Reviewed, UniProtKB/Swiss-Prot P0A634 (SYC1_MYCTU)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase 1
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 1
      Short name=CysRS 1

Gene names

Name:	cysS1
Synonyms:	cysS
Ordered Locus Names:	Rv3580c, MT3686
ORF Names:	MTCY06G11.27c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Cysteinyl-tRNA synthetase 1 HAMAP MF_00041		PRO_0000159441
Regions
	Motif	35 – 45	11	"HIGH" region HAMAP MF_00041
	Motif	267 – 271	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	33	1	Zinc By similarity
	Metal binding	211	1	Zinc By similarity
	Metal binding	236	1	Zinc By similarity
	Metal binding	240	1	Zinc By similarity
	Binding site	270	1	ATP By similarity
Experimental info
	Sequence conflict	457	1	D → E in AAK48044. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A634-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 515F9D19482ADC0E	FASTA	469	51,855
	10 20 30 40 50 60 MTDRARLRLH DTAAGVVRDF VPLRPGHVSI YLCGATVQGL PHIGHVRSGV AFDILRRWLL 70 80 90 100 110 120 ARGYDVAFIR NVTDIEDKIL AKAAAAGRPW WEWAATHERA FTAAYDALDV LPPSAEPRAT 130 140 150 160 170 180 GHITQMIEMI ERLIQAGHAY TGGGDVYFDV LSYPEYGQLS GHKIDDVHQG EGVAAGKRDQ 190 200 210 220 230 240 RDFTLWKGEK PGEPSWPTPW GRGRPGWHLE CSAMARSYLG PEFDIHCGGM DLVFPHHENE 250 260 270 280 290 300 IAQSRAAGDG FARYWLHNGW VTMGGEKMSK SLGNVLSMPA MLQRVRPAEL RYYLGSAHYR 310 320 330 340 350 360 SMLEFSETAM QDAVKAYVGL EDFLHRVRTR VGAVCPGDPT PRFAEALDDD LSVPIALAEI 370 380 390 400 410 420 HHVRAEGNRA LDAGDHDGAL RSASAIRAMM GILGCDPLDQ RWESRDETSA ALAAVDVLVQ 430 440 450 460 AELQNREKAR EQRNWALADE IRGRLKRAGI EVTDTADGPQ WSLLGGDTK

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842583 Genomic DNA. Translation: CAE55617.1. AE000516 Genomic DNA. Translation: AAK48044.1.
PIR	B70607.
RefSeq	NP_338230.1. YP_177992.1.
3D structure databases
SMR	P0A634. Positions 7-395.
ModBase	Search...
Genome annotation databases
GeneID	888628. 922812.
GenomeReviews	Gene locus MT3686 in contig AE000516_GR.
KEGG	mtc:MT3686. mtu:Rv3580c.
TIGR	MT3686.
Organism-specific databases
TubercuList	Rv3580c.
Phylogenomic databases
HOGENOM	HBG327651.
OMA	DDDIKVY.
Enzyme and pathway databases
BRENDA	6.1.1.16. 809.
Family and domain databases
HAMAP	MF_00041_B. Cys_tRNA_synth_B. [Tree]
InterPro	IPR015804. Cys-tRNA-synt_Ia_C. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC1_MYCTU

Accession

Primary (citable) accession number: P0A634
Secondary accession number(s): P96862

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	February 9, 2010
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)