P0A674 (RPOC_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-directed RNA polymerase subunit beta'
Short name=RNAP subunit beta'
EC=2.7.7.6

Alternative name(s):
RNA polymerase subunit beta'
Transcriptase subunit beta'

Gene names

Name:	rpoC
Ordered Locus Names:	Rv0668, MT0696
ORF Names:	MTCI376.07c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	1316 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. HAMAP MF_01322
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). HAMAP MF_01322
Subunit structure	The RNAP catalytic core consists of 2 alpha, 1 beta, 1 beta' and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription By similarity.
Sequence similarities	Belongs to the RNA polymerase beta' chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE
Cellular component	plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1316	1316	DNA-directed RNA polymerase subunit beta' HAMAP MF_01322		PRO_0000067765
Experimental info
	Sequence conflict	594	1	G → E in AAK44922. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A674 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 45BF24839AF53E8B	FASTA	1,316	146,769
	10 20 30 40 50 60 MLDVNFFDEL RIGLATAEDI RQWSYGEVKK PETINYRTLK PEKDGLFCEK IFGPTRDWEC 70 80 90 100 110 120 YCGKYKRVRF KGIICERCGV EVTRAKVRRE RMGHIELAAP VTHIWYFKGV PSRLGYLLDL 130 140 150 160 170 180 APKDLEKIIY FAAYVITSVD EEMRHNELST LEAEMAVERK AVEDQRDGEL EARAQKLEAD 190 200 210 220 230 240 LAELEAEGAK ADARRKVRDG GEREMRQIRD RAQRELDRLE DIWSTFTKLA PKQLIVDENL 250 260 270 280 290 300 YRELVDRYGE YFTGAMGAES IQKLIENFDI DAEAESLRDV IRNGKGQKKL RALKRLKVVA 310 320 330 340 350 360 AFQQSGNSPM GMVLDAVPVI PPELRPMVQL DGGRFATSDL NDLYRRVINR NNRLKRLIDL 370 380 390 400 410 420 GAPEIIVNNE KRMLQESVDA LFDNGRRGRP VTGPGNRPLK SLSDLLKGKQ GRFRQNLLGK 430 440 450 460 470 480 RVDYSGRSVI VVGPQLKLHQ CGLPKLMALE LFKPFVMKRL VDLNHAQNIK SAKRMVERQR 490 500 510 520 530 540 PQVWDVLEEV IAEHPVLLNR APTLHRLGIQ AFEPMLVEGK AIQLHPLVCE AFNADFDGDQ 550 560 570 580 590 600 MAVHLPLSAE AQAEARILML SSNNILSPAS GRPLAMPRLD MVTGLYYLTT EVPGDTGEYQ 610 620 630 640 650 660 PASGDHPETG VYSSPAEAIM AADRGVLSVR AKIKVRLTQL RPPVEIEAEL FGHSGWQPGD 670 680 690 700 710 720 AWMAETTLGR VMFNELLPLG YPFVNKQMHK KVQAAIINDL AERYPMIVVA QTVDKLKDAG 730 740 750 760 770 780 FYWATRSGVT VSMADVLVPP RKKEILDHYE ERADKVEKQF QRGALNHDER NEALVEIWKE 790 800 810 820 830 840 ATDEVGQALR EHYPDDNPII TIVDSGATGN FTQTRTLAGM KGLVTNPKGE FIPRPVKSSF 850 860 870 880 890 900 REGLTVLEYF INTHGARKGL ADTALRTADS GYLTRRLVDV SQDVIVREHD CQTERGIVVE 910 920 930 940 950 960 LAERAPDGTL IRDPYIETSA YARTLGTDAV DEAGNVIVER GQDLGDPEID ALLAAGITQV 970 980 990 1000 1010 1020 KVRSVLTCAT STGVCATCYG RSMATGKLVD IGEAVGIVAA QSIGEPGTQL TMRTFHQGGV 1030 1040 1050 1060 1070 1080 GEDITGGLPR VQELFEARVP RGKAPIADVT GRVRLEDGER FYKITIVPDD GGEEVVYDKI 1090 1100 1110 1120 1130 1140 SKRQRLRVFK HEDGSERVLS DGDHVEVGQQ LMEGSADPHE VLRVQGPREV QIHLVREVQE 1150 1160 1170 1180 1190 1200 VYRAQGVSIH DKHIEVIVRQ MLRRVTIIDS GSTEFLPGSL IDRAEFEAEN RRVVAEGGEP 1210 1220 1230 1240 1250 1260 AAGRPVLMGI TKASLATDSW LSAASFQETT RVLTDAAINC RSDKLNGLKE NVIIGKLIPA 1270 1280 1290 1300 1310 GTGINRYRNI AVQPTEEARA AAYTIPSYED QYYSPDFGAA TGAAVPLDDY GYSDYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"The rpoB gene of Mycobacterium tuberculosis." Miller L.P., Crawford J.T., Shinnick T.M. Antimicrob. Agents Chemother. 38:805-811(1994) [PubMed: 8031050] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-148. Strain: ATCC 25618 / H37Rv.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842574 Genomic DNA. Translation: CAB09389.1. AE000516 Genomic DNA. Translation: AAK44922.1. L27989 Genomic DNA. Translation: AAA21417.1.
PIR	G70535.
RefSeq	NP_215182.1. NC_000962.2. NP_335108.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P0A674.
SMR	P0A674. Positions 9-136.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001410; EBMYCP00000001410; EBMYCG00000001410. EBMYCT00000072799; EBMYCP00000070858; EBMYCG00000072794.
GeneID	888177. 925980.
GenomeReviews	Gene locus MT0696 in contig AE000516_GR. Gene locus Rv0668 in contig AL123456_GR.
KEGG	mtc:MT0696. mtu:Rv0668.
PATRIC	18123251. VBIMycTub22151_0755.
TIGR	MT0696.
Organism-specific databases
TubercuList	Rv0668.
Phylogenomic databases
GeneTree	EBGT00050000016695.
HOGENOM	HBG621785.
OMA	DINDKHI.
PhylomeDB	P0A674.
ProtClustDB	PRK00566.
Family and domain databases
HAMAP	MF_01322. RNApol_bact_RpoC. [Tree]
InterPro	IPR012754. DNA-dir_RpoC_beta_prime. IPR000722. RNA_pol_asu. IPR006592. RNA_pol_N. IPR007080. RNA_pol_Rpb1_1. IPR007066. RNA_pol_Rpb1_3. IPR007083. RNA_pol_Rpb1_4. IPR007081. RNA_pol_Rpb1_5. [Graphical view]
KO	K03046.
Pfam	PF04997. RNA_pol_Rpb1_1. 1 hit. PF00623. RNA_pol_Rpb1_2. 1 hit. PF04983. RNA_pol_Rpb1_3. 1 hit. PF05000. RNA_pol_Rpb1_4. 1 hit. PF04998. RNA_pol_Rpb1_5. 1 hit. [Graphical view]
SMART	SM00663. RPOLA_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR02386. RpoC_TIGR. 1 hit.
ProtoNet	Search...
Other
DrugBank	DB01045. Rifampin. DB01201. Rifapentine.

Entry information

Entry name

RPOC_MYCTU

Accession

Primary (citable) accession number: P0A674
Secondary accession number(s): O06771, P47769

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program