P0A638 (UBIE_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Demethylmenaquinone methyltransferase
EC=2.1.1.163

Alternative name(s):
Menaquinone biosynthesis methyltransferase ubiE

Gene names

Name:	ubiE
Synonyms:	menH
Ordered Locus Names:	Rv0558, MT0584
ORF Names:	MTCY25D10.37

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	234 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Methyltransferase required for the conversion of demethylmenaquinone (DMKH2) to menaquinone (MKH2) By similarity. HAMAP MF_01813
Catalytic activity	A demethylmenaquinone + S-adenosyl-L-methionine = a menaquinol + S-adenosyl-L-homocysteine. HAMAP MF_01813
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 8/8. HAMAP MF_01813
Miscellaneous	Was identified as a high-confidence drug target. HAMAP MF_01813
Sequence similarities	Belongs to the methyltransferase superfamily. UbiE family.
Sequence caution	The sequence AAK44807.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 234	234	Demethylmenaquinone methyltransferase HAMAP MF_01813		PRO_0000193297

Sequences

	Sequence		Length	Mass (Da)
	P0A638 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 220DE28AD89F2ABD	FASTA	234	25,299
	10 20 30 40 50 60 MSRAALDKDP RDVASMFDGV ARKYDLTNTV LSLGQDRYWR RATRSALRIG PGQKVLDLAA 70 80 90 100 110 120 GTAVSTVELT KSGAWCVAAD FSVGMLAAGA ARKVPKVAGD ATRLPFGDDV FDAVTISFGL 130 140 150 160 170 180 RNVANQQAAL REMARVTRPG GRLLVCEFST PTNALFATAY KEYLMRALPR VARAVSSNPE 190 200 210 220 230 AYEYLAESIR AWPDQAVLAH QISRAGWSGV RWRNLTGGIV ALHAGYKPGK QTPQ

References

[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842573 Genomic DNA. Translation: CAE55299.1. AE000516 Genomic DNA. Translation: AAK44807.1. Different initiation.
PIR	A70549.
RefSeq	NP_334993.2. NC_002755.2. YP_177738.1. NC_000962.2.
3D structure databases
ProteinModelPortal	P0A638.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000001305; EBMYCP00000001305; EBMYCG00000001305. EBMYCT00000071032; EBMYCP00000069091; EBMYCG00000071027.
GeneID	887591. 924958.
GenomeReviews	Gene locus MT0584 in contig AE000516_GR. Gene locus Rv0558 in contig AL123456_GR.
KEGG	mtc:MT0584. mtu:Rv0558.
PATRIC	18122984. VBIMycTub22151_0626.
TIGR	MT0584.
Organism-specific databases
TubercuList	Rv0558.
Phylogenomic databases
GeneTree	EBGT00050000014588.
HOGENOM	HBG559580.
OMA	PYQYLVE.
PhylomeDB	P0A638.
ProtClustDB	PRK00216.
Family and domain databases
HAMAP	MF_01813. UbiE_methyltran. [Tree]
InterPro	IPR004033. UbiE/COQ5_MeTrFase. IPR023576. UbiE/COQ5_MeTrFase_CS. [Graphical view]
KO	K03183.
PANTHER	PTHR10108:SF24. UbiE/COQ5mtfrase. 1 hit.
Pfam	PF01209. Ubie_methyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01934. MenG_MenH_UbiE. 1 hit.
PROSITE	PS01183. UBIE_1. 1 hit. PS01184. UBIE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBIE_MYCTU

Accession

Primary (citable) accession number: P0A638
Secondary accession number(s): O06424

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program