P0A5T4 (PUR7_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	purC
Ordered Locus Names:	Rv0780, MT0804
ORF Names:	MTCY369.24

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	growth Inferred from mutant phenotype. Source: MTBBASE purine nucleotide biosynthetic process Inferred from mutant phenotype Ref.1. Source: MTBBASE
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP MF_00137		PRO_0000100843
Experimental info
	Sequence conflict	124	1	A → R in AAB41456. Ref.1
	Sequence conflict	166	1	A → P in AAB41456. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P0A5T4 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 200D1EA37DF98637	FASTA	297	32,930
	10 20 30 40 50 60 MRPALSDYQH VASGKVREIY RVDDEHLLLV ASDRISAYDY VLDSTIPDKG RVLTAMSAFF 70 80 90 100 110 120 FGLVDAPNHL AGPPDDPRIP DEVLGRALVV RRLEMLPVEC VARGYLTGSG LLDYQATGKV 130 140 150 160 170 180 CGIALPPGLV EASRFATPLF TPATKAALGD HDENISFDRV VEMVGALRAN QLRDRTLQTY 190 200 210 220 230 240 VQAADHALTR GIIIADTKFE FGIDRHGNLL LADEIFTPDS SRYWPADDYR AGVVQTSFDK 250 260 270 280 290 QFVRSWLTGS ESGWDRGSDR PPPPLPEHIV EATRARYINA YERISELKFD DWIGPGA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Mycobacterium tuberculosis purine biosynthetic pathway: isolation and characterization of the purC and purL genes." Jackson M., Berthet F.-X., Otal I., Rauzier J., Martin C., Gicquel B., Guilhot C. Microbiology 142:2439-2447(1996) [PubMed: 8828210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U34957 Genomic DNA. Translation: AAB41456.1. BX842574 Genomic DNA. Translation: CAB02370.1. AE000516 Genomic DNA. Translation: AAK45046.1.
PIR	G70708.
RefSeq	NP_215294.1. NC_000962.2. NP_335232.1. NC_002755.2.
3D structure databases
ProteinModelPortal	P0A5T4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBMYCT00000000922; EBMYCP00000000922; EBMYCG00000000922. EBMYCT00000069768; EBMYCP00000067827; EBMYCG00000069763.
GeneID	888928. 926107.
GenomeReviews	Gene locus MT0804 in contig AE000516_GR. Gene locus Rv0780 in contig AL123456_GR.
KEGG	mtc:MT0804. mtu:Rv0780.
PATRIC	18123508. VBIMycTub22151_0882.
TIGR	MT0804.
Organism-specific databases
TubercuList	Rv0780.
Phylogenomic databases
GeneTree	EBGT00050000017539.
HOGENOM	HBG555040.
OMA	LTLMDEV.
PhylomeDB	P0A5T4.
ProtClustDB	PRK13961.
Family and domain databases
HAMAP	MF_00137. SAICAR_synth. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synth. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
KO	K01923.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
TIGRFAMs	TIGR00081. PurC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR7_MYCTU

Accession

Primary (citable) accession number: P0A5T4
Secondary accession number(s): P77904, Q59566

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program