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Protein

Cyclopropane mycolic acid synthase 2

Gene

cmaA2

Organism
Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of trans cyclopropanated ketomycolate or methoxymycolate through the conversion of a double bond to a cyclopropane ring at the proximal position of an oxygenated mycolic acid via the transfer of a methylene group from S-adenosyl-L-methionine. In the absence of MmaA2, CmaA2 has a non-specific cis-cyclopropanating activity and is able to catalyze the conversion of a double bond to a cis cyclopropane ring at the distal position of an alpha mycolic acid. Cyclopropanated mycolic acids are key factors participating in cell envelope permeability, host immunomodulation and persistence (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei284 – 2841By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00915.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclopropane mycolic acid synthase 2 (EC:2.1.1.79)
Short name:
CMAS
Alternative name(s):
Cyclopropane-fatty-acyl-phospholipid synthase
Short name:
CFA synthase
Short name:
Cyclopropane fatty acid synthase
Mycolic acid methyltransferase
Short name:
MA-MT
S-adenosylmethionine-dependent methyltransferase
Short name:
AdoMet-MT
Short name:
SAM-MT
Gene namesi
Name:cmaA2
Synonyms:cma2
Ordered Locus Names:Mb0515c
OrganismiMycobacterium bovis (strain ATCC BAA-935 / AF2122/97)
Taxonomic identifieri233413 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeMycobacteriaceaeMycobacteriumMycobacterium tuberculosis complex
ProteomesiUP000001419 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 302302Cyclopropane mycolic acid synthase 2PRO_0000089567Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi233413.Mb0515c.

Structurei

3D structure databases

ProteinModelPortaliP0A5P1.
SMRiP0A5P1. Positions 12-302.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 422S-adenosyl-L-methionine bindingBy similarity
Regioni76 – 849S-adenosyl-L-methionine bindingBy similarity
Regioni102 – 1076S-adenosyl-L-methionine bindingBy similarity
Regioni131 – 1322S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the CFA/CMAS family.Curated

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
KOiK00574.
OMAiWKQADTL.
OrthoDBiEOG6BGNZP.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.

Sequencei

Sequence statusi: Complete.

P0A5P1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSQGDTTSG TQLKPPVEAV RSHYDKSNEF FKLWLDPSMT YSCAYFERPD
60 70 80 90 100
MTLEEAQYAK RKLALDKLNL EPGMTLLDIG CGWGSTMRHA VAEYDVNVIG
110 120 130 140 150
LTLSENQYAH DKAMFDEVDS PRRKEVRIQG WEEFDEPVDR IVSLGAFEHF
160 170 180 190 200
ADGAGDAGFE RYDTFFKKFY NLTPDDGRML LHTITIPDKE EAQELGLTSP
210 220 230 240 250
MSLLRFIKFI LTEIFPGGRL PRISQVDYYS SNAGWKVERY HRIGANYVPT
260 270 280 290 300
LNAWADALQA HKDEAIALKG QETYDIYMHY LRGCSDLFRD KYTDVCQFTL

VK
Length:302
Mass (Da):34,660
Last modified:March 15, 2005 - v1
Checksum:i63AAA95627F95755
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41756.1.
RefSeqiNP_854178.1. NC_002945.3.
WP_003402621.1. NC_002945.3.

Genome annotation databases

EnsemblBacteriaiCDO41756; CDO41756; Mb0515c.
GeneIDi1091574.
KEGGimbo:Mb0515c.
PATRICi18002785. VBIMycBov88188_0561.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX248333 Genomic DNA. Translation: CDO41756.1.
RefSeqiNP_854178.1. NC_002945.3.
WP_003402621.1. NC_002945.3.

3D structure databases

ProteinModelPortaliP0A5P1.
SMRiP0A5P1. Positions 12-302.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi233413.Mb0515c.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCDO41756; CDO41756; Mb0515c.
GeneIDi1091574.
KEGGimbo:Mb0515c.
PATRICi18002785. VBIMycBov88188_0561.

Phylogenomic databases

eggNOGiCOG2230.
HOGENOMiHOG000245191.
KOiK00574.
OMAiWKQADTL.
OrthoDBiEOG6BGNZP.

Enzyme and pathway databases

UniPathwayiUPA00915.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR003333. Mycolic_cyclopropane_synthase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF02353. CMAS. 1 hit.
[Graphical view]
PIRSFiPIRSF003085. CMAS. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-935 / AF2122/97.

Entry informationi

Entry nameiCMAS2_MYCBO
AccessioniPrimary (citable) accession number: P0A5P1
Secondary accession number(s): Q11196, X2BFA2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 27, 2015
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.