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Protein

Protein RecA

Gene

recA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage (By similarity).By similarity
PI-MboI is an endonuclease.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi67 – 74ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Intron homing, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecA
Alternative name(s):
Recombinase A
Cleaved into the following chain:
Alternative name(s):
Mbo RecA intein
Gene namesi
Name:recA
Ordered Locus Names:Mb2756c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000302721 – 251Protein RecA, 1st partAdd BLAST251
ChainiPRO_0000030273252 – 691Endonuclease PI-MboIAdd BLAST440
ChainiPRO_0000030274692 – 790Protein RecA, 2nd partAdd BLAST99

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.By similarity

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiP0A5U5.

Structurei

3D structure databases

ProteinModelPortaliP0A5U5.
SMRiP0A5U5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini366 – 508DOD-type homing endonucleaseAdd BLAST143

Sequence similaritiesi

Belongs to the RecA family.Curated

Phylogenomic databases

KOiK03553.
OMAiFVDFTPL.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 2 hits.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
PR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A5U5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV
60 70 80 90 100
ALGIGGLPRG RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL
110 120 130 140 150
DPDYAKKLGV DTDSLLVSQP DTGEQALEIA DMLIRSGALD IVVIDSVAAL
160 170 180 190 200
VPRAELEGEM GDSHVGLQAR LMSQALRKMT GALNNSGTTA IFINQLRDKI
210 220 230 240 250
GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG NRTRVKVVKN
260 270 280 290 300
KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF
310 320 330 340 350
DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF
360 370 380 390 400
DGFGDSAPIP ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR
410 420 430 440 450
IAATLGCAAH PQGRISLAIA HRPGERNGVA DLCQQAGIYG KLAWEKTIPN
460 470 480 490 500
WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ TGALRVGYTT TSEQLAHQIH
510 520 530 540 550
WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR ISGMDNVTAF
560 570 580 590 600
AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG
610 620 630 640 650
VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM
660 670 680 690 700
LAEELRYSVI REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE
710 720 730 740 750
FDILYGKGIS REGSLIDMGV DQGLIRKSGA WFTYEGEQLG QGKENARNFL
760 770 780 790
VENADVADEI EKKIKEKLGI GAVVTDDPSN DGVLPAPVDF
Length:790
Mass (Da):85,389
Last modified:March 15, 2005 - v1
Checksum:iAD16340D2ED5E572
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44020.1.
RefSeqiNP_856402.1. NC_002945.3.
WP_003414006.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO44020; CDO44020; Mb2756c.
GeneIDi1091758.
KEGGimbo:Mb2756c.
PATRICi18007773. VBIMycBov88188_3019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44020.1.
RefSeqiNP_856402.1. NC_002945.3.
WP_003414006.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP0A5U5.
SMRiP0A5U5.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiN10.009.

Proteomic databases

PRIDEiP0A5U5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO44020; CDO44020; Mb2756c.
GeneIDi1091758.
KEGGimbo:Mb2756c.
PATRICi18007773. VBIMycBov88188_3019.

Phylogenomic databases

KOiK03553.
OMAiFVDFTPL.

Family and domain databases

CDDicd00983. recA. 1 hit.
Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR030934. Intein_C.
IPR004042. Intein_endonuc.
IPR006141. Intein_N.
IPR004860. LAGLIDADG_2.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 2 hits.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF00154. RecA. 1 hit.
[Graphical view]
PRINTSiPR00379. INTEIN.
PR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRECA_MYCBO
AccessioniPrimary (citable) accession number: P0A5U5
Secondary accession number(s): O34519, P26345, X2BLS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.