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Protein

Probable protein kinase UbiB

Gene

ubiB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Is probably a protein kinase regulator of UbiI activity which is involved in aerobic coenzyme Q (ubiquinone) biosynthesis.UniRule annotation2 Publications

Pathwayi: ubiquinone biosynthesis

This protein regulates the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei153 – 1531ATPUniRule annotation
Active sitei288 – 2881Proton acceptorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi130 – 1389ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • regulation of ubiquinone biosynthetic process Source: UniProtKB-HAMAP
  • ubiquinone biosynthetic process Source: EcoCyc
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:2-OCTAPRENYLPHENOL-HYDROX-MONOMER.
ECOL316407:JW3812-MONOMER.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable protein kinase UbiBUniRule annotation (EC:2.7.-.-UniRule annotation)
Alternative name(s):
Ubiquinone biosynthesis protein UbiBUniRule annotation
Gene namesi
Name:ubiBUniRule annotation
Synonyms:aarF, yigQ, yigR, yigS
Ordered Locus Names:b3835, JW3812
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11476. ubiB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei501 – 52121HelicalUniRule annotationAdd
BLAST
Transmembranei522 – 54221HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene fail to produce coenzyme Q8, and accumulate octaprenylphenol (OPP).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 546546Probable protein kinase UbiBPRO_0000200703Add
BLAST

Proteomic databases

PaxDbiP0A6A0.

Interactioni

Protein-protein interaction databases

BioGridi4260948. 328 interactions.
DIPiDIP-35858N.
IntActiP0A6A0. 12 interactions.
MINTiMINT-1224329.
STRINGi511145.b3835.

Structurei

3D structure databases

ProteinModelPortaliP0A6A0.
SMRiP0A6A0. Positions 54-312.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini124 – 502379Protein kinaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the ABC1 family. UbiB subfamily.UniRule annotation
Contains 1 protein kinase domain.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CNK. Bacteria.
COG0661. LUCA.
HOGENOMiHOG000264440.
InParanoidiP0A6A0.
KOiK03688.
OMAiLIHHAMA.
OrthoDBiEOG61P6SS.
PhylomeDBiP0A6A0.

Family and domain databases

HAMAPiMF_00414. UbiB.
InterProiIPR011009. Kinase-like_dom.
IPR010232. UbiB.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
TIGRFAMsiTIGR01982. UbiB. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A6A0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPGEVRRLY FIIRTFLSYG LDELIPKMRI TLPLRLWRYS LFWMPNRHKD
60 70 80 90 100
KLLGERLRLA LQELGPVWIK FGQMLSTRRD LFPPHIADQL ALLQDKVAPF
110 120 130 140 150
DGKLAKQQIE AAMGGLPVEA WFDDFEIKPL ASASIAQVHT ARLKSNGKEV
160 170 180 190 200
VIKVIRPDIL PVIKADLKLI YRLARWVPRL LPDGRRLRPT EVVREYEKTL
210 220 230 240 250
IDELNLLRES ANAIQLRRNF EDSPMLYIPE VYPDYCSEGM MVMERIYGIP
260 270 280 290 300
VSDVAALEKN GTNMKLLAER GVQVFFTQVF RDSFFHADMH PGNIFVSYEH
310 320 330 340 350
PENPKYIGID CGIVGSLNKE DKRYLAENFI AFFNRDYRKV AELHVDSGWV
360 370 380 390 400
PPDTNVEEFE FAIRTVCEPI FEKPLAEISF GHVLLNLFNT ARRFNMEVQP
410 420 430 440 450
QLVLLQKTLL YVEGVGRQLY PQLDLWKTAK PFLESWIKDQ VGIPALVRAF
460 470 480 490 500
KEKAPFWVEK MPELPELVYD SLRQGKYLQH SVDKIARELQ SNHVRQGQSR
510 520 530 540
YFLGIGATLV LSGTFLLVSR PEWGLMPGWL MAGGLIAWFV GWRKTR
Length:546
Mass (Da):63,203
Last modified:June 7, 2005 - v1
Checksum:iD66C8AD50C05B4EC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti525 – 5251L → V in X05967 (PubMed:3309887).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67630.2. Frameshift.
M87049 Genomic DNA. Translation: AAA67631.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67632.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76838.1.
AP009048 Genomic DNA. Translation: BAE77466.1.
X05967 Genomic DNA. No translation available.
PIRiD65188.
RefSeqiNP_418279.1. NC_000913.3.
WP_000187530.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76838; AAC76838; b3835.
BAE77466; BAE77466; BAE77466.
GeneIDi948322.
KEGGiecj:JW3812.
eco:b3835.
PATRICi32123171. VBIEscCol129921_3951.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M87049 Genomic DNA. Translation: AAA67630.2. Frameshift.
M87049 Genomic DNA. Translation: AAA67631.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67632.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76838.1.
AP009048 Genomic DNA. Translation: BAE77466.1.
X05967 Genomic DNA. No translation available.
PIRiD65188.
RefSeqiNP_418279.1. NC_000913.3.
WP_000187530.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP0A6A0.
SMRiP0A6A0. Positions 54-312.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260948. 328 interactions.
DIPiDIP-35858N.
IntActiP0A6A0. 12 interactions.
MINTiMINT-1224329.
STRINGi511145.b3835.

Proteomic databases

PaxDbiP0A6A0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76838; AAC76838; b3835.
BAE77466; BAE77466; BAE77466.
GeneIDi948322.
KEGGiecj:JW3812.
eco:b3835.
PATRICi32123171. VBIEscCol129921_3951.

Organism-specific databases

EchoBASEiEB1443.
EcoGeneiEG11476. ubiB.

Phylogenomic databases

eggNOGiENOG4105CNK. Bacteria.
COG0661. LUCA.
HOGENOMiHOG000264440.
InParanoidiP0A6A0.
KOiK03688.
OMAiLIHHAMA.
OrthoDBiEOG61P6SS.
PhylomeDBiP0A6A0.

Enzyme and pathway databases

UniPathwayiUPA00232.
BioCyciEcoCyc:2-OCTAPRENYLPHENOL-HYDROX-MONOMER.
ECOL316407:JW3812-MONOMER.

Miscellaneous databases

PROiP0A6A0.

Family and domain databases

HAMAPiMF_00414. UbiB.
InterProiIPR011009. Kinase-like_dom.
IPR010232. UbiB.
IPR004147. UbiB_dom.
[Graphical view]
PfamiPF03109. ABC1. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
TIGRFAMsiTIGR01982. UbiB. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes."
    Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.
    Science 257:771-778(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Random cloning of bent DNA segments from Escherichia coli chromosome and primary characterization of their structures."
    Mizuno T.
    Nucleic Acids Res. 15:6827-6841(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 439-536.
  5. "Identification and characterization of aarF, a locus required for production of ubiquinone in Providencia stuartii and Escherichia coli and for expression of 2'-N-acetyltransferase in P. stuartii."
    Macinga D.R., Cook G.M., Poole R.K., Rather P.N.
    J. Bacteriol. 180:128-135(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN UBIQUINONE BIOSYNTHESIS, DISRUPTION PHENOTYPE.
  6. "Identification of Escherichia coli ubiB, a gene required for the first monooxygenase step in ubiquinone biosynthesis."
    Poon W.W., Davis D.E., Ha H.T., Jonassen T., Rather P.N., Clarke C.F.
    J. Bacteriol. 182:5139-5146(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN UBIQUINONE BIOSYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / RM1734.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  8. Cited for: PROBABLE FUNCTION.
    Strain: K12.

Entry informationi

Entry nameiUBIB_ECOLI
AccessioniPrimary (citable) accession number: P0A6A0
Secondary accession number(s): P27853
, P27854, P27855, P76764, Q2M8E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2005
Last sequence update: June 7, 2005
Last modified: January 20, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.