Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Acetate kinase

Gene

ackA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of acetyl phosphate from acetate and ATP. Can also catalyze the reverse reaction. During anaerobic growth of the organism, this enzyme is also involved in the synthesis of most of the ATP formed catabolically.

Catalytic activityi

ATP + acetate = ADP + acetyl phosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Mg2+. Can also accept Mn2+.UniRule annotation

Pathwayi: acetyl-CoA biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes acetyl-CoA from acetate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Acetate kinase (ackA)
  2. Phosphate acetyltransferase (pta)
This subpathway is part of the pathway acetyl-CoA biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes acetyl-CoA from acetate, the pathway acetyl-CoA biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi10MagnesiumUniRule annotation1
Binding sitei17ATPUniRule annotation1
Binding sitei91SubstrateUniRule annotation1
Active sitei150Proton donor/acceptorUniRule annotation1
Sitei182Transition state stabilizerUniRule annotation1
Sitei243Transition state stabilizerUniRule annotation1
Metal bindingi387MagnesiumUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi210 – 214ATPUniRule annotation5
Nucleotide bindingi285 – 287ATPUniRule annotation3
Nucleotide bindingi333 – 337ATPUniRule annotation5

GO - Molecular functioni

  • acetate kinase activity Source: EcoliWiki
  • ATP binding Source: UniProtKB-KW
  • magnesium ion binding Source: UniProtKB-HAMAP
  • zinc ion binding Source: EcoliWiki

GO - Biological processi

  • acetate biosynthetic process Source: EcoliWiki
  • acetate metabolic process Source: CACAO
  • acetyl-CoA biosynthetic process Source: UniProtKB-UniPathway
  • propionate biosynthetic process Source: EcoliWiki
  • single-species biofilm formation on inanimate substrate Source: CACAO
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ACETATEKINA-MONOMER.
ECOL316407:JW2293-MONOMER.
MetaCyc:ACETATEKINA-MONOMER.
SABIO-RKP0A6A3.
UniPathwayiUPA00340; UER00458.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetate kinaseUniRule annotation (EC:2.7.2.1UniRule annotation)
Alternative name(s):
AcetokinaseUniRule annotation
Gene namesi
Name:ackAUniRule annotation
Synonyms:ack
Ordered Locus Names:b2296, JW2293
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10027. ackA.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: UniProtKB
  • membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001075571 – 400Acetate kinaseAdd BLAST400

Proteomic databases

EPDiP0A6A3.
PaxDbiP0A6A3.
PRIDEiP0A6A3.

2D gel databases

SWISS-2DPAGEP0A6A3.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

BioGridi4261690. 452 interactors.
DIPiDIP-9042N.
IntActiP0A6A3. 12 interactors.
STRINGi511145.b2296.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LRGmodel-A1-400[»]
ProteinModelPortaliP0A6A3.
SMRiP0A6A3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the acetokinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288398.
InParanoidiP0A6A3.
KOiK00925.
OMAiHRLKKYI.
PhylomeDBiP0A6A3.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A6A3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSKLVLVLN CGSSSLKFAI IDAVNGEEYL SGLAECFHLP EARIKWKMDG
60 70 80 90 100
NKQEAALGAG AAHSEALNFI VNTILAQKPE LSAQLTAIGH RIVHGGEKYT
110 120 130 140 150
SSVVIDESVI QGIKDAASFA PLHNPAHLIG IEEALKSFPQ LKDKNVAVFD
160 170 180 190 200
TAFHQTMPEE SYLYALPYNL YKEHGIRRYG AHGTSHFYVT QEAAKMLNKP
210 220 230 240 250
VEELNIITCH LGNGGSVSAI RNGKCVDTSM GLTPLEGLVM GTRSGDIDPA
260 270 280 290 300
IIFHLHDTLG MSVDAINKLL TKESGLLGLT EVTSDCRYVE DNYATKEDAK
310 320 330 340 350
RAMDVYCHRL AKYIGAYTAL MDGRLDAVVF TGGIGENAAM VRELSLGKLG
360 370 380 390 400
VLGFEVDHER NLAARFGKSG FINKEGTRPA VVIPTNEELV IAQDASRLTA
Length:400
Mass (Da):43,290
Last modified:June 7, 2005 - v1
Checksum:i709C855C514656C8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti221R → G in BAA04501 (PubMed:7883769).Curated1
Sequence conflicti240M → I in BAA04501 (PubMed:7883769).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22956 Genomic DNA. Translation: AAA23406.1.
D17576 Genomic DNA. Translation: BAA04501.1.
U00096 Genomic DNA. Translation: AAC75356.1.
AP009048 Genomic DNA. Translation: BAA16135.1.
PIRiJT0498. KIECAA.
RefSeqiNP_416799.1. NC_000913.3.
WP_000095707.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75356; AAC75356; b2296.
BAA16135; BAA16135; BAA16135.
GeneIDi946775.
KEGGiecj:JW2293.
eco:b2296.
PATRICi32119963. VBIEscCol129921_2391.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22956 Genomic DNA. Translation: AAA23406.1.
D17576 Genomic DNA. Translation: BAA04501.1.
U00096 Genomic DNA. Translation: AAC75356.1.
AP009048 Genomic DNA. Translation: BAA16135.1.
PIRiJT0498. KIECAA.
RefSeqiNP_416799.1. NC_000913.3.
WP_000095707.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LRGmodel-A1-400[»]
ProteinModelPortaliP0A6A3.
SMRiP0A6A3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261690. 452 interactors.
DIPiDIP-9042N.
IntActiP0A6A3. 12 interactors.
STRINGi511145.b2296.

2D gel databases

SWISS-2DPAGEP0A6A3.

Proteomic databases

EPDiP0A6A3.
PaxDbiP0A6A3.
PRIDEiP0A6A3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75356; AAC75356; b2296.
BAA16135; BAA16135; BAA16135.
GeneIDi946775.
KEGGiecj:JW2293.
eco:b2296.
PATRICi32119963. VBIEscCol129921_2391.

Organism-specific databases

EchoBASEiEB0026.
EcoGeneiEG10027. ackA.

Phylogenomic databases

eggNOGiENOG4105C6H. Bacteria.
COG0282. LUCA.
HOGENOMiHOG000288398.
InParanoidiP0A6A3.
KOiK00925.
OMAiHRLKKYI.
PhylomeDBiP0A6A3.

Enzyme and pathway databases

UniPathwayiUPA00340; UER00458.
BioCyciEcoCyc:ACETATEKINA-MONOMER.
ECOL316407:JW2293-MONOMER.
MetaCyc:ACETATEKINA-MONOMER.
SABIO-RKP0A6A3.

Miscellaneous databases

PROiP0A6A3.

Family and domain databases

HAMAPiMF_00020. Acetate_kinase. 1 hit.
InterProiIPR004372. Ac/propionate_kinase.
IPR000890. Aliphatic_acid_kin_short-chain.
IPR023865. Aliphatic_acid_kinase_CS.
[Graphical view]
PANTHERiPTHR21060. PTHR21060. 1 hit.
PfamiPF00871. Acetate_kinase. 1 hit.
[Graphical view]
PIRSFiPIRSF000722. Acetate_prop_kin. 1 hit.
PRINTSiPR00471. ACETATEKNASE.
TIGRFAMsiTIGR00016. ackA. 1 hit.
PROSITEiPS01075. ACETATE_KINASE_1. 1 hit.
PS01076. ACETATE_KINASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACKA_ECOLI
AccessioniPrimary (citable) accession number: P0A6A3
Secondary accession number(s): P15046, P78188, Q59386
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: November 2, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.