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P0A5W9

- RIR1_MYCBO

UniProt

P0A5W9 - RIR1_MYCBO

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Protein
Ribonucleoside-diphosphate reductase subunit alpha
Gene
nrdE, Mb3077c
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides By similarity.

Catalytic activityi

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.

Enzyme regulationi

Under complex allosteric control mediated by deoxynucleoside triphosphates and ATP binding. The type of nucleotide bound at the specificity site determines substrate preference. It seems probable that ATP makes the enzyme reduce CDP and UDP, dGTP favors ADP reduction and dTTP favors GDP reduction By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei172 – 1721Substrate By similarity
Sitei189 – 1891Important for hydrogen atom transfer By similarity
Sitei196 – 1961Allosteric effector binding By similarity
Binding sitei217 – 2171Substrate; via amide nitrogen By similarity
Sitei226 – 2261Allosteric effector binding By similarity
Active sitei397 – 3971Proton acceptor By similarity
Active sitei399 – 3991Cysteine radical intermediate By similarity
Active sitei401 – 4011Proton acceptor By similarity
Sitei426 – 4261Important for hydrogen atom transfer By similarity
Sitei703 – 7031Important for electron transfer By similarity
Sitei704 – 7041Important for electron transfer By similarity
Sitei720 – 7201Interacts with thioredoxin/glutaredoxin By similarity
Sitei723 – 7231Interacts with thioredoxin/glutaredoxin By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

GO - Biological processi

  1. DNA replication Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00326.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonucleoside-diphosphate reductase subunit alpha (EC:1.17.4.1)
Alternative name(s):
Ribonucleotide reductase R1 subunit
Gene namesi
Name:nrdE
Ordered Locus Names:Mb3077c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. ribonucleoside-diphosphate reductase complex Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Ribonucleoside-diphosphate reductase subunit alpha
PRO_0000187219Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi189 ↔ 426Redox-active By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Induced in response to the thiol oxidant diamide.1 Publication

Interactioni

Subunit structurei

Tetramer of two alpha and two beta subunits By similarity.

Protein-protein interaction databases

STRINGi233413.Mb3077c.

Structurei

3D structure databases

ProteinModelPortaliP0A5W9.
SMRiP0A5W9. Positions 26-710.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni188 – 1892Substrate binding By similarity
Regioni397 – 4015Substrate binding By similarity
Regioni599 – 6035Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0209.
HOGENOMiHOG000246165.
KOiK00525.
OrthoDBiEOG6J48HC.

Family and domain databases

InterProiIPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view]
PfamiPF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view]
PRINTSiPR01183. RIBORDTASEM1.
SUPFAMiSSF48168. SSF48168. 1 hit.
TIGRFAMsiTIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEiPS00089. RIBORED_LARGE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A5W9-1 [UniParc]FASTAAdd to Basket

« Hide

MPPTVIAEPV ASGAHASYSG GPGETDYHAL NAMLNLYDAD GKIQFDKDRE    50
AAHQYFLQHV NQNTVFFHNQ DEKLDYLIRE NYYEREVLDQ YSRNFVKTLL 100
DRAYAKKFRF PTFLGAFKYY TSYTLKTFDG KRYLERFEDR VVMVALTLAA 150
GDTALAELLV DEIIDGRFQP ATPTFLNSGK KQRGEPVSCF LLRVEDNMES 200
IGRSINSALQ LSKRGGGVAL LLTNIREHGA PIKNIENQSS GVIPIMKLLE 250
DAFSYANQLG ARQGAGAVYL HAHHPDIYRF LDTKRENADE KIRIKTLSLG 300
VVIPDITFEL AKRNDDMYLF SPYDVERVYG VPFADISVTE KYYEMVDDAR 350
IRKTKIKARE FFQTLAELQF ESGYPYIMFE DTVNRANPID GKITHSNLCS 400
EILQVSTPSL FNEDLSYAKV GKDISCNLGS LNIAKTMDSP DFAQTIEVAI 450
RALTAVSDQT HIKSVPSIEQ GNNDSHAIGL GQMNLHGYLA RERIFYGSDE 500
GIDFTNIYFY TVLYHALRAS NRIAIERGTH FKGFERSKYA SGEFFDKYTD 550
QIWEPKTQKV RQLFADAGIR IPTQDDWRRL KESVQAHGIY NQNLQAVPPT 600
GSISYINHST SSIHPIVSKV EIRKEGKIGR VYYPAPYMTN DNLEYYEDAY 650
EIGYEKIIDT YAAATQHVDQ GLSLTLFFKD TATTRDVNKA QIYAWRKGIK 700
TLYYIRLRQM ALEGTEVEGC VSCML 725
Length:725
Mass (Da):82,441
Last modified:March 15, 2005 - v1
Checksum:i38C315D149331D55
GO

Sequence cautioni

The sequence CDO44346.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44346.1. Different initiation.
RefSeqiNP_856722.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD96764; CAD96764; Mb3077c.
GeneIDi1093421.
KEGGimbo:Mb3077c.
PATRICi18008502. VBIMycBov88188_3381.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44346.1 . Different initiation.
RefSeqi NP_856722.1. NC_002945.3.

3D structure databases

ProteinModelPortali P0A5W9.
SMRi P0A5W9. Positions 26-710.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb3077c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD96764 ; CAD96764 ; Mb3077c .
GeneIDi 1093421.
KEGGi mbo:Mb3077c.
PATRICi 18008502. VBIMycBov88188_3381.

Phylogenomic databases

eggNOGi COG0209.
HOGENOMi HOG000246165.
KOi K00525.
OrthoDBi EOG6J48HC.

Enzyme and pathway databases

UniPathwayi UPA00326 .

Family and domain databases

InterProi IPR013346. NrdE_NrdA.
IPR026459. RNR_1b_NrdE.
IPR000788. RNR_lg_C.
IPR013509. RNR_lsu_N.
IPR013554. RNR_N.
IPR008926. RNR_R1-su_N.
[Graphical view ]
Pfami PF02867. Ribonuc_red_lgC. 1 hit.
PF00317. Ribonuc_red_lgN. 1 hit.
PF08343. RNR_N. 1 hit.
[Graphical view ]
PRINTSi PR01183. RIBORDTASEM1.
SUPFAMi SSF48168. SSF48168. 1 hit.
TIGRFAMsi TIGR02506. NrdE_NrdA. 1 hit.
TIGR04170. RNR_1b_NrdE. 1 hit.
PROSITEi PS00089. RIBORED_LARGE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.
  2. "Thiol specific oxidative stress response in Mycobacteria."
    Dosanjh N.S., Rawat M., Chung J.-H., Av-Gay Y.
    FEMS Microbiol. Lett. 249:87-94(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, INDUCTION.
    Strain: BCG / Pasteur.

Entry informationi

Entry nameiRIR1_MYCBO
AccessioniPrimary (citable) accession number: P0A5W9
Secondary accession number(s): O53296, P50640, X2BN79
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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