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P0A621

- TOP1_MYCBO

UniProt

P0A621 - TOP1_MYCBO

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Protein
DNA topoisomerase 1
Gene
topA, Mb3670c
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Magnesium. Binds two Mg2+ per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi24 – 241Magnesium 1; catalytic By similarity
Sitei48 – 481Interaction with DNA By similarity
Metal bindingi111 – 1111Magnesium 1; catalytic By similarity
Metal bindingi111 – 1111Magnesium 2 By similarity
Metal bindingi113 – 1131Magnesium 2 By similarity
Sitei167 – 1671Interaction with DNA By similarity
Sitei168 – 1681Interaction with DNA By similarity
Sitei171 – 1711Interaction with DNA By similarity
Sitei176 – 1761Interaction with DNA By similarity
Sitei183 – 1831Interaction with DNA By similarity
Active sitei342 – 3421O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Sitei344 – 3441Interaction with DNA By similarity
Sitei547 – 5471Interaction with DNA By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA binding Source: UniProtKB-KW
  3. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  4. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase I
Omega-protein
Relaxing enzyme
Swivelase
Untwisting enzyme
Gene namesi
Name:topA
Ordered Locus Names:Mb3670c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 934934DNA topoisomerase 1UniRule annotation
PRO_0000145157Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi233413.Mb3670c.

Structurei

3D structure databases

ProteinModelPortaliP0A621.
SMRiP0A621. Positions 17-608.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini18 – 142125Toprim
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni191 – 1966Interaction with DNA By similarity

Sequence similaritiesi

Contains 1 Toprim domain.

Phylogenomic databases

eggNOGiCOG1754.
HOGENOMiHOG000004020.
KOiK03168.
OMAiFRSAEYW.
OrthoDBiEOG6S7XQ9.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00952. Topoisom_1_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01051. topA_bact. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A621-1 [UniParc]FASTAAdd to Basket

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MADPKTKGRG SGGNGSGRRL VIVESPTKAR KLASYLGSGY IVESSRGHIR    50
DLPRAASDVP AKYKSQPWAR LGVNVDADFE PLYIISPEKR STVSELRGLL 100
KDVDELYLAT DGDREGEAIA WHLLETLKPR IPVKRMVFHE ITEPAIRAAA 150
EHPRDLDIDL VDAQETRRIL DRLYGYEVSP VLWKKVAPKL SAGRVQSVAT 200
RIIVARERDR MAFRSAAYWD ILAKLDASVS DPDAAPPTFS ARLTAVAGRR 250
VATGRDFDSL GTLRKGDEVI VLDEGSATAL AAGLDGTQLT VASAEEKPYA 300
RRPYPPFMTS TLQQEASRKL RFSAERTMSI AQRLYENGYI TYMRTDSTTL 350
SESAINAART QARQLYGDEY VAPAPRQYTR KVKNAQEAHE AIRPAGETFA 400
TPDAVRRELD GPNIDDFRLY ELIWQRTVAS QMADARGMTL SLRITGMSGH 450
QEVVFSATGR TLTFPGFLKA YVETVDELVG GEADDAERRL PHLTPGQRLD 500
IVELTPDGHA TNPPARYTEA SLVKALEELG IGRPSTYSSI IKTIQDRGYV 550
HKKGSALVPS WVAFAVTGLL EQHFGRLVDY DFTAAMEDEL DEIAAGNERR 600
TNWLNNFYFG GDHGVPDSVA RSGGLKKLVG INLEGIDARE VNSIKLFDDT 650
HGRPIYVRVG KNGPYLERLV AGDTGEPTPQ RANLSDSITP DELTLQVAEE 700
LFATPQQGRT LGLDPETGHE IVAREGRFGP YVTEILPEPA ADAAAAAQGV 750
KKRQKAAGPK PRTGSLLRSM DLQTVTLEDA LRLLSLPRVV GVDPASGEEI 800
TAQNGRYGPY LKRGNDSRSL VTEDQIFTIT LDEALKIYAE PKRRGRQSAS 850
APPLRELGTD PASGKPMVIK DGRFGPYVTD GETNASLRKG DDVASITDER 900
AAELLADRRA RGPAKRPARK AARKVPAKKA AKRD 934
Length:934
Mass (Da):102,336
Last modified:March 15, 2005 - v1
Checksum:iB29E2C17897781BA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44941.1.
RefSeqiNP_857309.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD95856; CAD95856; Mb3670c.
GeneIDi1093317.
KEGGimbo:Mb3670c.
PATRICi18009784. VBIMycBov88188_4018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44941.1 .
RefSeqi NP_857309.1. NC_002945.3.

3D structure databases

ProteinModelPortali P0A621.
SMRi P0A621. Positions 17-608.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb3670c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD95856 ; CAD95856 ; Mb3670c .
GeneIDi 1093317.
KEGGi mbo:Mb3670c.
PATRICi 18009784. VBIMycBov88188_4018.

Phylogenomic databases

eggNOGi COG1754.
HOGENOMi HOG000004020.
KOi K03168.
OMAi FRSAEYW.
OrthoDBi EOG6S7XQ9.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00952. Topoisom_1_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005733. TopoI_bac-type.
IPR028612. Topoisom_1_IA.
IPR025589. Toprim_C_rpt.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
PF13368. Toprim_C_rpt. 3 hits.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01051. topA_bact. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiTOP1_MYCBO
AccessioniPrimary (citable) accession number: P0A621
Secondary accession number(s): O08383, Q59567, X2BPY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: September 3, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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