ID   INHA_MYCBO              Reviewed;         269 AA.
AC   P0A5Y7; P46533;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   16-JUN-2009, entry version 32.
DE   RecName: Full=Enoyl-[acyl-carrier-protein] reductase [NADH];
DE            EC=1.3.1.9;
DE   AltName: Full=NADH-dependent enoyl-ACP reductase;
GN   Name=inhA; OrderedLocusNames=Mb1520;
OS   Mycobacterium bovis.
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Corynebacterineae; Mycobacteriaceae; Mycobacterium;
OC   Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=1765;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BCG;
RX   MEDLINE=94112548; PubMed=8284673; DOI=10.1126/science.8284673;
RA   Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S.,
RA   Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr.;
RT   "inhA, a gene encoding a target for isoniazid and ethionamide in
RT   Mycobacterium tuberculosis.";
RL   Science 263:227-230(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=WAG201;
RX   MEDLINE=95349387; PubMed=7623658;
RX   DOI=10.1111/j.1365-2958.1995.tb02276.x;
RA   Wilson T.M., de Lisle G.W., Collins D.M.;
RT   "Effect of inhA and katG on isoniazid resistance and virulence of
RT   Mycobacterium bovis.";
RL   Mol. Microbiol. 15:1009-1015(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   MEDLINE=22709107; PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H.,
RA   Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S.,
RA   Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R.,
RA   Parkhill J., Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
CC   -!- FUNCTION: Involved in the resistance against the antituberculosis
CC       drugs isoniazid and ethionamide.
CC   -!- CATALYTIC ACTIVITY: Acyl-[acyl-carrier-protein] + NAD(+) = trans-
CC       2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
CC   -!- PATHWAY: Lipid metabolism; fatty acid biosynthesis.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. FabI subfamily.
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DR   EMBL; U41388; AAB60183.1; -; Genomic_DNA.
DR   EMBL; BX248339; CAD96187.1; -; Genomic_DNA.
DR   RefSeq; NP_855172.1; -.
DR   SMR; P0A5Y7; 2-269.
DR   GeneID; 1092377; -.
DR   GenomeReviews; BX248333_GR; Mb1520.
DR   KEGG; mbo:Mb1520; -.
DR   HOGENOM; P0A5Y7; -.
DR   OMA; P0A5Y7; EAGQQMQ.
DR   BRENDA; 1.3.1.9; 3091.
DR   BindingDB; P0A5Y7; -.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NAD...; IEA:EC.
DR   GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR014358; Enoyl-ACP_Rdtase_NADH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   PANTHER; PTHR19410:SF12; Enoyl-ACP_rdct; 1.
PE   3: Inferred from homology;
KW   Antibiotic resistance; Complete proteome; Fatty acid biosynthesis;
KW   Lipid synthesis; NAD; Oxidoreductase.
FT   CHAIN         1    269       Enoyl-[acyl-carrier-protein] reductase
FT                                [NADH].
FT                                /FTId=PRO_0000054915.
FT   NP_BIND     136    165       NAD (Potential).
FT   BINDING     158    158       Substrate (By similarity).
SQ   SEQUENCE   269 AA;  28528 MW;  F161D6D6A631CA08 CRC64;
     MTGLLDGKRI LVSGIITDSS IAFHIARVAQ EQGAQLVLTG FDRLRLIQRI TDRLPAKAPL
     LELDVQNEEH LASLAGRVTE AIGAGNKLDG VVHSIGFMPQ TGMGINPFFD APYADVSKGI
     HISAYSYASM AKALLPIMNP GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG
     KYGVRSNLVA AGPIRTLAMS AIVGGALGEE AGAQIQLLEE GWDQRAPIGW NMKDATPVAK
     TVCALLSDWL PATTGDIIYA DGGAHTQLL
//