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P0A5U5

- RECA_MYCBO

UniProt

P0A5U5 - RECA_MYCBO

Protein

Protein RecA

Gene

recA

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 74 (01 Oct 2014)
      Sequence version 1 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage By similarity.By similarity
    PI-MboI is an endonuclease.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi67 – 748ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. DNA-dependent ATPase activity Source: InterPro
    3. endonuclease activity Source: UniProtKB-KW
    4. single-stranded DNA binding Source: InterPro

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. intein-mediated protein splicing Source: InterPro
    3. intron homing Source: UniProtKB-KW
    4. SOS response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    DNA damage, DNA recombination, DNA repair, Intron homing, SOS response

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Protein family/group databases

    MEROPSiN10.009.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein RecA
    Alternative name(s):
    Recombinase A
    Cleaved into the following chain:
    Alternative name(s):
    Mbo RecA intein
    Gene namesi
    Name:recA
    Ordered Locus Names:Mb2756c
    OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
    Taxonomic identifieri233413 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
    ProteomesiUP000001419: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 251251Protein RecA, 1st partPRO_0000030272Add
    BLAST
    Chaini252 – 691440Endonuclease PI-MboIPRO_0000030273Add
    BLAST
    Chaini692 – 79099Protein RecA, 2nd partPRO_0000030274Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.By similarity

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Proteomic databases

    PRIDEiP0A5U5.

    Interactioni

    Protein-protein interaction databases

    STRINGi233413.Mb2756c.

    Structurei

    3D structure databases

    ProteinModelPortaliP0A5U5.
    SMRiP0A5U5. Positions 1-345, 589-769.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini366 – 508143DOD-type homing endonucleaseAdd
    BLAST

    Sequence similaritiesi

    Belongs to the RecA family.Curated

    Phylogenomic databases

    eggNOGiCOG1372.
    KOiK03553.
    OMAiDQGTQDV.
    OrthoDBiEOG6ZKXNZ.

    Family and domain databases

    Gene3Di2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.250.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPiMF_00268. RecA.
    InterProiIPR003593. AAA+_ATPase.
    IPR013765. DNA_recomb/repair_RecA.
    IPR020584. DNA_recomb/repair_RecA_CS.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR023400. RecA_C.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view]
    PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
    PfamiPF14528. LAGLIDADG_3. 1 hit.
    PF00154. RecA. 2 hits.
    [Graphical view]
    PRINTSiPR00379. INTEIN.
    PR00142. RECA.
    SMARTiSM00382. AAA. 1 hit.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF54752. SSF54752. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02012. tigrfam_recA. 1 hit.
    PROSITEiPS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS00321. RECA_1. 1 hit.
    PS50162. RECA_2. 1 hit.
    PS50163. RECA_3. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P0A5U5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV    50
    ALGIGGLPRG RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL 100
    DPDYAKKLGV DTDSLLVSQP DTGEQALEIA DMLIRSGALD IVVIDSVAAL 150
    VPRAELEGEM GDSHVGLQAR LMSQALRKMT GALNNSGTTA IFINQLRDKI 200
    GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG NRTRVKVVKN 250
    KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF 300
    DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF 350
    DGFGDSAPIP ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR 400
    IAATLGCAAH PQGRISLAIA HRPGERNGVA DLCQQAGIYG KLAWEKTIPN 450
    WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ TGALRVGYTT TSEQLAHQIH 500
    WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR ISGMDNVTAF 550
    AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG 600
    VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM 650
    LAEELRYSVI REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE 700
    FDILYGKGIS REGSLIDMGV DQGLIRKSGA WFTYEGEQLG QGKENARNFL 750
    VENADVADEI EKKIKEKLGI GAVVTDDPSN DGVLPAPVDF 790
    Length:790
    Mass (Da):85,389
    Last modified:March 15, 2005 - v1
    Checksum:iAD16340D2ED5E572
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44020.1.
    RefSeqiNP_856402.1. NC_002945.3.

    Genome annotation databases

    EnsemblBacteriaiCAD94941; CAD94941; Mb2756c.
    GeneIDi1091758.
    KEGGimbo:Mb2756c.
    PATRICi18007773. VBIMycBov88188_3019.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX248333 Genomic DNA. Translation: CDO44020.1 .
    RefSeqi NP_856402.1. NC_002945.3.

    3D structure databases

    ProteinModelPortali P0A5U5.
    SMRi P0A5U5. Positions 1-345, 589-769.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 233413.Mb2756c.

    Chemistry

    BindingDBi P0A5U5.

    Protein family/group databases

    MEROPSi N10.009.

    Proteomic databases

    PRIDEi P0A5U5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAD94941 ; CAD94941 ; Mb2756c .
    GeneIDi 1091758.
    KEGGi mbo:Mb2756c.
    PATRICi 18007773. VBIMycBov88188_3019.

    Phylogenomic databases

    eggNOGi COG1372.
    KOi K03553.
    OMAi DQGTQDV.
    OrthoDBi EOG6ZKXNZ.

    Family and domain databases

    Gene3Di 2.170.16.10. 2 hits.
    3.10.28.10. 1 hit.
    3.30.250.10. 1 hit.
    3.40.50.300. 1 hit.
    HAMAPi MF_00268. RecA.
    InterProi IPR003593. AAA+_ATPase.
    IPR013765. DNA_recomb/repair_RecA.
    IPR020584. DNA_recomb/repair_RecA_CS.
    IPR028992. Hedgehog/Intein_dom.
    IPR003586. Hint_dom_C.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR006142. INTEIN.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    IPR027417. P-loop_NTPase.
    IPR020588. RecA_ATP-bd.
    IPR023400. RecA_C.
    IPR020587. RecA_monomer-monomer_interface.
    [Graphical view ]
    PANTHERi PTHR22942:SF1. PTHR22942:SF1. 1 hit.
    Pfami PF14528. LAGLIDADG_3. 1 hit.
    PF00154. RecA. 2 hits.
    [Graphical view ]
    PRINTSi PR00379. INTEIN.
    PR00142. RECA.
    SMARTi SM00382. AAA. 1 hit.
    SM00305. HintC. 1 hit.
    SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF52540. SSF52540. 1 hit.
    SSF54752. SSF54752. 1 hit.
    SSF55608. SSF55608. 1 hit.
    TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
    TIGR01445. intein_Nterm. 1 hit.
    TIGR02012. tigrfam_recA. 1 hit.
    PROSITEi PS50818. INTEIN_C_TER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    PS00321. RECA_1. 1 hit.
    PS50162. RECA_2. 1 hit.
    PS50163. RECA_3. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC BAA-935 / AF2122/97.

    Entry informationi

    Entry nameiRECA_MYCBO
    AccessioniPrimary (citable) accession number: P0A5U5
    Secondary accession number(s): O34519, P26345, X2BLS4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 74 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3