P0A5S8 (Y2782_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uncharacterized zinc protease Rv2782c/MT2852
EC=3.4.24.-

Gene names

Ordered Locus Names:	Rv2782c, MT2852
ORF Names:	MTV002.47c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	438 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Cofactor	Binds 1 zinc ion per subunit By similarity.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	plasma membrane Inferred from direct assay PubMed 14532352. Source: MTBBASE
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 438	438	Uncharacterized zinc protease Rv2782c/MT2852		PRO_0000074423
Sites
	Active site	62	1	Proton acceptor By similarity
	Metal binding	59	1	Zinc By similarity
	Metal binding	63	1	Zinc By similarity
	Metal binding	139	1	Zinc By similarity
Experimental info
	Sequence conflict	196	1	R → W in AAK47171. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P0A5S8 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: C16707238706DE71	FASTA	438	47,072
	10 20 30 40 50 60 MPRRSPADPA AALAPRRTTL PGGLRVVTEF LPAVHSASVG VWVGVGSRDE GATVAGAAHF 70 80 90 100 110 120 LEHLLFKSTP TRSAVDIAQA MDAVGGELNA FTAKEHTCYY AHVLGSDLPL AVDLVADVVL 130 140 150 160 170 180 NGRCAADDVE VERDVVLEEI AMRDDDPEDA LADMFLAALF GDHPVGRPVI GSAQSVSVMT 190 200 210 220 230 240 RAQLQSFHLR RYTPERMVVA AAGNVDHDGL VALVREHFGS RLVRGRRPVA PRKGTGRVNG 250 260 270 280 290 300 SPRLTLVSRD AEQTHVSLGI RTPGRGWEHR WALSVLHTAL GGGLSSRLFQ EVRETRGLAY 310 320 330 340 350 360 SVYSALDLFA DSGALSVYAA CLPERFADVM RVTADVLESV ARDGITEAEC GIAKGSLRGG 370 380 390 400 410 420 LVLGLEDSSS RMSRLGRSEL NYGKHRSIEH TLRQIEQVTV EEVNAVARHL LSRRYGAAVL 430 GPHGSKRSLP QQLRAMVG

References

[1]

"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.

[2]

"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842580 Genomic DNA. Translation: CAA15577.1. AE000516 Genomic DNA. Translation: AAK47171.1. AL123456 Genomic DNA. Translation: CCP45581.1.
PIR	E70883.
RefSeq	NP_217298.1. NC_000962.3. NP_337357.1. NC_002755.2. YP_006516228.1. NC_018143.1.
3D structure databases
ProteinModelPortal	P0A5S8.
SMR	P0A5S8. Positions 19-433.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv2782c.
Proteomic databases
PRIDE	P0A5S8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK47171; AAK47171; MT2852.
GeneID	13317567. 888470. 925431.
KEGG	mtc:MT2852. mtu:Rv2782c. mtv:RVBD_2782c.
PATRIC	18128026. VBIMycTub22151_3112.
Organism-specific databases
TubercuList	Rv2782c.
Phylogenomic databases
eggNOG	COG0612.
HOGENOM	HOG000242449.
KO	K01417. K01422.
OMA	CTSVTEN.
ProtClustDB	CLSK792074.
Family and domain databases
Gene3D	3.30.830.10. 2 hits.
InterPro	IPR011249. Metalloenz_LuxS/M16. IPR011237. Pept_M16_dom. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR007863. Peptidase_M16_C. [Graphical view]
Pfam	PF00675. Peptidase_M16. 1 hit. PF05193. Peptidase_M16_C. 1 hit. [Graphical view]
SUPFAM	SSF63411. Metalloenz_metal-bd. 2 hits.
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y2782_MYCTU

Accession

Primary (citable) accession number: P0A5S8
Secondary accession number(s): L0TAV3, O33324

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program