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P0A5L7 (NADE_MYCBO) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glutamine-dependent NAD(+) synthetase
EC=6.3.5.1
Alternative name(s):
NAD(+) synthase [glutamine-hydrolyzing]
Gene names
Name:nadE
Ordered Locus Names:Mb2464c
OrganismMycobacterium bovis (strain ATCC BAA-935 / AF2122/97) [Complete proteome] [HAMAP]
Taxonomic identifier233413 [NCBI]
Taxonomic lineageBacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex ›

Protein attributes

Sequence length679 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Can use both glutamine or ammonia as a nitrogen source By similarity. HAMAP-Rule MF_00193

Catalytic activity

ATP + deamido-NAD+ + L-glutamine + H2O = AMP + diphosphate + NAD+ + L-glutamate. HAMAP-Rule MF_00193

Pathway

Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (L-Gln route): step 1/1. HAMAP-Rule MF_00193

Sequence similarities

In the C-terminal section; belongs to the NAD synthetase family.

Contains 1 CN hydrolase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 679679Glutamine-dependent NAD(+) synthetase HAMAP-Rule MF_00193
PRO_0000152242

Regions

Domain12 – 298287CN hydrolase
Nucleotide binding366 – 3738ATP By similarity
Region337 – 679343Ligase HAMAP-Rule MF_00193

Sites

Active site3681 By similarity

Sequences

Sequence LengthMass (Da)Tools
P0A5L7 [UniParc].

Last modified March 15, 2005. Version 1.
Checksum: 14AC29CE434A8B0D

FASTA67974,683
        10         20         30         40         50         60 
MNFYSAYQHG FVRVAACTHH TTIGDPAANA ASVLDMARAC HDDGAALAVF PELTLSGYSI 

        70         80         90        100        110        120 
EDVLLQDSLL DAVEDALLDL VTESADLLPV LVVGAPLRHR HRIYNTAVVI HRGAVLGVVP 

       130        140        150        160        170        180 
KSYLPTYREF YERRQMAPGD GERGTIRIGG ADVAFGTDLL FAASDLPGFV LHVEICEDMF 

       190        200        210        220        230        240 
VPMPPSAEAA LAGATVLANL SGSPITIGRA EDRRLLARSA SARCLAAYVY AAAGEGESTT 

       250        260        270        280        290        300 
DLAWDGQTMI WENGALLAES ERFPKGVRRS VADVDTELLR SERLRMGTFD DNRRHHRELT 

       310        320        330        340        350        360 
ESFRRIDFAL DPPAGDIGLL REVERFPFVP ADPQRLQQDC YEAYNIQVSG LEQRLRALDY 

       370        380        390        400        410        420 
PKVVIGVSGG LDSTHALIVA THAMDREGRP RSDILAFALP GFATGEHTKN NAIKLARALG 

       430        440        450        460        470        480 
VTFSEIDIGD TARLMLHTIG HPYSVGEKVY DVTFENVQAG LRTDYLFRIA NQRGGIVLGT 

       490        500        510        520        530        540 
GDLSELALGW STYGVGDQMS HYNVNAGVPK TLIQHLIRWV ISAGEFGEKV GEVLQSVLDT 

       550        560        570        580        590        600 
EITPELIPTG EEELQSSEAK VGPFALQDFS LFQVLRYGFR PSKIAFLAWH AWNDAERGNW 

       610        620        630        640        650        660 
PPGFPKSERP SYSLAEIRHW LQIFVQRFYS FSQFKRSALP NGPKVSHGGA LSPRGDWRAP 

       670 
SDMSARIWLD QIDREVPKG

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BX248342 Genomic DNA. Translation: CAD97325.1.
RefSeqNP_856111.1. NC_002945.3.

3D structure databases

ProteinModelPortalP0A5L7.
SMRP0A5L7. Positions 1-679.
ModBaseSearch...

Protein-protein interaction databases

STRING233413.Mb2464c.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAD97325; CAD97325; Mb2464c.
GeneID1094244.
KEGGmbo:Mb2464c.
PATRIC18007139. VBIMycBov88188_2712.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0388.
HOGENOMHOG000051304.
KOK01950.
OMAFLALHAW.
ProtClustDBPRK02628.

Enzyme and pathway databases

UniPathwayUPA00253; UER00334.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.60.110.10. 1 hit.
HAMAPMF_00193. NadE. Fused.
InterProIPR003010. C-N_Hydrolase.
IPR014445. Gln-dep_NAD_synthase.
IPR022310. NAD/GMP_synthase.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00795. CN_hydrolase. 1 hit.
PF02540. NAD_synthase. 1 hit.
[Graphical view]
PIRSFPIRSF006630. NADS_GAT. 1 hit.
SUPFAMSSF56317. Ntlse/CNhydtse. 1 hit.
PROSITEPS50263. CN_HYDROLASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNADE_MYCBO
AccessionPrimary (citable) accession number: P0A5L7
Secondary accession number(s): P71911
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: May 1, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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