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P0A5S6 (PPNK_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Inorganic polyphosphate/ATP-NAD kinase
Short name=Poly(P)/ATP NAD kinase
EC=2.7.1.23
Gene names
Name:ppnK
Ordered Locus Names:Rv1695, MT1734
ORF Names:MTCI125.17
OrganismMycobacterium tuberculosis
Taxonomic identifier1773 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length307 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus. HAMAP MF_00361

Catalytic activity

ATP + NAD+ = ADP + NADP+. HAMAP MF_00361

Cofactor

Divalent metal ions.

Subunit structure

Homotetramer.

Subcellular location

Cytoplasm HAMAP MF_00361.

Miscellaneous

Was identified as a high-confidence drug target. HAMAP MF_00361

Sequence similarities

Belongs to the NAD kinase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 307307Inorganic polyphosphate/ATP-NAD kinase HAMAP MF_00361
PRO_0000120635

Secondary structure

1 ................................................... 307
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P0A5S6 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 68817BE570B6645B

FASTA30732,904
        10         20         30         40         50         60 
MTAHRSVLLV VHTGRDEATE TARRVEKVLG DNKIALRVLS AEAVDRGSLH LAPDDMRAMG 

        70         80         90        100        110        120 
VEIEVVDADQ HAADGCELVL VLGGDGTFLR AAELARNASI PVLGVNLGRI GFLAEAEAEA 

       130        140        150        160        170        180 
IDAVLEHVVA QDYRVEDRLT LDVVVRQGGR IVNRGWALNE VSLEKGPRLG VLGVVVEIDG 

       190        200        210        220        230        240 
RPVSAFGCDG VLVSTPTGST AYAFSAGGPV LWPDLEAILV VPNNAHALFG RPMVTSPEAT 

       250        260        270        280        290        300 
IAIEIEADGH DALVFCDGRR EMLIPAGSRL EVTRCVTSVK WARLDSAPFT DRLVRKFRLP 


VTGWRGK

« Hide

References

« Hide 'large scale' references
[1]"Inorganic polyphosphate / ATP-NAD kinase of Micrococcus flavus and Mycobacterium tuberculosis H37Rv."
Kawai S., Mori S., Mukai T., Suzuki S., Yamada T., Hashimoto W., Murata K.
Biochem. Biophys. Res. Commun. 276:57-63(2000) [PubMed: 11006082] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
Strain: ATCC 25618 / H37Rv.
[2]"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence."
Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. expand/collapse author list , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G.
Nature 393:537-544(1998) [PubMed: 9634230] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 25618 / H37Rv.
[3]"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains."
Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. expand/collapse author list , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.
J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CDC 1551 / Oshkosh.
[4]"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis."
Raman K., Yeturu K., Chandra N.
BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract]
Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AB044336 Genomic DNA. Translation: BAB21478.1.
BX842577 Genomic DNA. Translation: CAB10952.1.
AE000516 Genomic DNA. Translation: AAK46003.1.
PIRF70502.
RefSeqNP_216211.1. NC_000962.2.
NP_336189.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U0RX-ray2.80A/B/C/D1-307[»]
1U0TX-ray2.30A/B1-307[»]
1Y3HX-ray2.80A/B1-307[»]
1Y3IX-ray2.60A/B1-307[»]
ProteinModelPortalP0A5S6.
SMRP0A5S6. Positions 2-305.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBMYCT00000002303; EBMYCP00000002303; EBMYCG00000002301.
EBMYCT00000069036; EBMYCP00000067095; EBMYCG00000069031.
GeneID885660.
923972.
GenomeReviewsGene locus MT1734 in contig AE000516_GR.
Gene locus Rv1695 in contig AL123456_GR.
KEGGmtc:MT1734.
mtu:Rv1695.
PATRIC18125566. VBIMycTub22151_1897.
TIGRMT1734.

Organism-specific databases

TubercuListRv1695.

Phylogenomic databases

GeneTreeEBGT00050000016530.
HOGENOMHBG713904.
OMAGPVVWPE.
PhylomeDBP0A5S6.
ProtClustDBPRK03372.

Enzyme and pathway databases

BRENDA2.7.1.23. 3445.

Family and domain databases

HAMAPMF_00361. NAD_kinase.
[Tree]
InterProIPR016064. ATP-NAD_kinase_PpnK-typ.
IPR017438. ATP-NAD_kinase_PpnK-typ_a/b.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR002504. polyP/ATP_NADK_prd.
[Graphical view]
Gene3DG3DSA:2.60.200.30. ATP-NAD_kinase_PpnK-typ. 1 hit.
G3DSA:3.40.50.10330. ATP-NAD_kinase_PpnK-typ_a/b. 1 hit.
KOK00858.
PANTHERPTHR20275. ATP_NADK. 1 hit.
PfamPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMSSF111331. ATP-NAD_kinase_PpnK-typ. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPNK_MYCTU
AccessionPrimary (citable) accession number: P0A5S6
Secondary accession number(s): O33196
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents