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Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Key component of a signal transduction pathway that regulates cell growth and cell division via phosphorylation of target proteins.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei40 – 401ATPPROSITE-ProRule annotation
Active sitei138 – 1381Proton acceptorPROSITE-ProRule annotation
Metal bindingi143 – 1431MagnesiumBy similarity
Metal bindingi156 – 1561MagnesiumBy similarity
Binding sitei156 – 1561ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 259ATPPROSITE-ProRule annotation
Nucleotide bindingi93 – 953ATPPROSITE-ProRule annotation
Nucleotide bindingi140 – 1434ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknB (EC:2.7.11.1)
Gene namesi
Name:pknB
Ordered Locus Names:Mb0014c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 332332CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei333 – 35321HelicalSequence AnalysisAdd
BLAST
Topological domaini354 – 626273ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 626626Serine/threonine-protein kinase PknBPRO_0000171206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei166 – 1661Phosphoserine; by autocatalysisBy similarity
Modified residuei169 – 1691Phosphoserine; by autocatalysisBy similarity
Modified residuei171 – 1711Phosphothreonine; by autocatalysisBy similarity
Modified residuei173 – 1731Phosphothreonine; by autocatalysisBy similarity
Modified residuei294 – 2941Phosphothreonine; by autocatalysisBy similarity
Modified residuei295 – 2951Phosphoserine; by autocatalysisBy similarity
Modified residuei309 – 3091Phosphothreonine; by autocatalysisBy similarity

Post-translational modificationi

Autophosphorylated. Dephosphorylated by PstP (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A5S5.
SMRiP0A5S5. Positions 2-299, 355-626.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 274264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini356 – 42267PASTA 1PROSITE-ProRule annotationAdd
BLAST
Domaini423 – 49068PASTA 2PROSITE-ProRule annotationAdd
BLAST
Domaini491 – 55767PASTA 3PROSITE-ProRule annotationAdd
BLAST
Domaini558 – 62669PASTA 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 4 PASTA domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000037185.
KOiK08884.
OMAiPANIMIN.
OrthoDBiEOG6B35XT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A5S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPSHLSDR YELGEILGFG GMSEVHLARD LRLHRDVAVK VLRADLARDP
60 70 80 90 100
SFYLRFRREA QNAAALNHPA IVAVYDTGEA ETPAGPLPYI VMEYVDGVTL
110 120 130 140 150
RDIVHTEGPM TPKRAIEVIA DACQALNFSH QNGIIHRDVK PANIMISATN
160 170 180 190 200
AVKVMDFGIA RAIADSGNSV TQTAAVIGTA QYLSPEQARG DSVDARSDVY
210 220 230 240 250
SLGCVLYEVL TGEPPFTGDS PVSVAYQHVR EDPIPPSARH EGLSADLDAV
260 270 280 290 300
VLKALAKNPE NRYQTAAEMR ADLVRVHNGE PPEAPKVLTD AERTSLLSSA
310 320 330 340 350
AGNLSGPRTD PLPRQDLDDT DRDRSIGSVG RWVAVVAVLA VLTVVVTIAI
360 370 380 390 400
NTFGGITRDV QVPDVRGQSS ADAIATLQNR GFKIRTLQKP DSTIPPDHVI
410 420 430 440 450
GTDPAANTSV SAGDEITVNV STGPEQREIP DVSTLTYAEA VKKLTAAGFG
460 470 480 490 500
RFKQANSPST PELVGKVIGT NPPANQTSAI TNVVIIIVGS GPATKDIPDV
510 520 530 540 550
AGQTVDVAQK NLNVYGFTKF SQASVDSPRP AGEVTGTNPP AGTTVPVDSV
560 570 580 590 600
IELQVSKGNQ FVMPDLSGMF WVDAEPRLRA LGWTGMLDKG ADVDAGGSQH
610 620
NRVVYQNPPA GTGVNRDGII TLRFGQ
Length:626
Mass (Da):66,510
Last modified:March 15, 2005 - v1
Checksum:i6C27EEBE9D5A453B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41247.1.
RefSeqiNP_853684.1. NC_002945.3.
WP_003400356.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO41247; CDO41247; Mb0014c.
GeneIDi1090759.
KEGGimbo:Mb0014c.
PATRICi18001697. VBIMycBov88188_0018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41247.1.
RefSeqiNP_853684.1. NC_002945.3.
WP_003400356.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP0A5S5.
SMRiP0A5S5. Positions 2-299, 355-626.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

BindingDBiP0A5S5.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO41247; CDO41247; Mb0014c.
GeneIDi1090759.
KEGGimbo:Mb0014c.
PATRICi18001697. VBIMycBov88188_0018.

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000037185.
KOiK08884.
OMAiPANIMIN.
OrthoDBiEOG6B35XT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiPKNB_MYCBO
AccessioniPrimary (citable) accession number: P0A5S5
Secondary accession number(s): P71584, X2BDS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: June 24, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.