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P0A5U5

- RECA_MYCBO

UniProt

P0A5U5 - RECA_MYCBO

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Protein

Protein RecA

Gene
recA, Mb2756c
Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with LexA causing its activation and leading to its autocatalytic cleavage By similarity.UniRule annotation
PI-MboI is an endonuclease By similarity.UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi67 – 748ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA-dependent ATPase activity Source: InterPro
  3. endonuclease activity Source: UniProtKB-KW
  4. single-stranded DNA binding Source: InterPro

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. intein-mediated protein splicing Source: InterPro
  3. intron homing Source: UniProtKB-KW
  4. SOS response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair, Intron homing, SOS response

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Protein family/group databases

MEROPSiN10.009.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein RecA
Alternative name(s):
Recombinase A
Cleaved into the following chain:
Alternative name(s):
Mbo RecA intein
Gene namesi
Name:recA
Ordered Locus Names:Mb2756c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Protein RecA, 1st partUniRule annotationPRO_0000030272Add
BLAST
Chaini252 – 691440Endonuclease PI-MboIUniRule annotationPRO_0000030273Add
BLAST
Chaini692 – 79099Protein RecA, 2nd partUniRule annotationPRO_0000030274Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation By similarity.UniRule annotation

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Proteomic databases

PRIDEiP0A5U5.

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb2756c.

Structurei

3D structure databases

ProteinModelPortaliP0A5U5.
SMRiP0A5U5. Positions 1-345, 589-769.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini366 – 508143DOD-type homing endonucleaseAdd
BLAST

Sequence similaritiesi

Belongs to the RecA family.

Phylogenomic databases

eggNOGiCOG1372.
KOiK03553.
OMAiDQGTQDV.
OrthoDBiEOG6ZKXNZ.

Family and domain databases

Gene3Di2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00268. RecA.
InterProiIPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view]
PANTHERiPTHR22942:SF1. PTHR22942:SF1. 1 hit.
PfamiPF14528. LAGLIDADG_3. 1 hit.
PF00154. RecA. 2 hits.
[Graphical view]
PRINTSiPR00379. INTEIN.
PR00142. RECA.
SMARTiSM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsiTIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEiPS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P0A5U5-1 [UniParc]FASTAAdd to Basket

« Hide

MTQTPDREKA LELAVAQIEK SYGKGSVMRL GDEARQPISV IPTGSIALDV    50
ALGIGGLPRG RVIEIYGPES SGKTTVALHA VANAQAAGGV AAFIDAEHAL 100
DPDYAKKLGV DTDSLLVSQP DTGEQALEIA DMLIRSGALD IVVIDSVAAL 150
VPRAELEGEM GDSHVGLQAR LMSQALRKMT GALNNSGTTA IFINQLRDKI 200
GVMFGSPETT TGGKALKFYA SVRMDVRRVE TLKDGTNAVG NRTRVKVVKN 250
KCLAEGTRIF DPVTGTTHRI EDVVDGRKPI HVVAAAKDGT LHARPVVSWF 300
DQGTRDVIGL RIAGGAIVWA TPDHKVLTEY GWRAAGELRK GDRVAQPRRF 350
DGFGDSAPIP ADHARLLGYL IGDGRDGWVG GKTPINFINV QRALIDDVTR 400
IAATLGCAAH PQGRISLAIA HRPGERNGVA DLCQQAGIYG KLAWEKTIPN 450
WFFEPDIAAD IVGNLLFGLF ESDGWVSREQ TGALRVGYTT TSEQLAHQIH 500
WLLLRFGVGS TVRDYDPTQK RPSIVNGRRI QSKRQVFEVR ISGMDNVTAF 550
AESVPMWGPR GAALIQAIPE ATQGRRRGSQ ATYLAAEMTD AVLNYLDERG 600
VTAQEAAAMI GVASGDPRGG MKQVLGASRL RRDRVQALAD ALDDKFLHDM 650
LAEELRYSVI REVLPTRRAR TFDLEVEELH TLVAEGVVVH NCSPPFKQAE 700
FDILYGKGIS REGSLIDMGV DQGLIRKSGA WFTYEGEQLG QGKENARNFL 750
VENADVADEI EKKIKEKLGI GAVVTDDPSN DGVLPAPVDF 790
Length:790
Mass (Da):85,389
Last modified:March 15, 2005 - v1
Checksum:iAD16340D2ED5E572
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44020.1.
RefSeqiNP_856402.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD94941; CAD94941; Mb2756c.
GeneIDi1091758.
KEGGimbo:Mb2756c.
PATRICi18007773. VBIMycBov88188_3019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BX248333 Genomic DNA. Translation: CDO44020.1 .
RefSeqi NP_856402.1. NC_002945.3.

3D structure databases

ProteinModelPortali P0A5U5.
SMRi P0A5U5. Positions 1-345, 589-769.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb2756c.

Chemistry

BindingDBi P0A5U5.

Protein family/group databases

MEROPSi N10.009.

Proteomic databases

PRIDEi P0A5U5.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD94941 ; CAD94941 ; Mb2756c .
GeneIDi 1091758.
KEGGi mbo:Mb2756c.
PATRICi 18007773. VBIMycBov88188_3019.

Phylogenomic databases

eggNOGi COG1372.
KOi K03553.
OMAi DQGTQDV.
OrthoDBi EOG6ZKXNZ.

Family and domain databases

Gene3Di 2.170.16.10. 2 hits.
3.10.28.10. 1 hit.
3.30.250.10. 1 hit.
3.40.50.300. 1 hit.
HAMAPi MF_00268. RecA.
InterProi IPR003593. AAA+_ATPase.
IPR013765. DNA_recomb/repair_RecA.
IPR020584. DNA_recomb/repair_RecA_CS.
IPR028992. Hedgehog/Intein_dom.
IPR003586. Hint_dom_C.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR006142. INTEIN.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
IPR027417. P-loop_NTPase.
IPR020588. RecA_ATP-bd.
IPR023400. RecA_C.
IPR020587. RecA_monomer-monomer_interface.
[Graphical view ]
PANTHERi PTHR22942:SF1. PTHR22942:SF1. 1 hit.
Pfami PF14528. LAGLIDADG_3. 1 hit.
PF00154. RecA. 2 hits.
[Graphical view ]
PRINTSi PR00379. INTEIN.
PR00142. RECA.
SMARTi SM00382. AAA. 1 hit.
SM00305. HintC. 1 hit.
SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF52540. SSF52540. 1 hit.
SSF54752. SSF54752. 1 hit.
SSF55608. SSF55608. 1 hit.
TIGRFAMsi TIGR01443. intein_Cterm. 1 hit.
TIGR01445. intein_Nterm. 1 hit.
TIGR02012. tigrfam_recA. 1 hit.
PROSITEi PS50818. INTEIN_C_TER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
PS00321. RECA_1. 1 hit.
PS50162. RECA_2. 1 hit.
PS50163. RECA_3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiRECA_MYCBO
AccessioniPrimary (citable) accession number: P0A5U5
Secondary accession number(s): O34519, P26345, X2BLS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi