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P0A621

- TOP1_MYCBO

UniProt

P0A621 - TOP1_MYCBO

Protein

DNA topoisomerase 1

Gene

topA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

    Cofactori

    Magnesium. Binds two Mg2+ per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi24 – 241Magnesium 1; catalyticUniRule annotation
    Sitei48 – 481Interaction with DNAUniRule annotation
    Metal bindingi111 – 1111Magnesium 1; catalyticUniRule annotation
    Metal bindingi111 – 1111Magnesium 2UniRule annotation
    Metal bindingi113 – 1131Magnesium 2UniRule annotation
    Sitei167 – 1671Interaction with DNAUniRule annotation
    Sitei168 – 1681Interaction with DNAUniRule annotation
    Sitei171 – 1711Interaction with DNAUniRule annotation
    Sitei176 – 1761Interaction with DNAUniRule annotation
    Sitei183 – 1831Interaction with DNAUniRule annotation
    Active sitei342 – 3421O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
    Sitei344 – 3441Interaction with DNAUniRule annotation
    Sitei547 – 5471Interaction with DNAUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. DNA topological change Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding, Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1UniRule annotation (EC:5.99.1.2UniRule annotation)
    Alternative name(s):
    DNA topoisomerase IUniRule annotation
    Omega-protein
    Relaxing enzyme
    Swivelase
    Untwisting enzyme
    Gene namesi
    Name:topAUniRule annotation
    Ordered Locus Names:Mb3670c
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 934934DNA topoisomerase 1PRO_0000145157Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi233413.Mb3670c.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A621.
    SMRiP0A621. Positions 17-608.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini18 – 142125ToprimUniRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni191 – 1966Interaction with DNAUniRule annotation

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.UniRule annotation
    Contains 1 Toprim domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1754.
    HOGENOMiHOG000004020.
    KOiK03168.
    OMAiFRSAEYW.
    OrthoDBiEOG6S7XQ9.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPiMF_00952. Topoisom_1_prok.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 3 hits.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    TIGRFAMsiTIGR01051. topA_bact. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P0A621-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADPKTKGRG SGGNGSGRRL VIVESPTKAR KLASYLGSGY IVESSRGHIR    50
    DLPRAASDVP AKYKSQPWAR LGVNVDADFE PLYIISPEKR STVSELRGLL 100
    KDVDELYLAT DGDREGEAIA WHLLETLKPR IPVKRMVFHE ITEPAIRAAA 150
    EHPRDLDIDL VDAQETRRIL DRLYGYEVSP VLWKKVAPKL SAGRVQSVAT 200
    RIIVARERDR MAFRSAAYWD ILAKLDASVS DPDAAPPTFS ARLTAVAGRR 250
    VATGRDFDSL GTLRKGDEVI VLDEGSATAL AAGLDGTQLT VASAEEKPYA 300
    RRPYPPFMTS TLQQEASRKL RFSAERTMSI AQRLYENGYI TYMRTDSTTL 350
    SESAINAART QARQLYGDEY VAPAPRQYTR KVKNAQEAHE AIRPAGETFA 400
    TPDAVRRELD GPNIDDFRLY ELIWQRTVAS QMADARGMTL SLRITGMSGH 450
    QEVVFSATGR TLTFPGFLKA YVETVDELVG GEADDAERRL PHLTPGQRLD 500
    IVELTPDGHA TNPPARYTEA SLVKALEELG IGRPSTYSSI IKTIQDRGYV 550
    HKKGSALVPS WVAFAVTGLL EQHFGRLVDY DFTAAMEDEL DEIAAGNERR 600
    TNWLNNFYFG GDHGVPDSVA RSGGLKKLVG INLEGIDARE VNSIKLFDDT 650
    HGRPIYVRVG KNGPYLERLV AGDTGEPTPQ RANLSDSITP DELTLQVAEE 700
    LFATPQQGRT LGLDPETGHE IVAREGRFGP YVTEILPEPA ADAAAAAQGV 750
    KKRQKAAGPK PRTGSLLRSM DLQTVTLEDA LRLLSLPRVV GVDPASGEEI 800
    TAQNGRYGPY LKRGNDSRSL VTEDQIFTIT LDEALKIYAE PKRRGRQSAS 850
    APPLRELGTD PASGKPMVIK DGRFGPYVTD GETNASLRKG DDVASITDER 900
    AAELLADRRA RGPAKRPARK AARKVPAKKA AKRD 934
    Length:934
    Mass (Da):102,336
    Last modified:March 15, 2005 - v1
    Checksum:iB29E2C17897781BA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44941.1.
    RefSeqiNP_857309.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD95856; CAD95856; Mb3670c.
    GeneIDi1093317.
    KEGGimbo:Mb3670c.
    PATRICi18009784. VBIMycBov88188_4018.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44941.1 .
    RefSeqi NP_857309.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali P0A621.
    SMRi P0A621. Positions 17-608.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb3670c.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD95856 ; CAD95856 ; Mb3670c .
    GeneIDi 1093317.
    KEGGi mbo:Mb3670c.
    PATRICi 18009784. VBIMycBov88188_4018.

    Phylogenomic databases

    eggNOGi COG1754.
    HOGENOMi HOG000004020.
    KOi K03168.
    OMAi FRSAEYW.
    OrthoDBi EOG6S7XQ9.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    HAMAPi MF_00952. Topoisom_1_prok.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR005733. TopoI_bac-type.
    IPR028612. Topoisom_1_IA.
    IPR025589. Toprim_C_rpt.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    PF13368. Toprim_C_rpt. 3 hits.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    TIGRFAMsi TIGR01051. topA_bact. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiTOP1_MYCBO
    AccessioniPrimary (citable) accession number: P0A621
    Secondary accession number(s): O08383, Q59567, X2BPY1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3