Sensor-type histidine kinase PrrB - Mycobacterium tuberculosis

Contribute

Send feedback

Read comments (?) or add your own

P0A5Z8 (PRRB_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 74. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sensor-type histidine kinase PrrB
EC=2.7.13.3

Gene names

Name:	prrB
Ordered Locus Names:	Rv0902c, MT0925
ORF Names:	MTCY31.30c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Member of the two-component regulatory system PrrB/PrrA. Involved specifically in early intracellular multiplication of Mycobacterium. Probably phosphorylates PrrA. Ref.3
Catalytic activity	ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Sequence similarities	Contains 1 HAMP domain. Contains 1 histidine kinase domain.
Sequence caution	The sequence AAK45172.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Two-component regulatory system
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	growth involved in symbiotic interaction Inferred from direct assay Ref.3. Source: MTBBASE protein autophosphorylation Inferred from direct assay PubMed 16472747. Source: MTBBASE signal transduction by phosphorylation Inferred from electronic annotation. Source: GOC
Cellular_component	cell wall Inferred from direct assay PubMed 20825248. Source: MTBBASE integral to plasma membrane Inferred from direct assay PubMed 15952732. Source: MTBBASE
Molecular_function	ATP binding Inferred from direct assay PubMed 16472747. Source: MTBBASE phosphorelay sensor kinase activity Inferred from electronic annotation. Source: InterPro protein kinase activity Inferred from direct assay PubMed 16472747. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 446

446

Sensor-type histidine kinase PrrB

PRO_0000074854

Regions

Transmembrane

19 – 39

Helical; Potential

Transmembrane

151 – 171

Helical; Potential

Domain

172 – 222

HAMP

Domain

237 – 446

210

Histidine kinase

Amino acid modifications

Modified residue

240

Phosphohistidine; by autocatalysis By similarity

Secondary structure

446

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P0A5Z8 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: CD84A335CCFFE6D7
Show »

FASTA

446

47,829

        10         20         30         40         50         60 
MNILSRIFAR TPSLRTRVVV ATAIGAAIPV LIVGTVVWVG ITNDRKERLD RRLDEAAGFA 

        70         80         90        100        110        120 
IPFVPRGLDE IPRSPNDQDA LITVRRGNVI KSNSDITLPK LQDDYADTYV RGVRYRVRTV 

       130        140        150        160        170        180 
EIPGPEPTSV AVGATYDATV AETNNLHRRV LLICTFAIGA AAVFAWLLAA FAVRPFKQLA 

       190        200        210        220        230        240 
EQTRSIDAGD EAPRVEVHGA SEAIEIAEAM RGMLQRIWNE QNRTKEALAS ARDFAAVSSH 

       250        260        270        280        290        300 
ELRTPLTAMR TNLEVLSTLD LPDDQRKEVL NDVIRTQSRI EATLSALERL AQGELSTSDD 

       310        320        330        340        350        360 
HVPVDITDLL DRAAHDAARI YPDLDVSLVP SPTCIIVGLP AGLRLAVDNA IANAVKHGGA 

       370        380        390        400        410        420 
TLVQLSAVSS RAGVEIAIDD NGSGVPEGER QVVFERFSRG STASHSGSGL GLALVAQQAQ 

       430        440 
LHGGTASLEN SPLGGARLVL RLPGPS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Transient requirement of the PrrA-PrrB two-component system for early intracellular multiplication of Mycobacterium tuberculosis." Ewann F., Jackson M., Pethe K., Cooper A., Mielcarek N., Ensergueix D., Gicquel B., Locht C., Supply P. Infect. Immun. 70:2256-2263(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION. Strain: Mt103.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842574 Genomic DNA. Translation: CAA97377.1.
AE000516 Genomic DNA. Translation: AAK45172.1. Different initiation.
AL123456 Genomic DNA. Translation: CCP43650.1.

PIR

C70783.

RefSeq

NP_335358.1. NC_002755.2.
YP_006514255.1. NC_018143.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1YS3	X-ray	1.90	A/B/C	297-446	[»]
1YSR	X-ray	1.78	A/B/C	297-446	[»]

ProteinModelPortal

P0A5Z8.

SMR

P0A5Z8. Positions 226-446.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-29034N.

STRING

83332.Rv0902c.

Proteomic databases

PRIDE

P0A5Z8.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAK45172; AAK45172; MT0925.

GeneID

13318806.
926240.

KEGG

mtc:MT0925.
mtu:Rv0902c.
mtv:RVBD_0902c.

PATRIC

18123782. VBIMycTub22151_1015.

Organism-specific databases

TubercuList

Rv0902c.

Phylogenomic databases

eggNOG

COG0642.

HOGENOM

HOG000042110.

K07655.

OMA

TRIFRRT.

ProtClustDB

CLSK790821.

Family and domain databases

Gene3D

1.10.287.130. 1 hit.
3.30.565.10. 1 hit.

InterPro

IPR003660. HAMP_linker_domain.
IPR003594. HATPase_ATP-bd.
IPR004358. Sig_transdc_His_kin-like_C.
IPR003661. Sig_transdc_His_kin_sub1_dim/P.
IPR005467. Sig_transdc_His_kinase_core.
IPR009082. Sig_transdc_His_kinase_dimeric.
[Graphical view]

Pfam

PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]

PRINTS

PR00344. BCTRLSENSOR.

SMART

SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]

SUPFAM

SSF55874. ATP_bd_ATPase. 1 hit.
SSF47384. His_kin_homodim. 1 hit.

PROSITE

PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P0A5Z8.

Entry information

Entry name

PRRB_MYCTU

Accession

Primary (citable) accession number: P0A5Z8
Secondary accession number(s): L0T543, Q10560

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh

Mycobacterium tuberculosis strains ATCC 25618 / H37Rv and CDC 1551 / Oshkosh: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents