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P0A621

- TOP1_MYCBO

UniProt

P0A621 - TOP1_MYCBO

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Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Magnesium 1; catalyticUniRule annotation
Sitei48 – 481Interaction with DNAUniRule annotation
Metal bindingi111 – 1111Magnesium 1; catalyticUniRule annotation
Metal bindingi111 – 1111Magnesium 2UniRule annotation
Metal bindingi113 – 1131Magnesium 2UniRule annotation
Sitei167 – 1671Interaction with DNAUniRule annotation
Sitei168 – 1681Interaction with DNAUniRule annotation
Sitei171 – 1711Interaction with DNAUniRule annotation
Sitei176 – 1761Interaction with DNAUniRule annotation
Sitei183 – 1831Interaction with DNAUniRule annotation
Active sitei342 – 3421O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei344 – 3441Interaction with DNAUniRule annotation
Sitei547 – 5471Interaction with DNAUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IUniRule annotation
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topAUniRule annotation
Ordered Locus Names:Mb3670c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934DNA topoisomerase 1PRO_0000145157Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi233413.Mb3670c.

Structurei

3D structure databases

ProteinModelPortaliP0A621.
SMRiP0A621. Positions 17-608.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 142125ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 1966Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1754.
HOGENOMiHOG000004020.
KOiK03168.
OMAiFRSAEYW.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A621-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MADPKTKGRG SGGNGSGRRL VIVESPTKAR KLASYLGSGY IVESSRGHIR
60 70 80 90 100
DLPRAASDVP AKYKSQPWAR LGVNVDADFE PLYIISPEKR STVSELRGLL
110 120 130 140 150
KDVDELYLAT DGDREGEAIA WHLLETLKPR IPVKRMVFHE ITEPAIRAAA
160 170 180 190 200
EHPRDLDIDL VDAQETRRIL DRLYGYEVSP VLWKKVAPKL SAGRVQSVAT
210 220 230 240 250
RIIVARERDR MAFRSAAYWD ILAKLDASVS DPDAAPPTFS ARLTAVAGRR
260 270 280 290 300
VATGRDFDSL GTLRKGDEVI VLDEGSATAL AAGLDGTQLT VASAEEKPYA
310 320 330 340 350
RRPYPPFMTS TLQQEASRKL RFSAERTMSI AQRLYENGYI TYMRTDSTTL
360 370 380 390 400
SESAINAART QARQLYGDEY VAPAPRQYTR KVKNAQEAHE AIRPAGETFA
410 420 430 440 450
TPDAVRRELD GPNIDDFRLY ELIWQRTVAS QMADARGMTL SLRITGMSGH
460 470 480 490 500
QEVVFSATGR TLTFPGFLKA YVETVDELVG GEADDAERRL PHLTPGQRLD
510 520 530 540 550
IVELTPDGHA TNPPARYTEA SLVKALEELG IGRPSTYSSI IKTIQDRGYV
560 570 580 590 600
HKKGSALVPS WVAFAVTGLL EQHFGRLVDY DFTAAMEDEL DEIAAGNERR
610 620 630 640 650
TNWLNNFYFG GDHGVPDSVA RSGGLKKLVG INLEGIDARE VNSIKLFDDT
660 670 680 690 700
HGRPIYVRVG KNGPYLERLV AGDTGEPTPQ RANLSDSITP DELTLQVAEE
710 720 730 740 750
LFATPQQGRT LGLDPETGHE IVAREGRFGP YVTEILPEPA ADAAAAAQGV
760 770 780 790 800
KKRQKAAGPK PRTGSLLRSM DLQTVTLEDA LRLLSLPRVV GVDPASGEEI
810 820 830 840 850
TAQNGRYGPY LKRGNDSRSL VTEDQIFTIT LDEALKIYAE PKRRGRQSAS
860 870 880 890 900
APPLRELGTD PASGKPMVIK DGRFGPYVTD GETNASLRKG DDVASITDER
910 920 930
AAELLADRRA RGPAKRPARK AARKVPAKKA AKRD
Length:934
Mass (Da):102,336
Last modified:March 15, 2005 - v1
Checksum:iB29E2C17897781BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44941.1.
RefSeqiNP_857309.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD95856; CAD95856; Mb3670c.
GeneIDi1093317.
KEGGimbo:Mb3670c.
PATRICi18009784. VBIMycBov88188_4018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44941.1 .
RefSeqi NP_857309.1. NC_002945.3.

3D structure databases

ProteinModelPortali P0A621.
SMRi P0A621. Positions 17-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb3670c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD95856 ; CAD95856 ; Mb3670c .
GeneIDi 1093317.
KEGGi mbo:Mb3670c.
PATRICi 18009784. VBIMycBov88188_4018.

Phylogenomic databases

eggNOGi COG1754.
HOGENOMi HOG000004020.
KOi K03168.
OMAi FRSAEYW.
OrthoDBi EOG6S7XQ9.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiTOP1_MYCBO
AccessioniPrimary (citable) accession number: P0A621
Secondary accession number(s): O08383, Q59567, X2BPY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3