Reviewed, UniProtKB/Swiss-Prot P0A5N5 (AHPD_MYCBO)

Names and origin

Protein names

Recommended name:
    Alkyl hydroperoxide reductase ahpD
    EC=1.11.1.15
Alternative name(s):
    Alkylhydroperoxidase ahpD

Gene names

Name:	ahpD
Ordered Locus Names:	Mb2455

Organism

Mycobacterium bovis [Complete proteome] [HAMAP]

Taxonomic identifier

1765 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Antioxidant protein with alkyl hydroperoxidase activity. Required for the reduction of the ahpC active site cysteine residues and for the regeneration of the ahpC enzyme activity By similarity. HAMAP MF_01676
Catalytic activity	2 R'-SH + ROOH = R'-S-S-R' + H2O + ROH. HAMAP MF_01676
Subunit structure	Homotrimer By similarity. HAMAP MF_01676
Sequence similarities	Belongs to the ahpD family.

Ontologies

Keywords
Domain	Redox-active center
Molecular function	Antioxidant Oxidoreductase Peroxidase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkyl hydroperoxide reductase activity Inferred from electronic annotation. Source: HAMAP peroxiredoxin activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Alkyl hydroperoxide reductase ahpD HAMAP MF_01676		PRO_0000064505
Sites
	Active site	130	1	Proton donor By similarity
	Active site	133	1	Cysteine sulfenic acid (-SOH) intermediate By similarity
Amino acid modifications
	Disulfide bond	130 ↔ 133		Alternate By similarity
	Disulfide bond	133		Interchain (with ahpC); in linked form By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5N5-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: B7DD28AA9BD9B24C	FASTA	177	18,781
	10 20 30 40 50 60 MSIEKLKAAL PEYAKDIKLN LSSITRSSVL DQEQLWGTLL ASAAATRNPQ VLADIGAEAT 70 80 90 100 110 120 DHLSAAARHA ALGAAAIMGM NNVFYRGRGF LEGRYDDLRP GLRMNIIANP GIPKANFELW 130 140 150 160 170 SFAVSAINGC SHCLVAHEHT LRTVGVDREA IFEALKAAAI VSGVAQALAT IEALSPS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ahpC, a gene involved in isoniazid resistance of the Mycobacterium tuberculosis complex." Wilson T.M., Collins D.M. Mol. Microbiol. 19:1025-1034(1996) [PubMed: 8830260] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35723 / TMC 405 and ATCC 35729 / TMC 606.
[2]	"The complete genome sequence of Mycobacterium bovis." Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M., Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B., Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J. , Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G. Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003) [PubMed: 12788972] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-935 / AF2122/97.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24083 Genomic DNA. Translation: AAB60204.1. U24084 Genomic DNA. Translation: AAB38113.1. BX248342 Genomic DNA. Translation: CAD97316.1.
PIR	S71014.
RefSeq	NP_856102.1.
3D structure databases
SMR	P0A5N5. Positions 2-175.
ModBase	Search...
Protein family/group databases
PeroxiBase	4577. MboAhpD.
Genome annotation databases
GeneID	1094261.
KEGG	mbo:Mb2455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG414634.
OMA	RAGLRMN.
Enzyme and pathway databases
BioCyc	MBOV233413:MB2455-MONOMER.
Family and domain databases
HAMAP	MF_01676. AhpD. [Tree]
InterPro	IPR004674. AhpD. IPR004675. AhpD_core. IPR003779. CMD. [Graphical view]
Pfam	PF02627. CMD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00777. ahpD. 1 hit. TIGR00778. ahpD_dom. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AHPD_MYCBO

Accession

Primary (citable) accession number: P0A5N5
Secondary accession number(s): Q57353

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	February 9, 2010
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)