kinase. 1 hit.
[Graphical view]

PIRSF

PIRSF000722. Acetate_prop_kin. 1 hit.

PRINTS

PR00471. ACETATEKNASE.

TIGRFAMs

TIGR00016. ackA. 1 hit.

PROSITE

PS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

ACKA_ECOLI

Accession

Primary (citable) accession number: P0A6A3
Secondary accession number(s): P15046, P78188, Q59386

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 7, 2005
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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