Reviewed, UniProtKB/Swiss-Prot P0A664 (UREG_MYCTU)

Names and origin

Protein names

Recommended name:
    Urease accessory protein ureG

Gene names

Name:	ureG
Ordered Locus Names:	Rv1852, MT1900
ORF Names:	MTCY359.21c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Facilitates the functional incorporation of the urease nickel metallocenter. This process requires GTP hydrolysis, probably effectuated by ureG By similarity.
Subunit structure	Homodimer; disulfide-linked Probable. The physiological role of the disulfide bond has not been proven in vivo. UreD, ureF and ureG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of ureC. The ureE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the ureG family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro GTP binding Inferred from electronic annotation. Source: UniProtKB-KW nickel ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Urease accessory protein ureG HAMAP MF_01389		PRO_0000067669
Regions
	Nucleotide binding	32 – 39	8	GTP By similarity
Amino acid modifications
	Disulfide bond	90		Interchain Potential

Sequences

	Sequence		Length	Mass (Da)
	P0A664-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 702D613945D76BB9	FASTA	224	23,348
	10 20 30 40 50 60 MATHSHPHSH TVPARPRRVR KPGEPLRIGV GGPVGSGKTA LVAALCRQLR GELSLAVLTN 70 80 90 100 110 120 DIYTTEDADF LRTHAVLPDD RIAAVQTGGC PHTAIRDDIT ANLDAIDELM AAHDALDLIL 130 140 150 160 170 180 VESGGDNLTA TFSSGLVDAQ IFVIDVAGGD KVPRKGGPGV TYSDLLVVNK TDLAALVGAD 190 200 210 220 LAVMARDADA VRDGRPTVLQ SLTEDPAASD VVAWVRSQLA ADGV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Purification, characterization, and genetic analysis of Mycobacterium tuberculosis urease, a potentially critical determinant of host-pathogen interaction." Clemens D.L., Lee B.-Y., Horwitz M.A. J. Bacteriol. 177:5644-5652(1995) [PubMed: 7559354] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35801 / TMC 107 / Erdman.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Biochemical studies on Mycobacterium tuberculosis UreG and comparative modeling reveal structural and functional conservation among the bacterial UreG family." Zambelli B., Musiani F., Savini M., Tucker P., Ciurli S. Biochemistry 46:3171-3182(2007) [PubMed: 17309280] [Abstract] Cited for: PROTEIN SEQUENCE OF 171-186 AND 193-224, SUBUNIT, GTPASE ACTIVITY, POSSIBLE DISULFIDE BOND, MODELING.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U33011 Genomic DNA. Translation: AAC43477.1. BX842578 Genomic DNA. Translation: CAB06135.1. AE000516 Genomic DNA. Translation: AAK46171.1.
PIR	D70665.
RefSeq	NP_216368.1. NP_336357.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	885729. 923708.
GenomeReviews	Gene locus MT1900 in contig AE000516_GR. Gene locus Rv1852 in contig AL123456_GR.
KEGG	mtc:MT1900. mtu:Rv1852.
TIGR	MT1900.
Organism-specific databases
TubercuList	Rv1852.
Phylogenomic databases
HOGENOM	P0A664.
OMA	VQIFVID.
Family and domain databases
HAMAP	MF_01389. [Tree]
InterPro	IPR003495. Cbl_biosynth_CobW-like. IPR012202. NiFe_hyd/urase_mat_GTPase. IPR004400. UreG. [Graphical view]
Pfam	PF02492. cobW. 1 hit. [Graphical view]
PIRSF	PIRSF005624. Ni-bind_GTPase. 1 hit.
TIGRFAMs	TIGR00101. ureG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREG_MYCTU

Accession

Primary (citable) accession number: P0A664
Secondary accession number(s): P50051

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)