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Protein

Serine/threonine-protein kinase PknB

Gene

pknB

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Protein kinase that regulates many aspects of mycobacterial physiology. Is a key component of a signal transduction pathway that regulates cell growth, cell shape and cell division via phosphorylation of target proteins.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40ATPPROSITE-ProRule annotation1
Active sitei138Proton acceptorPROSITE-ProRule annotation1
Metal bindingi143MagnesiumBy similarity1
Metal bindingi156MagnesiumBy similarity1
Binding sitei156ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi17 – 25ATPPROSITE-ProRule annotation9
Nucleotide bindingi93 – 95ATPPROSITE-ProRule annotation3
Nucleotide bindingi140 – 143ATPPROSITE-ProRule annotation4

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PknB (EC:2.7.11.1)
Gene namesi
Name:pknB
Ordered Locus Names:Mb0014c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
Proteomesi
  • UP000001419 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 332CytoplasmicSequence analysisAdd BLAST332
Transmembranei333 – 353HelicalSequence analysisAdd BLAST21
Topological domaini354 – 626ExtracellularSequence analysisAdd BLAST273

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712061 – 626Serine/threonine-protein kinase PknBAdd BLAST626

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei166Phosphoserine; by autocatalysisBy similarity1
Modified residuei169Phosphoserine; by autocatalysisBy similarity1
Modified residuei171Phosphothreonine; by autocatalysisBy similarity1
Modified residuei173Phosphothreonine; by autocatalysisBy similarity1
Modified residuei294Phosphothreonine; by autocatalysisBy similarity1
Modified residuei295Phosphoserine; by autocatalysisBy similarity1
Modified residuei309Phosphothreonine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated. Dephosphorylated by PstP (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliP0A5S5.
SMRiP0A5S5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 274Protein kinasePROSITE-ProRule annotationAdd BLAST264
Domaini356 – 422PASTA 1PROSITE-ProRule annotationAdd BLAST67
Domaini423 – 490PASTA 2PROSITE-ProRule annotationAdd BLAST68
Domaini491 – 557PASTA 3PROSITE-ProRule annotationAdd BLAST67
Domaini558 – 626PASTA 4PROSITE-ProRule annotationAdd BLAST69

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 4 PASTA domains.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000037185.
KOiK12132.
OMAiIRMYIND.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P0A5S5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPSHLSDR YELGEILGFG GMSEVHLARD LRLHRDVAVK VLRADLARDP
60 70 80 90 100
SFYLRFRREA QNAAALNHPA IVAVYDTGEA ETPAGPLPYI VMEYVDGVTL
110 120 130 140 150
RDIVHTEGPM TPKRAIEVIA DACQALNFSH QNGIIHRDVK PANIMISATN
160 170 180 190 200
AVKVMDFGIA RAIADSGNSV TQTAAVIGTA QYLSPEQARG DSVDARSDVY
210 220 230 240 250
SLGCVLYEVL TGEPPFTGDS PVSVAYQHVR EDPIPPSARH EGLSADLDAV
260 270 280 290 300
VLKALAKNPE NRYQTAAEMR ADLVRVHNGE PPEAPKVLTD AERTSLLSSA
310 320 330 340 350
AGNLSGPRTD PLPRQDLDDT DRDRSIGSVG RWVAVVAVLA VLTVVVTIAI
360 370 380 390 400
NTFGGITRDV QVPDVRGQSS ADAIATLQNR GFKIRTLQKP DSTIPPDHVI
410 420 430 440 450
GTDPAANTSV SAGDEITVNV STGPEQREIP DVSTLTYAEA VKKLTAAGFG
460 470 480 490 500
RFKQANSPST PELVGKVIGT NPPANQTSAI TNVVIIIVGS GPATKDIPDV
510 520 530 540 550
AGQTVDVAQK NLNVYGFTKF SQASVDSPRP AGEVTGTNPP AGTTVPVDSV
560 570 580 590 600
IELQVSKGNQ FVMPDLSGMF WVDAEPRLRA LGWTGMLDKG ADVDAGGSQH
610 620
NRVVYQNPPA GTGVNRDGII TLRFGQ
Length:626
Mass (Da):66,510
Last modified:March 15, 2005 - v1
Checksum:i6C27EEBE9D5A453B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41247.1.
RefSeqiNP_853684.1. NC_002945.3.
WP_003400356.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO41247; CDO41247; Mb0014c.
GeneIDi1090759.
KEGGimbo:Mb0014c.
PATRICi18001697. VBIMycBov88188_0018.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41247.1.
RefSeqiNP_853684.1. NC_002945.3.
WP_003400356.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP0A5S5.
SMRiP0A5S5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO41247; CDO41247; Mb0014c.
GeneIDi1090759.
KEGGimbo:Mb0014c.
PATRICi18001697. VBIMycBov88188_0018.

Phylogenomic databases

HOGENOMiHOG000037185.
KOiK12132.
OMAiIRMYIND.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR005543. PASTA_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF03793. PASTA. 4 hits.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00740. PASTA. 4 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51178. PASTA. 4 hits.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPKNB_MYCBO
AccessioniPrimary (citable) accession number: P0A5S5
Secondary accession number(s): P71584, X2BDS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.