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P0A5U5 (RECA_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein RecA
Alternative name(s):
Recombinase A

Cleaved into the following chain:

  1. Endonuclease PI-MboI
    EC=3.1.-.-
    Alternative name(s):
    Mbo RecA intein
Gene names
Name:recA
Ordered Locus Names:Mb2756c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length790 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage By similarity. HAMAP-Rule MF_00268

PI-MboI is an endonuclease By similarity. HAMAP-Rule MF_00268

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00268.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity. HAMAP-Rule MF_00268

Sequence similarities

Belongs to the RecA family.

Contains 1 DOD-type homing endonuclease domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 251251Protein RecA, 1st part HAMAP-Rule MF_00268
PRO_0000030272
Chain252 – 691440Endonuclease PI-MboI HAMAP-Rule MF_00268
PRO_0000030273
Chain692 – 79099Protein RecA, 2nd part HAMAP-Rule MF_00268
PRO_0000030274

Regions

Domain366 – 508143DOD-type homing endonuclease
Nucleotide binding67 – 748ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A5U5 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: AD16340D2ED5E572

FASTA79085,389
        10         20         30         40         50         60 
MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV ALGIGGLPRG 

        70         80         90        100        110        120 
RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL DPDYAKKLGV DTDSLLVSQP 

       130        140        150        160        170        180 
DTGEQALEIA DMLIRSGALD IVVIDSVAAL VPRAELEGEM GDSHVGLQAR LMSQALRKMT 

       190        200        210        220        230        240 
GALNNSGTTA IFINQLRDKI GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG 

       250        260        270        280        290        300 
NRTRVKVVKN KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF 

       310        320        330        340        350        360 
DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF DGFGDSAPIP 

       370        380        390        400        410        420 
ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR IAATLGCAAH PQGRISLAIA 

       430        440        450        460        470        480 
HRPGERNGVA DLCQQAGIYG KLAWEKTIPN WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ 

       490        500        510        520        530        540 
TGALRVGYTT TSEQLAHQIH WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR 

       550        560        570        580        590        600 
ISGMDNVTAF AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG 

       610        620        630        640        650        660 
VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM LAEELRYSVI 

       670        680        690        700        710        720 
REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE FDILYGKGIS REGSLIDMGV 

       730        740        750        760        770        780 
DQGLIRKSGA WFTYEGEQLG QGKENARNFL VENADVADEI EKKIKEKLGI GAVVTDDPSN 

       790 
DGVLPAPVDF 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248343 Genomic DNA. Translation: CAD94941.1.
RefSeqNP_856402.1. NC_002945.3.

3D structure databases

ProteinModelPortalP0A5U5.
SMRP0A5U5. Positions 1-769.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb2756c.

Chemistry

BindingDBP0A5U5.

Protein family/group databases

MEROPSN10.009.

Proteomic databases

PRIDEP0A5U5.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD94941; CAD94941; Mb2756c.
GeneID1091758.
KEGGmbo:Mb2756c.
PATRIC18007773. VBIMycBov88188_3019.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1372.
KOK03553.
OMADQGTQDV.
OrthoDBEOG6ZKXNZ.
ProtClustDBPRK09519.

Family and domain databases

Gene3D3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPMF_00268. RecA.
InterProIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamPF14528. LAGLIDADG_3. 1 hit.
PF00154. RecA. 2 hits.
[Graphical view]
PRINTSPR00379. INTEIN.
PR00142. RECA.
SMARTSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRECA_MYCBO
AccessionPrimary (citable) accession number: P0A5U5
Secondary accession number(s): O34519, P26345
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: April 16, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Intein-containing proteins

List of intein-containing protein entries