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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

Mb3883

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei100 – 1001SubstrateBy similarity
Metal bindingi101 – 1011Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:Mb3883
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 157157Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolasePRO_0000209620Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A667.
SMRiP0A667. Positions 2-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni78 – 814Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiVYADWNG.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A667-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAISFRPTAD LVDDIGPDVR SCDLQFRQFG GRSQFAGPIS TVRCFQDNAL
60 70 80 90 100
LKSVLSQPSA GGVLVIDGAG SLHTALVGDV IAELARSTGW TGLIVHGAVR
110 120 130 140 150
DAAALRGIDI GIKALGTNPR KSTKTGAGER DVEITLGGVT FVPGDIAYSD

DDGIIVV
Length:157
Mass (Da):16,235
Last modified:March 15, 2005 - v1
Checksum:i92BD93F60B66E793
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO45155.1.
RefSeqiNP_857520.1. NC_002945.3.
WP_003399786.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO45155; CDO45155; Mb3883.
GeneIDi1093859.
KEGGimbo:Mb3883.
PATRICi18010267. VBIMycBov88188_4254.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO45155.1.
RefSeqiNP_857520.1. NC_002945.3.
WP_003399786.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP0A667.
SMRiP0A667. Positions 2-157.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO45155; CDO45155; Mb3883.
GeneIDi1093859.
KEGGimbo:Mb3883.
PATRICi18010267. VBIMycBov88188_4254.

Phylogenomic databases

HOGENOMiHOG000252803.
KOiK02553.
OMAiVYADWNG.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRRAAH_MYCBO
AccessioniPrimary (citable) accession number: P0A667
Secondary accession number(s): P96224, X2BPY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 7, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.