P0A5R5 (PCP_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyrrolidone-carboxylate peptidase
EC=3.4.19.3

Alternative name(s):
5-oxoprolyl-peptidase
Pyroglutamyl-peptidase I
Short name=PGP-I
Short name=Pyrase

Gene names

Name:	pcp
Ordered Locus Names:	Mb0327

Organism

Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]

Taxonomic identifier

233413 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex › Mycobacterium bovis

Protein attributes

Sequence length	222 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes 5-oxoproline from various penultimate amino acid residues except L-proline By similarity. HAMAP-Rule MF_00417
Catalytic activity	Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro. HAMAP-Rule MF_00417
Subunit structure	Homotetramer By similarity. HAMAP-Rule MF_00417
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00417.
Sequence similarities	Belongs to the peptidase C15 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW pyroglutamyl-peptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 222	222	Pyrrolidone-carboxylate peptidase HAMAP-Rule MF_00417		PRO_0000184723
Sites
	Active site	80	1	By similarity
	Active site	146	1	By similarity
	Active site	170	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5R5 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: C542D9FD2CE2477D	FASTA	222	23,193
	10 20 30 40 50 60 MSKVLVTGFG PYGVTPVNPA QLTAEELDGR TIAGATVISR IVPNTFFESI AAAQQAIAEI 70 80 90 100 110 120 EPALVIMLGE YPGRSMITVE RLAQNVNDCG RYGLADCAGR VLVGEPTDPA GPVAYHATVP 130 140 150 160 170 180 VRAMVLAMRK AGVPADVSDA AGTFVCNHLM YGVLHHLAQK GLPVRAGWIH LPCLPSVAAL 190 200 210 220 DHNLGVPSMS VQTAVAGVTA GIEAAIRQSA DIREPIPSRL QI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of the pcp gene from Mycobacterium bovis BCG." Kim J.K., Choe Y.K. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BCG / Pasteur.
[2]	"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U91845 Genomic DNA. Translation: AAB63524.1. BX248335 Genomic DNA. Translation: CAD93191.1.
RefSeq	NP_853991.1. NC_002945.3.
3D structure databases
ProteinModelPortal	P0A5R5.
SMR	P0A5R5. Positions 4-205.
ModBase	Search...
Protein-protein interaction databases
STRING	233413.Mb0327.
Protein family/group databases
MEROPS	C15.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAD93191; CAD93191; Mb0327.
GeneID	1091190.
KEGG	mbo:Mb0327.
PATRIC	18002376. VBIMycBov88188_0357.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2039.
HOGENOM	HOG000242641.
KO	K01304.
OMA	AYFTQLP.
ProtClustDB	PRK13195.
Family and domain databases
Gene3D	3.40.630.20. 1 hit.
HAMAP	MF_00417. Pyrrolid_peptidase.
InterPro	IPR000816. Peptidase_C15. IPR016125. Peptidase_C15-like. [Graphical view]
PANTHER	PTHR23402. PTHR23402. 1 hit.
Pfam	PF01470. Peptidase_C15. 1 hit. [Graphical view]
PIRSF	PIRSF015592. Prld-crbxl_pptds. 1 hit.
PRINTS	PR00706. PYROGLUPTASE.
SUPFAM	SSF53182. Peptidase_C15-like. 1 hit.
TIGRFAMs	TIGR00504. pyro_pdase. 1 hit.
PROSITE	PS01334. PYRASE_CYS. 1 hit. PS01333. PYRASE_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PCP_MYCBO

Accession

Primary (citable) accession number: P0A5R5
Secondary accession number(s): O07930

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program