Protein RecA - Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)

Contribute

Send feedback

Read comments (?) or add your own

P0A5U5 (RECA_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 62. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein RecA

Alternative name(s):
Recombinase A

Cleaved into the following chain:

Endonuclease PI-MboI
EC=3.1.-.-
Alternative name(s):
Mbo RecA intein

Gene names

Name:	recA
Ordered Locus Names:	Mb2756c

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage By similarity. HAMAP-Rule MF_00268 PI-MboI is an endonuclease By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP-Rule MF_00268
Sequence similarities	Belongs to the RecA family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Intron homing SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA-dependent ATPase activity Inferred from electronic annotation. Source: HAMAP damaged DNA binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW single-stranded DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 251

251

Protein RecA, 1st part HAMAP-Rule MF_00268

PRO_0000030272

Chain

252 – 691

440

Endonuclease PI-MboI HAMAP-Rule MF_00268

PRO_0000030273

Chain

692 – 790

Protein RecA, 2nd part HAMAP-Rule MF_00268

PRO_0000030274

Regions

Domain

366 – 508

143

DOD-type homing endonuclease

Nucleotide binding

67 – 74

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P0A5U5 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: AD16340D2ED5E572
Show »

FASTA

790

85,389

        10         20         30         40         50         60 
MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV ALGIGGLPRG 

        70         80         90        100        110        120 
RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL DPDYAKKLGV DTDSLLVSQP 

       130        140        150        160        170        180 
DTGEQALEIA DMLIRSGALD IVVIDSVAAL VPRAELEGEM GDSHVGLQAR LMSQALRKMT 

       190        200        210        220        230        240 
GALNNSGTTA IFINQLRDKI GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG 

       250        260        270        280        290        300 
NRTRVKVVKN KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF 

       310        320        330        340        350        360 
DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF DGFGDSAPIP 

       370        380        390        400        410        420 
ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR IAATLGCAAH PQGRISLAIA 

       430        440        450        460        470        480 
HRPGERNGVA DLCQQAGIYG KLAWEKTIPN WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ 

       490        500        510        520        530        540 
TGALRVGYTT TSEQLAHQIH WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR 

       550        560        570        580        590        600 
ISGMDNVTAF AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG 

       610        620        630        640        650        660 
VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM LAEELRYSVI 

       670        680        690        700        710        720 
REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE FDILYGKGIS REGSLIDMGV 

       730        740        750        760        770        780 
DQGLIRKSGA WFTYEGEQLG QGKENARNFL VENADVADEI EKKIKEKLGI GAVVTDDPSN 

       790 
DGVLPAPVDF

« Hide

References

[1]

"The complete genome sequence of Mycobacterium bovis."
Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.

Hewinson R.G.
Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248343 Genomic DNA. Translation: CAD94941.1.
RefSeq	NP_856402.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A5U5.
SMR	P0A5U5. Positions 1-345, 589-769.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb2756c.
Protein family/group databases
MEROPS	N10.009.
Proteomic databases
PRIDE	P0A5U5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD94941; CAD94941; Mb2756c.
GeneID	1091758.
KEGG	mbo:Mb2756c.
PATRIC	18007773. VBIMycBov88188_3019.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1372.
KO	K03553.
ProtClustDB	PRK09519.
Family and domain databases
Gene3D	3.30.250.10. 1 hit.
HAMAP	MF_00268. RecA.
InterPro	IPR003593. AAA+_ATPase. IPR013765. DNA_recomb/repair_RecA. IPR020584. DNA_recomb/repair_RecA_CS. IPR020588. DNA_recomb_RecA/RadB_ATP-bd. IPR003586. Hint_dom_C. IPR003587. Hint_dom_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. IPR023400. RecA_C. IPR020587. RecA_monomer-monomer_interface. [Graphical view]
PANTHER	PTHR22942:SF1. PTHR22942:SF1. 1 hit.
Pfam	PF00154. RecA. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN. PR00142. RECA.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
SUPFAM	SSF54752. SSF54752. 1 hit.
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR02012. tigrfam_recA. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00321. RECA_1. 1 hit. PS50162. RECA_2. 1 hit. PS50163. RECA_3. 2 hits. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P0A5U5.

Entry information

Entry name

RECA_MYCBO

Accession

Primary (citable) accession number: P0A5U5
Secondary accession number(s): O34519, P26345

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Intein-containing proteins

List of intein-containing protein entries

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following chain:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents