P0A5L8 (NAT_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arylamine N-acetyltransferase
EC=2.3.1.5

Gene names

Name:	nat
Ordered Locus Names:	Rv3566c, MT3671
ORF Names:	MTCY06G11.13c

Organism

Mycobacterium tuberculosis [Reference proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	283 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Could have a role in acetylating, and hence inactivating, the antitubercular drug isoniazid.
Catalytic activity	Acetyl-CoA + an arylamine = CoA + an N-acetylarylamine.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Was identified as a high-confidence drug target.
Sequence similarities	Belongs to the arylamine N-acetyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 15525680. Source: MTBBASE
Molecular_function	arylamine N-acetyltransferase activity Inferred from direct assay PubMed 18795795Ref.4. Source: MTBBASE
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 283	283	Arylamine N-acetyltransferase		PRO_0000107919
Sites
	Active site	70	1	Acyl-thioester intermediate By similarity
	Active site	110	1	By similarity
	Active site	127	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P0A5L8 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 9C8D98E3256D088A	FASTA	283	31,029
	10 20 30 40 50 60 MALDLTAYFD RINYRGATDP TLDVLQDLVT VHSRTIPFEN LDPLLGVPVD DLSPQALADK 70 80 90 100 110 120 LVLRRRGGYC FEHNGLMGYV LAELGYRVRR FAARVVWKLA PDAPLPPQTH TLLGVTFPGS 130 140 150 160 170 180 GGCYLVDVGF GGQTPTSPLR LETGAVQPTT HEPYRLEDRV DGFVLQAMVR DTWQTLYEFT 190 200 210 220 230 240 TQTRPQIDLK VASWYASTHP ASKFVTGLTA AVITDDARWN LSGRDLAVHR AGGTEKIRLA 250 260 270 280 DAAAVVDTLS ERFGINVADI GERGALETRI DELLARQPGA DAP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv." Camus J.-C., Pryor M.J., Medigue C., Cole S.T. Microbiology 148:2967-2973(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.
[3]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[4]	"Cloning and characterization of arylamine N-acetyltransferase genes from Mycobacterium smegmatis and Mycobacterium tuberculosis: increased expression results in isoniazid resistance." Payton M.A., Auty R., Delgoda R.T., Everitt M., Sim E. J. Bacteriol. 181:1343-1347(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"targetTB: a target identification pipeline for Mycobacterium tuberculosis through an interactome, reactome and genome-scale structural analysis." Raman K., Yeturu K., Chandra N. BMC Syst. Biol. 2:109-109(2008) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A DRUG TARGET [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX842583 Genomic DNA. Translation: CAE55614.1. AE000516 Genomic DNA. Translation: AAK48028.1. AL123456 Genomic DNA. Translation: CCP46388.1.
RefSeq	NP_338214.1. NC_002755.2. YP_006517055.1. NC_018143.1. YP_177989.1. NC_000962.3.
3D structure databases
ProteinModelPortal	P0A5L8.
SMR	P0A5L8. Positions 4-273.
ModBase	Search...
Protein-protein interaction databases
STRING	83332.Rv3566c.
Proteomic databases
PRIDE	P0A5L8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAK48028; AAK48028; MT3671.
GeneID	13317174. 888005. 926546.
KEGG	mtc:MT3671. mtu:Rv3566c. mtv:RVBD_3566c.
PATRIC	18129827. VBIMycTub22151_4008.
Organism-specific databases
TubercuList	Rv3566c.
Phylogenomic databases
eggNOG	COG2162.
HOGENOM	HOG000205436.
KO	K00622.
OMA	LANPPQM.
ProtClustDB	CLSK800002.
Family and domain databases
InterPro	IPR001447. Arylamine_N-AcTrfase. [Graphical view]
PANTHER	PTHR11786. PTHR11786. 1 hit.
Pfam	PF00797. Acetyltransf_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NAT_MYCTU

Accession

Primary (citable) accession number: P0A5L8
Secondary accession number(s): L0TCY1, P96848

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	May 1, 2013
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program