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Protein

UvrABC system protein A

Gene

uvrA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. UvrA is an ATPase and a DNA-binding protein. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. When the presence of a lesion has been verified by UvrB, the UvrA molecules dissociate.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 38ATP8
Zinc fingeri253 – 280C4-typeUniRule annotationAdd BLAST28
Nucleotide bindingi640 – 647ATP8
Zinc fingeri740 – 766C4-typeUniRule annotationAdd BLAST27

GO - Molecular functioni

  • ATPase activity Source: EcoCyc
  • ATP binding Source: UniProtKB-HAMAP
  • DNA binding Source: EcoCyc
  • excinuclease ABC activity Source: UniProtKB-HAMAP
  • zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  • DNA repair Source: EcoCyc
  • nucleotide-excision repair Source: UniProtKB-HAMAP
  • SOS response Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Excision nuclease

Keywords - Biological processi

DNA damage, DNA excision, DNA repair, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:EG11061-MONOMER.
ECOL316407:JW4019-MONOMER.
MetaCyc:EG11061-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein AUniRule annotation
Short name:
UvrA proteinUniRule annotation
Alternative name(s):
Excinuclease ABC subunit AUniRule annotation
Gene namesi
Name:uvrAUniRule annotation
Synonyms:dinE
Ordered Locus Names:b4058, JW4019
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11061. uvrA.

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • excinuclease repair complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi253C → A, H or S: Reduced activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000930491 – 940UvrABC system protein AAdd BLAST940

Proteomic databases

EPDiP0A698.
PaxDbiP0A698.
PRIDEiP0A698.

Interactioni

Subunit structurei

Forms a heterotetramer with UvrB during the search for lesions (By similarity). Interacts with TRCF (Mfd). UvrB and TRCF binding to UvrA could be mutually exclusive.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
mfdP309582EBI-552091,EBI-554211

Protein-protein interaction databases

BioGridi4262670. 26 interactors.
852853. 1 interactor.
DIPiDIP-35876N.
IntActiP0A698. 14 interactors.
MINTiMINT-1257037.
STRINGi511145.b4058.

Structurei

Secondary structure

1940
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi132 – 138Combined sources7
Beta strandi148 – 160Combined sources13
Helixi163 – 172Combined sources10
Beta strandi176 – 179Combined sources4
Beta strandi182 – 185Combined sources4
Beta strandi196 – 207Combined sources12
Helixi216 – 227Combined sources12
Beta strandi228 – 238Combined sources11
Beta strandi245 – 248Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DFCX-ray2.80B/D131-250[»]
ProteinModelPortaliP0A698.
SMRiP0A698.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini310 – 587ABC transporter 1UniRule annotationAdd BLAST278
Domaini607 – 937ABC transporter 2UniRule annotationAdd BLAST331

Sequence similaritiesi

Belongs to the ABC transporter superfamily. UvrA family.UniRule annotation
Contains 2 ABC transporter domains.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri253 – 280C4-typeUniRule annotationAdd BLAST28
Zinc fingeri740 – 766C4-typeUniRule annotationAdd BLAST27

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiENOG4105C5U. Bacteria.
COG0178. LUCA.
HOGENOMiHOG000050448.
InParanoidiP0A698.
KOiK03701.
OMAiGAIKGWD.
PhylomeDBiP0A698.

Family and domain databases

Gene3Di3.30.1490.20. 2 hits.
3.40.50.300. 4 hits.
HAMAPiMF_00205. UvrA. 1 hit.
InterProiIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013815. ATP_grasp_subdomain_1.
IPR027417. P-loop_NTPase.
IPR004602. UvrA.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00630. uvra. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A698-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKIEVRGAR THNLKNINLV IPRDKLIVVT GLSGSGKSSL AFDTLYAEGQ
60 70 80 90 100
RRYVESLSAY ARQFLSLMEK PDVDHIEGLS PAISIEQKST SHNPRSTVGT
110 120 130 140 150
ITEIHDYLRL LFARVGEPRC PDHDVPLAAQ TVSQMVDNVL SQPEGKRLML
160 170 180 190 200
LAPIIKERKG EHTKTLENLA SQGYIRARID GEVCDLSDPP KLELQKKHTI
210 220 230 240 250
EVVVDRFKVR DDLTQRLAES FETALELSGG TAVVADMDDP KAEELLFSAN
260 270 280 290 300
FACPICGYSM RELEPRLFSF NNPAGACPTC DGLGVQQYFD PDRVIQNPEL
310 320 330 340 350
SLAGGAIRGW DRRNFYYFQM LKSLADHYKF DVEAPWGSLS ANVHKVVLYG
360 370 380 390 400
SGKENIEFKY MNDRGDTSIR RHPFEGVLHN MERRYKETES SAVREELAKF
410 420 430 440 450
ISNRPCASCE GTRLRREARH VYVENTPLPA ISDMSIGHAM EFFNNLKLAG
460 470 480 490 500
QRAKIAEKIL KEIGDRLKFL VNVGLNYLTL SRSAETLSGG EAQRIRLASQ
510 520 530 540 550
IGAGLVGVMY VLDEPSIGLH QRDNERLLGT LIHLRDLGNT VIVVEHDEDA
560 570 580 590 600
IRAADHVIDI GPGAGVHGGE VVAEGPLEAI MAVPESLTGQ YMSGKRKIEV
610 620 630 640 650
PKKRVPANPE KVLKLTGARG NNLKDVTLTL PVGLFTCITG VSGSGKSTLI
660 670 680 690 700
NDTLFPIAQR QLNGATIAEP APYRDIQGLE HFDKVIDIDQ SPIGRTPRSN
710 720 730 740 750
PATYTGVFTP VRELFAGVPE SRARGYTPGR FSFNVRGGRC EACQGDGVIK
760 770 780 790 800
VEMHFLPDIY VPCDQCKGKR YNRETLEIKY KGKTIHEVLD MTIEEAREFF
810 820 830 840 850
DAVPALARKL QTLMDVGLTY IRLGQSATTL SGGEAQRVKL ARELSKRGTG
860 870 880 890 900
QTLYILDEPT TGLHFADIQQ LLDVLHKLRD QGNTIVVIEH NLDVIKTADW
910 920 930 940
IVDLGPEGGS GGGEILVSGT PETVAECEAS HTARFLKPML
Length:940
Mass (Da):103,868
Last modified:April 1, 1988 - v1
Checksum:iD61AAEB6514B860C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13495 Genomic DNA. Translation: AAA24754.1.
U00006 Genomic DNA. Translation: AAC43152.1.
U00096 Genomic DNA. Translation: AAC77028.1.
AP009048 Genomic DNA. Translation: BAE78060.1.
X01621 Genomic DNA. Translation: CAA25764.1.
J01721 Genomic DNA. Translation: AAA24753.1.
PIRiA23869. BVECUA.
RefSeqiNP_418482.1. NC_000913.3.
WP_000357740.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC77028; AAC77028; b4058.
BAE78060; BAE78060; BAE78060.
GeneIDi948559.
KEGGiecj:JW4019.
eco:b4058.
PATRICi32123661. VBIEscCol129921_4179.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M13495 Genomic DNA. Translation: AAA24754.1.
U00006 Genomic DNA. Translation: AAC43152.1.
U00096 Genomic DNA. Translation: AAC77028.1.
AP009048 Genomic DNA. Translation: BAE78060.1.
X01621 Genomic DNA. Translation: CAA25764.1.
J01721 Genomic DNA. Translation: AAA24753.1.
PIRiA23869. BVECUA.
RefSeqiNP_418482.1. NC_000913.3.
WP_000357740.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DFCX-ray2.80B/D131-250[»]
ProteinModelPortaliP0A698.
SMRiP0A698.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262670. 26 interactors.
852853. 1 interactor.
DIPiDIP-35876N.
IntActiP0A698. 14 interactors.
MINTiMINT-1257037.
STRINGi511145.b4058.

Proteomic databases

EPDiP0A698.
PaxDbiP0A698.
PRIDEiP0A698.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC77028; AAC77028; b4058.
BAE78060; BAE78060; BAE78060.
GeneIDi948559.
KEGGiecj:JW4019.
eco:b4058.
PATRICi32123661. VBIEscCol129921_4179.

Organism-specific databases

EchoBASEiEB1054.
EcoGeneiEG11061. uvrA.

Phylogenomic databases

eggNOGiENOG4105C5U. Bacteria.
COG0178. LUCA.
HOGENOMiHOG000050448.
InParanoidiP0A698.
KOiK03701.
OMAiGAIKGWD.
PhylomeDBiP0A698.

Enzyme and pathway databases

BioCyciEcoCyc:EG11061-MONOMER.
ECOL316407:JW4019-MONOMER.
MetaCyc:EG11061-MONOMER.

Miscellaneous databases

PROiP0A698.

Family and domain databases

Gene3Di3.30.1490.20. 2 hits.
3.40.50.300. 4 hits.
HAMAPiMF_00205. UvrA. 1 hit.
InterProiIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR013815. ATP_grasp_subdomain_1.
IPR027417. P-loop_NTPase.
IPR004602. UvrA.
[Graphical view]
PfamiPF00005. ABC_tran. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00630. uvra. 1 hit.
PROSITEiPS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUVRA_ECOLI
AccessioniPrimary (citable) accession number: P0A698
Secondary accession number(s): P07671, P76788, Q2M6P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 1, 1988
Last sequence update: April 1, 1988
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Binds about 2 zinc atoms/molecule.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.