P0A618 (MPT53_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Soluble secreted antigen MPT53

Gene names

Name:	mpt53
Synonyms:	mpb53
Ordered Locus Names:	Rv2878c, MT2946
ORF Names:	MTCY274.09c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	173 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Disulfide oxidoreductase that catalyzes the oxidation of reduced, unfolded secreted proteins to form disulfide bonds. Despite a weak homology to thioredoxin this cannot serve as a substrate for thioredoxin reductase. Ref.4
Subcellular location	Secreted.
Sequence similarities	Belongs to the thioredoxin family.
Sequence caution	The sequence AAK47271.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from direct assay Ref.4. Source: MTBBASE
Cellular component	extracellular region Inferred from direct assay. Source: MTBBASE
Molecular function	oxidoreductase activity Inferred from direct assay Ref.4. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 38

38

Ref.1

Chain

39 – 173

135

Soluble secreted antigen MPT53

PRO_0000034290

Amino acid modifications

Disulfide bond

73 ↔ 76

Redox-active By similarity

Secondary structure

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173

Helix Strand Turn

Details...

Helix

39 – 42

4

Beta strand

45 – 48

4

Beta strand

53 – 55

3

Helix

56 – 59

4

Beta strand

64 – 69

6

Helix

74 – 89

16

Beta strand

93 – 99

7

Helix

104 – 114

11

Beta strand

118 – 122

5

Helix

127 – 131

5

Beta strand

136 – 143

8

Beta strand

149 – 152

4

Beta strand

155 – 157

3

Helix

161 – 170

10

Sequences

	Sequence		Length	Mass (Da)
	P0A618 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: E67C435C368636D9	FASTA	173	18,383
	10 20 30 40 50 60 MSLRLVSPIK AFADGIVAVA IAVVLMFGLA NTPRAVAADE RLQFTATTLS GAPFDGASLQ 70 80 90 100 110 120 GKPAVLWFWT PWCPFCNAEA PSLSQVAAAN PAVTFVGIAT RADVGAMQSF VSKYNLNFTN 130 140 150 160 170 LNDADGVIWA RYNVPWQPAF VFYRADGTST FVNNPTAAMS QDELSGRVAA LTS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression and significance of MPT53 for identification of secreted proteins of Mycobacterium tuberculosis." Wiker H.G., Michell S.L., Hewinson R.G., Spierings E., Nagai S., Harboe M. Microb. Pathog. 26:207-219(1999) [PubMed: 10089161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 39-68.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Gram-positive DsbE proteins function differently from Gram-negative DsbE homologs. A structure to function analysis of DsbE from Mycobacterium tuberculosis." Goulding C.W., Apostol M.I., Gleiter S., Parseghian A., Bardwell J., Gennaro M., Eisenberg D. J. Biol. Chem. 279:3516-3524(2004) [PubMed: 14597624] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.12 ANGSTROMS) OF 38-173, FUNCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y09725 Genomic DNA. Translation: CAA70890.1.
BX842581 Genomic DNA. Translation: CAA98354.1.
AE000516 Genomic DNA. Translation: AAK47271.1. Different initiation.

PIR

A70924.

RefSeq

NP_217394.1. NC_000962.2.
NP_337457.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1LU4	X-ray	1.12	A	38-173	[»]

ProteinModelPortal

P0A618.

SMR

P0A618. Positions 38-171.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000003096; EBMYCP00000003096; EBMYCG00000003094.
EBMYCT00000069970; EBMYCP00000068029; EBMYCG00000069965.

GeneID

887184.
925317.

GenomeReviews

Gene locus MT2946 in contig AE000516_GR.
Gene locus Rv2878c in contig AL123456_GR.

KEGG

mtc:MT2946.
mtu:Rv2878c.

PATRIC

18128250. VBIMycTub22151_3224.

TIGR

MT2946.

Organism-specific databases

TubercuList

Rv2878c.

Phylogenomic databases

GeneTree

EBGT00050000015916.

HOGENOM

HBG565170.

OMA

FVNNPTS.

ProtClustDB

CLSK792139.

Family and domain databases

InterPro

IPR013740. Redoxin.
IPR012336. Thioredoxin-like_fold.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF08534. Redoxin. 1 hit.
[Graphical view]

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

MPT53_MYCTU

Accession

Primary (citable) accession number: P0A618
Secondary accession number(s): Q10804

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program