Reviewed, UniProtKB/Swiss-Prot P0A5U5 (RECA_MYCBO)

Names and origin

Protein names

Recommended name:
    Protein recA
Alternative name(s):
    Recombinase A
Cleaved into the following chain:
    1- Recommended name:
            Endonuclease PI-MboI
              EC=3.1.-.-
        Alternative name(s):
            Mbo recA intein

Gene names

Name:	recA
Ordered Locus Names:	Mb2756c

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	790 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation and leading to its autocatalytic cleavage By similarity. PI-MboI is an endonuclease By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity.
Sequence similarities	Belongs to the recA family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair Intron homing SOS response
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Autocatalytic cleavage Protein splicing
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro intron homing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA-dependent ATPase activity Inferred from electronic annotation. Source: HAMAP damaged DNA binding Inferred from electronic annotation. Source: HAMAP endonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW single-stranded DNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Protein recA, 1st part HAMAP MF_00268		PRO_0000030272
	Chain	252 – 691	440	Endonuclease PI-MboI HAMAP MF_00268		PRO_0000030273
	Chain	692 – 790	99	Protein recA, 2nd part HAMAP MF_00268		PRO_0000030274
Regions
	Domain	366 – 508	143	DOD-type homing endonuclease
	Nucleotide binding	67 – 74	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5U5-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: AD16340D2ED5E572	FASTA	790	85,389
	10 20 30 40 50 60 MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV ALGIGGLPRG 70 80 90 100 110 120 RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL DPDYAKKLGV DTDSLLVSQP 130 140 150 160 170 180 DTGEQALEIA DMLIRSGALD IVVIDSVAAL VPRAELEGEM GDSHVGLQAR LMSQALRKMT 190 200 210 220 230 240 GALNNSGTTA IFINQLRDKI GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG 250 260 270 280 290 300 NRTRVKVVKN KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF 310 320 330 340 350 360 DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF DGFGDSAPIP 370 380 390 400 410 420 ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR IAATLGCAAH PQGRISLAIA 430 440 450 460 470 480 HRPGERNGVA DLCQQAGIYG KLAWEKTIPN WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ 490 500 510 520 530 540 TGALRVGYTT TSEQLAHQIH WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR 550 560 570 580 590 600 ISGMDNVTAF AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG 610 620 630 640 650 660 VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM LAEELRYSVI 670 680 690 700 710 720 REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE FDILYGKGIS REGSLIDMGV 730 740 750 760 770 780 DQGLIRKSGA WFTYEGEQLG QGKENARNFL VENADVADEI EKKIKEKLGI GAVVTDDPSN 790 DGVLPAPVDF

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BX248343 Genomic DNA. Translation: CAD94941.1.
RefSeq	NP_856402.1.
3D structure databases
SMR	P0A5U5. Positions 1-254, 253-353.
ModBase	Search...
Genome annotation databases
GeneID	1091758.
GenomeReviews	Gene locus Mb2756c in contig BX248333_GR.
KEGG	mbo:Mb2756c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P0A5U5.
Enzyme and pathway databases
BioCyc	MBOV233413:MB2756C-MON.
Family and domain databases
HAMAP	MF_00268. [Tree]
InterPro	IPR003593. ATPase_AAA+_core. IPR013765. DNA_recomb/repair_RecA. IPR001553. DNA_recomb/repair_RecA/RadB. IPR020584. DNA_recomb/repair_RecA_CS. IPR020588. DNA_recomb_RecA/RadB_ATP-bd. IPR003586. Hedgehog_hint_C. IPR003587. Hedgehog_hint_N. IPR006142. INTEIN. IPR004042. Intein_endonuc. IPR006141. Intein_splicing_site. IPR020587. RecA_monomer-monomer_interface. [Graphical view]
PANTHER	PTHR22942:SF1. RecA. 1 hit.
Pfam	PF00154. RecA. 2 hits. [Graphical view]
PRINTS	PR00379. INTEIN. PR00142. RECA.
SMART	SM00382. AAA. 1 hit. SM00305. HintC. 1 hit. SM00306. HintN. 1 hit. [Graphical view]
TIGRFAMs	TIGR01443. intein_Cterm. 1 hit. TIGR01445. intein_Nterm. 1 hit. TIGR02012. tigrfam_recA. 1 hit.
PROSITE	PS50818. INTEIN_C_TER. 1 hit. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. PS00321. RECA_1. 1 hit. PS50162. RECA_2. 1 hit. PS50163. RECA_3. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RECA_MYCBO

Accession

Primary (citable) accession number: P0A5U5
Secondary accession number(s): O34519, P26345

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	November 24, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)