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P0A5T1

- HPRT_MYCBO

UniProt

P0A5T1 - HPRT_MYCBO

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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

hpt

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei126 – 1261Proton acceptorBy similarity
Binding sitei154 – 1541IMPBy similarity
Binding sitei176 – 1761IMP; via carbonyl oxygenBy similarity
Metal bindingi182 – 1821MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi122 – 13110IMPBy similarity
Nucleotide bindingi181 – 1822IMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB-EC
  2. hypoxanthine phosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. IMP salvage Source: UniProtKB-UniPathway
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:hpt
Synonyms:hprT
Ordered Locus Names:Mb3648c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139610Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi233413.Mb3648c.

Structurei

3D structure databases

ProteinModelPortaliP0A5T1.
SMRiP0A5T1. Positions 22-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
HOGENOMiHOG000236520.
KOiK00760.
OrthoDBiEOG693GNP.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A5T1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHVTQSSSAI TPGQTAELYP GDIKSVLLTA EQIQARIAEL GEQIGNDYRE
60 70 80 90 100
LSATTGQDLL LITVLKGAVL FVTDLARAIP VPTQFEFMAV SSYGSSTSSS
110 120 130 140 150
GVVRILKDLD RDIHGRDVLI VEDVVDSGLT LSWLSRNLTS RNPRSLRVCT
160 170 180 190 200
LLRKPDAVHA NVEIAYVGFD IPNDFVVGYG LDYDERYRDL SYIGTLDPRV

YQ
Length:202
Mass (Da):22,251
Last modified:March 15, 2005 - v1
Checksum:i17AD6817A2F8ED90
GO

Sequence cautioni

The sequence CDO44919.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44919.1. Different initiation.
RefSeqiNP_857287.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCAD95834; CAD95834; Mb3648c.
GeneIDi1093331.
KEGGimbo:Mb3648c.
PATRICi18009731. VBIMycBov88188_3993.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO44919.1 . Different initiation.
RefSeqi NP_857287.1. NC_002945.3.

3D structure databases

ProteinModelPortali P0A5T1.
SMRi P0A5T1. Positions 22-202.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 233413.Mb3648c.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAD95834 ; CAD95834 ; Mb3648c .
GeneIDi 1093331.
KEGGi mbo:Mb3648c.
PATRICi 18009731. VBIMycBov88188_3993.

Phylogenomic databases

eggNOGi COG0634.
HOGENOMi HOG000236520.
KOi K00760.
OrthoDBi EOG693GNP.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiHPRT_MYCBO
AccessioniPrimary (citable) accession number: P0A5T1
Secondary accession number(s): O06383, P96906, X2BNW2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 26, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3