P0A5S7 (PPNK_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23

Gene names

Name:	ppnK
Ordered Locus Names:	Mb1721

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus By similarity. HAMAP-Rule MF_00361
Catalytic activity	ATP + NAD+ = ADP + NADP+. HAMAP-Rule MF_00361
Cofactor	Divalent metal ions By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NAD kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding NAD NADP Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	NAD metabolic process Inferred from electronic annotation. Source: InterPro NADP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ kinase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Inorganic polyphosphate/ATP-NAD kinase HAMAP-Rule MF_00361		PRO_0000120636

Sequences

	Sequence		Length	Mass (Da)
	P0A5S7 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 68817BE570B6645B	FASTA	307	32,904
	10 20 30 40 50 60 MTAHRSVLLV VHTGRDEATE TARRVEKVLG DNKIALRVLS AEAVDRGSLH LAPDDMRAMG 70 80 90 100 110 120 VEIEVVDADQ HAADGCELVL VLGGDGTFLR AAELARNASI PVLGVNLGRI GFLAEAEAEA 130 140 150 160 170 180 IDAVLEHVVA QDYRVEDRLT LDVVVRQGGR IVNRGWALNE VSLEKGPRLG VLGVVVEIDG 190 200 210 220 230 240 RPVSAFGCDG VLVSTPTGST AYAFSAGGPV LWPDLEAILV VPNNAHALFG RPMVTSPEAT 250 260 270 280 290 300 IAIEIEADGH DALVFCDGRR EMLIPAGSRL EVTRCVTSVK WARLDSAPFT DRLVRKFRLP VTGWRGK

References

[1]

"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX248339 Genomic DNA. Translation: CAD96389.1.
RefSeq	NP_855374.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A5S7.
SMR	P0A5S7. Positions 2-305.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb1721.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD96389; CAD96389; Mb1721.
GeneID	1092671.
KEGG	mbo:Mb1721.
PATRIC	18005461. VBIMycBov88188_1877.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0061.
HOGENOM	HOG000227223.
KO	K00858.
OMA	GVLWCDG.
ProtClustDB	PRK03372.
Family and domain databases
Gene3D	2.60.200.30. 1 hit. 3.40.50.10330. 1 hit.
HAMAP	MF_00361. NAD_kinase.
InterPro	IPR017438. ATP-NAD_kinase_dom_1. IPR016064. ATP-NAD_kinase_PpnK-typ. IPR017437. ATP-NAD_kinase_PpnK-typ_all-b. IPR002504. PolyP/ATP_NADK_prd. [Graphical view]
PANTHER	PTHR20275. PTHR20275. 1 hit.
Pfam	PF01513. NAD_kinase. 1 hit. [Graphical view]
SUPFAM	SSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNet	Search...
Other
BindingDB	P0A5S7.

Entry information

Entry name

PPNK_MYCBO

Accession

Primary (citable) accession number: P0A5S7
Secondary accession number(s): O33196

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program