Reviewed, UniProtKB/Swiss-Prot P0A5Y4 (FABG_MYCTU)

Names and origin

Protein names

Recommended name:
    3-oxoacyl-[acyl-carrier-protein] reductase
    EC=1.1.1.100
Alternative name(s):
    3-ketoacyl-acyl carrier protein reductase

Gene names

Name:	fabG
Synonyms:	fabG1
Ordered Locus Names:	Rv1483, MT1530
ORF Names:	MTCY277.04

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxoacyl-[acyl-carrier-protein] reductase activity Inferred from electronic annotation. Source: EC NAD or NADH binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 247

247

3-oxoacyl-[acyl-carrier-protein] reductase

PRO_0000054677

Regions

Nucleotide binding

5 – 31

27

NADP By similarity

Sites

Active site

153

1

Proton acceptor By similarity

Binding site

140

1

Substrate By similarity

Secondary structure

1

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247

Helix Strand Turn

Details...

Beta strand

17 – 20

4

Turn

21 – 24

4

Helix

26 – 37

12

Beta strand

41 – 49

9

Beta strand

55 – 59

5

Helix

65 – 79

15

Beta strand

83 – 88

6

Helix

102 – 112

11

Helix

114 – 129

16

Beta strand

133 – 138

6

Helix

151 – 171

21

Helix

172 – 174

3

Beta strand

176 – 183

8

Helix

189 – 193

5

Helix

196 – 202

7

Helix

203 – 205

3

Helix

214 – 225

12

Helix

227 – 229

3

Beta strand

236 – 240

5

Turn

241 – 244

4

Sequences

	Sequence		Length	Mass (Da)
	P0A5Y4-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 70F6254B0FFFCD47	FASTA	247	25,697
	10 20 30 40 50 60 MTATATEGAK PPFVSRSVLV TGGNRGIGLA IAQRLAADGH KVAVTHRGSG APKGLFGVEC 70 80 90 100 110 120 DVTDSDAVDR AFTAVEEHQG PVEVLVSNAG LSADAFLMRM TEEKFEKVIN ANLTGAFRVA 130 140 150 160 170 180 QRASRSMQRN KFGRMIFIGS VSGSWGIGNQ ANYAASKAGV IGMARSIARE LSKANVTANV 190 200 210 220 230 240 VAPGYIDTDM TRALDERIQQ GALQFIPAKR VGTPAEVAGV VSFLASEDAS YISGAVIPVD GGMGMGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Banerjee A., Sugantino M., Sacchettini J.C., Jacobs W.R. Jr. Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U66801 Genomic DNA. Translation: AAC69639.1.
BX842576 Genomic DNA. Translation: CAB02033.1.
AE000516 Genomic DNA. Translation: AAK45795.1.

PIR

F70710.

RefSeq

NP_215999.1.
NP_335981.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1UZL	X-ray	2.00	A/B	1-247	[»]
1UZM	X-ray	1.49	A/B	1-247	[»]
1UZN	X-ray	1.91	A/B	1-247	[»]
2NTN	X-ray	2.30	A/B	1-247	[»]

ModBase

Search...

Genome annotation databases

GeneID

886551.
924439.

GenomeReviews

Gene locus MT1530 in contig AE000516_GR.
Gene locus Rv1483 in contig AL123456_GR.

KEGG

mtc:MT1530.
mtu:Rv1483.

TIGR

MT1530.

Organism-specific databases

TubercuList

Rv1483.

Phylogenomic databases

HOGENOM

P0A5Y4.

OMA

AFLIRMT.

Enzyme and pathway databases

BRENDA

1.1.1.100. 809.

Family and domain databases

InterPro

IPR011284. 3oxo_ACP_reduc.
IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

PANTHER

PTHR19410. ADH_short_C2. 1 hit.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

TIGRFAMs

TIGR01830. 3oxo_ACP_reduc. 1 hit.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

FABG_MYCTU

Accession

Primary (citable) accession number: P0A5Y4
Secondary accession number(s): P71764, Q48930

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 3, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)