Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P0A5T1 (HPRT_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 52. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Hypoxanthine-guanine phosphoribosyltransferase

Short name=HGPRT
Short name=HGPRTase
EC=2.4.2.8
Gene names
Name:hpt
Synonyms:hprT
Ordered Locus Names:Mb3648c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.

GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactor

Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.

Pathway

Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the purine/pyrimidine phosphoribosyltransferase family.

Sequence caution

The sequence CDO44919.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
   Biological processPurine salvage
   Cellular componentCytoplasm
   LigandMagnesium
Metal-binding
Nucleotide-binding
   Molecular functionGlycosyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processIMP salvage

Inferred from electronic annotation. Source: UniProtKB-UniPathway

purine ribonucleoside salvage

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionguanine phosphoribosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

hypoxanthine phosphoribosyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

nucleotide binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 202202Hypoxanthine-guanine phosphoribosyltransferase
PRO_0000139610

Regions

Nucleotide binding122 – 13110IMP By similarity
Nucleotide binding181 – 1822IMP By similarity

Sites

Active site1261Proton acceptor By similarity
Metal binding1821Magnesium By similarity
Binding site1541IMP By similarity
Binding site1761IMP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A5T1 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 17AD6817A2F8ED90

FASTA20222,251
        10         20         30         40         50         60 
MHVTQSSSAI TPGQTAELYP GDIKSVLLTA EQIQARIAEL GEQIGNDYRE LSATTGQDLL 

        70         80         90        100        110        120 
LITVLKGAVL FVTDLARAIP VPTQFEFMAV SSYGSSTSSS GVVRILKDLD RDIHGRDVLI 

       130        140        150        160        170        180 
VEDVVDSGLT LSWLSRNLTS RNPRSLRVCT LLRKPDAVHA NVEIAYVGFD IPNDFVVGYG 

       190        200 
LDYDERYRDL SYIGTLDPRV YQ 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BX248333 Genomic DNA. Translation: CDO44919.1. Different initiation.
RefSeqNP_857287.1. NC_002945.3.

3D structure databases

ProteinModelPortalP0A5T1.
SMRP0A5T1. Positions 22-201.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING233413.Mb3648c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD95834; CAD95834; Mb3648c.
GeneID1093331.
KEGGmbo:Mb3648c.
PATRIC18009731. VBIMycBov88188_3993.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0634.
HOGENOMHOG000236520.
KOK00760.
OrthoDBEOG693GNP.

Enzyme and pathway databases

UniPathwayUPA00591; UER00648.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
InterProIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMSSF53271. SSF53271. 1 hit.
TIGRFAMsTIGR01203. HGPRTase. 1 hit.
PROSITEPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHPRT_MYCBO
AccessionPrimary (citable) accession number: P0A5T1
Secondary accession number(s): O06383, P96906, X2BNW2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 11, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways