Reviewed, UniProtKB/Swiss-Prot P0A5L8 (NAT_MYCTU)

Names and origin

Protein names

Recommended name:
    Arylamine N-acetyltransferase
    EC=2.3.1.5

Gene names

Name:	nat
Ordered Locus Names:	Rv3566c, MT3671
ORF Names:	MTCY06G11.13c

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Could have a role in acetylating, and hence inactivating, the antitubercular drug isoniazid.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arylamine N-acetyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Arylamine N-acetyltransferase		PRO_0000107919
Sites
	Active site	70	1	Acyl-thioester intermediate By similarity
	Active site	110	1	By similarity
	Active site	127	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5L8-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 9C8D98E3256D088A	FASTA	283	31,029
	10 20 30 40 50 60 MALDLTAYFD RINYRGATDP TLDVLQDLVT VHSRTIPFEN LDPLLGVPVD DLSPQALADK 70 80 90 100 110 120 LVLRRRGGYC FEHNGLMGYV LAELGYRVRR FAARVVWKLA PDAPLPPQTH TLLGVTFPGS 130 140 150 160 170 180 GGCYLVDVGF GGQTPTSPLR LETGAVQPTT HEPYRLEDRV DGFVLQAMVR DTWQTLYEFT 190 200 210 220 230 240 TQTRPQIDLK VASWYASTHP ASKFVTGLTA AVITDDARWN LSGRDLAVHR AGGTEKIRLA 250 260 270 280 DAAAVVDTLS ERFGINVADI GERGALETRI DELLARQPGA DAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv." Camus J.-C., Pryor M.J., Medigue C., Cole S.T. Microbiology 148:2967-2973(2002) [PubMed: 12368430] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Cloning and characterization of arylamine N-acetyltransferase genes from Mycobacterium smegmatis and Mycobacterium tuberculosis: increased expression results in isoniazid resistance." Payton M.A., Auty R., Delgoda R.T., Everitt M., Sim E. J. Bacteriol. 181:1343-1347(1999) [PubMed: 9973365] [Abstract] Cited for: CHARACTERIZATION.
[5]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed: 19099550] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842583 Genomic DNA. Translation: CAE55614.1. AE000516 Genomic DNA. Translation: AAK48028.1.
RefSeq	NP_338214.1. YP_177989.1.
3D structure databases
SMR	P0A5L8. Positions 4-273.
ModBase	Search...
Genome annotation databases
GeneID	888005. 926546.
GenomeReviews	Gene locus MT3671 in contig AE000516_GR.
KEGG	mtc:MT3671. mtu:Rv3566c.
TIGR	MT3671.
Organism-specific databases
TubercuList	Rv3566c.
Phylogenomic databases
HOGENOM	HBG544563.
OMA	WYTSTHP.
Enzyme and pathway databases
BRENDA	2.3.1.5. 809.
Family and domain databases
InterPro	IPR001447. N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. Acetyltransf2. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAT_MYCTU

Accession

Primary (citable) accession number: P0A5L8
Secondary accession number(s): P96848

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	February 9, 2010
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)