P0A5Y7 (INHA_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Enoyl-[acyl-carrier-protein] reductase [NADH]
EC=1.3.1.9

Alternative name(s):
NADH-dependent enoyl-ACP reductase

Gene names

Name:	inhA
Ordered Locus Names:	Mb1520

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Involved in the resistance against the antituberculosis drugs isoniazid and ethionamide.
Catalytic activity	Acyl-[acyl-carrier-protein] + NAD+ = trans-2,3-dehydroacyl-[acyl-carrier-protein] + NADH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily.

Ontologies

Keywords
Biological process	Antibiotic resistance Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	enoyl-[acyl-carrier-protein] reductase (NADH) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Enoyl-[acyl-carrier-protein] reductase [NADH]		PRO_0000054915
Regions
	Nucleotide binding	136 – 165	30	NAD Potential
Sites
	Binding site	158	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5Y7 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: F161D6D6A631CA08	FASTA	269	28,528
	10 20 30 40 50 60 MTGLLDGKRI LVSGIITDSS IAFHIARVAQ EQGAQLVLTG FDRLRLIQRI TDRLPAKAPL 70 80 90 100 110 120 LELDVQNEEH LASLAGRVTE AIGAGNKLDG VVHSIGFMPQ TGMGINPFFD APYADVSKGI 130 140 150 160 170 180 HISAYSYASM AKALLPIMNP GGSIVGMDFD PSRAMPAYNW MTVAKSALES VNRFVAREAG 190 200 210 220 230 240 KYGVRSNLVA AGPIRTLAMS AIVGGALGEE AGAQIQLLEE GWDQRAPIGW NMKDATPVAK 250 260 TVCALLSDWL PATTGDIIYA DGGAHTQLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"inhA, a gene encoding a target for isoniazid and ethionamide in Mycobacterium tuberculosis." Banerjee A., Dubnau E., Quemard A., Balasubramanian V., Um K.S., Wilson T., Collins D., de Lisle G., Jacobs W.R. Jr. Science 263:227-230(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BCG.
[2]	"Effect of inhA and katG on isoniazid resistance and virulence of Mycobacterium bovis." Wilson T.M., de Lisle G.W., Collins D.M. Mol. Microbiol. 15:1009-1015(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WAG201.
[3]	"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U41388 Genomic DNA. Translation: AAB60183.1. BX248339 Genomic DNA. Translation: CAD96187.1.
RefSeq	NP_855172.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A5Y7.
SMR	P0A5Y7. Positions 2-269.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb1520.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD96187; CAD96187; Mb1520.
GeneID	1092377.
KEGG	mbo:Mb1520.
PATRIC	18005028. VBIMycBov88188_1661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0623.
KO	K11611.
OMA	ISDARAM.
ProtClustDB	PRK07889.
Enzyme and pathway databases
UniPathway	UPA00094.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR014358. Enoyl-ACP_Rdtase_NADH. IPR016040. NAD(P)-bd_dom. [Graphical view]
PIRSF	PIRSF000094. Enoyl-ACP_rdct. 1 hit.
ProtoNet	Search...
Other
BindingDB	P0A5Y7.

Entry information

Entry name

INHA_MYCBO

Accession

Primary (citable) accession number: P0A5Y7
Secondary accession number(s): P46533

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program